QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kuhlemeier, C Articles by Chua, N H Search for Related Content PubMed PubMed Citation Articles by Kuhlemeier, C Articles by Chua, N H Social Bookmarking                   What's this?

Research Papers

Sequences in the pea rbcS-3A gene have homology to constitutive mammalian enhancers but function as negative regulatory elements.

Laboratory of Plant Molecular Biology, Rockefeller University, New York, New York 10021-6399.

Abstract

The pea rbcS-3A gene, which codes for a key component of the photosynthetic machinery, requires light for its expression. Analysis of chimeric constructs in transgenic tobacco plants has shown that a 280-bp fragment from the 5' noncoding region can act as a light-inducible transcriptional enhancer. Further characterization of this enhancer identifies a 58-bp sequence containing two regulatory elements that can decrease transcription in the dark. One has a high degree of homology to the SV40 core enhancer, the other to the adenovirus 5 E1A enhancer and the constitutive part of the human interferon-beta gene enhancer. Deletion of the 58-bp sequence uncovers additional light-responsive elements (LREs) located further upstream and downstream.

CiteULike

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				P. A. Manavella, C. A. Dezar, F. D. Ariel, M. F. Drincovich, and R. L. Chan The sunflower HD-Zip transcription factor HAHB4 is up-regulated in darkness, reducing the transcription of photosynthesis-related genes J. Exp. Bot., July 4, 2008; (2008) ern170v1. [Abstract] [Full Text] [PDF]

				K.-i. Kucho, S. Yoshioka, F. Taniguchi, K. Ohyama, and H. Fukuzawa Cis-acting Elements and DNA-Binding Proteins Involved in CO2-Responsive Transcriptional Activation of Cah1 Encoding a Periplasmic Carbonic Anhydrase in Chlamydomonas reinhardtii Plant Physiology, October 1, 2003; 133(2): 783 - 793. [Abstract] [Full Text] [PDF]

				S.-W. Jiang and N. L. Eberhardt The Human Chorionic Somatomammotropin Enhancers Form a Composite Silencer in Pituitary Cells in Vitro Mol. Endocrinol., August 1, 1997; 11(9): 1233 - 1244. [Abstract] [Full Text]

				E Lam and N. Chua GT-1 binding site confers light responsive expression in transgenic tobacco Science, April 27, 1990; 248(4954): 471 - 474. [Abstract] [PDF]

				S K Logan and P C Wensink Ovarian follicle cell enhancers from the Drosophila yolk protein genes: different segments of one enhancer have different cell-type specificities that interact to give normal expression. Genes & Dev., April 1, 1990; 4(4): 613 - 623. [Abstract] [PDF]

				P. N. BENFEY and N.-H. CHUA Regulated Genes in Transgenic Plants Science, April 14, 1989; 244(4901): 174 - 181. [Abstract] [PDF]

				F. Nagy, S. A. Kay, and N.-H. Chua A circadian clock regulates transcription of the wheat Cab-1 gene Genes & Dev., April 1, 1988; 2(4): 376 - 382. [Abstract] [PDF]