![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Research Papers
Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA.
Abstract
The single amino acid "P" (potentiator) mutation in the holoenzyme component GAL11 creates an interaction between that protein and the dimerization region of GAL4. That interaction triggers strong gene activation when the GAL4 fragment is tethered to DNA. Here we show that, among a series of variants of the GAL4 dimerization region and different GAL11P alleles, the strength of the interaction as quantitated in vitro correlates with the degree of activation in vivo; swapping the protein fragments bearing the GAL4 dimerization region and the GAL11P mutation such that the latter is tethered to DNA and the former is attached to the holoenzyme does not diminish gene activation; gene activation in this system is squelched by overproduction of either a fragment bearing the GAL4 dimerization region or a fragment of GAL11 bearing a P mutation; and neither GAL11 nor GAL11P is a target of an acidic activating region. These results argue that the GAL4-GAL11P interaction triggers gene activation simply by recruiting the holoenzyme to DNA. Consistent with this view, we also show that fusion of LexA to another holoenzyme component, SRB2, creates an activator, and that an SRB2 mutant predicted on genetic grounds to interact especially efficiently with a holoenzyme containing a specific mutant form of polymerase also activates more efficiently when tethered to DNA.
CiteULike 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  W. M. Reeves and S. Hahn Targets of the Gal4 Transcription Activator in Functional Transcription Complexes Mol. Cell. Biol., October 15, 2005; 25(20): 9092 - 9102. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Takagi, J. Z. Chadick, J. A. Davis, and F. J. Asturias Preponderance of Free Mediator in the Yeast Saccharomyces cerevisiae J. Biol. Chem., September 2, 2005; 280(35): 31200 - 31207. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Lu, S. P. Rowe, B. B. Brennan, S. E. Davis, R. E. Metzler, J. J. Nau, C. Y. Majmudar, A. K. Mapp, and A. Z. Ansari Unraveling the Mechanism of a Potent Transcriptional Activator J. Biol. Chem., August 19, 2005; 280(33): 29689 - 29698. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Zhao and A. Dean An insulator blocks spreading of histone acetylation and interferes with RNA polymerase II transfer between an enhancer and gene Nucleic Acids Res., September 15, 2004; 32(16): 4903 - 4919. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Park, J. M. Kim, L. K. Kim, S. N. Kim, J. Kim-Ha, J. H. Kim, and Y.-J. Kim Signal-Induced Transcriptional Activation by Dif Requires the dTRAP80 Mediator Module Mol. Cell. Biol., February 15, 2003; 23(4): 1358 - 1367. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Balciunas, M. Hallberg, S. Bjorklund, and H. Ronne Functional Interactions within Yeast Mediator and Evidence of Differential Subunit Modifications J. Biol. Chem., January 31, 2003; 278(6): 3831 - 3839. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. A. d. Maur, C. Zahnd, F. Fischer, S. Spinelli, A. Honegger, C. Cambillau, D. Escher, A. Pluckthun, and A. Barberis Direct in Vivo Screening of Intrabody Libraries Constructed on a Highly Stable Single-chain Framework J. Biol. Chem., November 15, 2002; 277(47): 45075 - 45085. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D.-Y. Zhang, D. J. Carson, and J. Ma The role of TFIIB-RNA polymerase II interaction in start site selection in yeast cells Nucleic Acids Res., July 15, 2002; 30(14): 3078 - 3085. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Lu, A. Z. Ansari, X. Lu, A. Ogirala, and M. Ptashne A target essential for the activity of a nonacidic yeast transcriptional activator PNAS, June 25, 2002; 99(13): 8591 - 8596. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Traven, L. Staresincic, M. Arneric, and M. Sopta The yeast protein Xtc1 functions as a direct transcriptional repressor Nucleic Acids Res., June 1, 2002; 30(11): 2358 - 2364. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. G. West, M. Gaszner, and G. Felsenfeld Insulators: many functions, many mechanisms Genes & Dev., February 1, 2002; 16(3): 271 - 288. [Full Text] [PDF]
|
|
|
|
|
|
M. Bodmer-Glavas, K. Edler, and A. Barberis RNA polymerase II and III transcription factors can stimulate DNA replication by modifying origin chromatin structures Nucleic Acids Res., November 15, 2001; 29(22): 4570 - 4580. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Hermann, K. D. Berndt, and A. P. Wright How Transcriptional Activators Bind Target Proteins J. Biol. Chem., October 19, 2001; 276(43): 40127 - 40132. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. L. Jenkins and C. A. Spencer RNA Polymerase II Holoenzyme Modifications Accompany Transcription Reprogramming in Herpes Simplex Virus Type 1-Infected Cells J. Virol., October 15, 2001; 75(20): 9872 - 9884. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. T. Bhoite, Y. Yu, and D. J. Stillman The Swi5 activator recruits the Mediator complex to the HO promoter without RNA polymerase II Genes & Dev., September 15, 2001; 15(18): 2457 - 2469. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. A. Stafford and R. H. Morse GCN5 Dependence of Chromatin Remodeling and Transcriptional Activation by the GAL4 and VP16 Activation Domains in Budding Yeast Mol. Cell. Biol., July 15, 2001; 21(14): 4568 - 4578. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Hidalgo, A. Z. Ansari, P. Schmidt, B. Hare, N. Simkovich, S. Farrell, E. J. Shin, M. Ptashne, and G. Wagner Recruitment of the transcriptional machinery through GAL11P: structure and interactions of the GAL4 dimerization domain Genes & Dev., April 15, 2001; 15(8): 1007 - 1020. [Abstract] [Full Text]
|
|
|
|
|
|
S. M. Kraemer, R. T. Ranallo, R. C. Ogg, and L. A. Stargell TFIIA Interacts with TFIID via Association with TATA-Binding Protein and TAF40 Mol. Cell. Biol., March 1, 2001; 21(5): 1737 - 1746. [Abstract] [Full Text]
|
|
|
|
|
|
A. J. Shaywitz, S. L. Dove, J. M. Kornhauser, A. Hochschild, and M. E. Greenberg Magnitude of the CREB-Dependent Transcriptional Response Is Determined by the Strength of the Interaction between the Kinase-Inducible Domain of CREB and the KIX Domain of CREB-Binding Protein Mol. Cell. Biol., December 15, 2000; 20(24): 9409 - 9422. [Abstract] [Full Text]
|
|
|
|
|
|
J. M. Park, H.-S. Kim, S. J. Han, M.-S. Hwang, Y. C. Lee, and Y.-J. Kim In Vivo Requirement of Activator-Specific Binding Targets of Mediator Mol. Cell. Biol., December 1, 2000; 20(23): 8709 - 8719. [Abstract] [Full Text]
|
|
|
|
|
|
S. L. Dove, F. W. Huang, and A. Hochschild Mechanism for a transcriptional activator that works at the isomerization step PNAS, November 21, 2000; 97(24): 13215 - 13220. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. S. Kobor, L. D. Simon, J. Omichinski, G. Zhong, J. Archambault, and J. Greenblatt A Motif Shared by TFIIF and TFIIB Mediates Their Interaction with the RNA Polymerase II Carboxy-Terminal Domain Phosphatase Fcp1p in Saccharomyces cerevisiae Mol. Cell. Biol., October 15, 2000; 20(20): 7438 - 7449. [Abstract] [Full Text]
|
|
|
|
|
|
J.-a Kim, J. Lu, and P. G. Quinn Distinct cAMP response element-binding protein (CREB) domains stimulate different steps in a concerted mechanism of transcription activation PNAS, October 10, 2000; 97(21): 11292 - 11296. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. P. Ryan, G. A. Stafford, L. Yu, and R. H. Morse Artificially Recruited TATA-Binding Protein Fails To Remodel Chromatin and Does Not Activate Three Promoters That Require Chromatin Remodeling Mol. Cell. Biol., August 15, 2000; 20(16): 5847 - 5857. [Abstract] [Full Text]
|
|
|
|
|
|
D. R. Dorris and K. Struhl Artificial Recruitment of TFIID, but Not RNA Polymerase II Holoenzyme, Activates Transcription in Mammalian Cells Mol. Cell. Biol., June 15, 2000; 20(12): 4350 - 4358. [Abstract] [Full Text]
|
|
|
|
|
|
J. K. Joung, E. I. Ramm, and C. O. Pabo A bacterial two-hybrid selection system for studying protein-DNA and protein-protein interactions PNAS, June 13, 2000; (2000) 110149297. [Abstract] [Full Text]
|
|
|
|
|
|
L. A. Stargell, Z. Moqtaderi, D. R. Dorris, R. C. Ogg, and K. Struhl TFIIA Has Activator-dependent and Core Promoter Functions in Vivo J. Biol. Chem., April 21, 2000; 275(17): 12374 - 12380. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Papamichos-Chronakis, R. S. Conlan, N. Gounalaki, T. Copf, and D. Tzamarias Hrs1/Med3 Is a Cyc8-Tup1 Corepressor Target in the RNA Polymerase II Holoenzyme J. Biol. Chem., March 17, 2000; 275(12): 8397 - 8403. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. W. Phillips, R. Sharma, P. A. Leco, and D. R. Edwards A Sequence-selective Single-strand DNA-binding Protein Regulates Basal Transcription of the Murine Tissue Inhibitor of Metalloproteinases-1 (Timp-1) Gene J. Biol. Chem., August 6, 1999; 274(32): 22197 - 22207. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Kimura, Y. Tao, R. G. Roeder, and P. R. Cook Quantitation of RNA Polymerase II and Its Transcription Factors in an HeLa Cell: Little Soluble Holoenzyme but Significant Amounts of Polymerases Attached to the Nuclear Substructure Mol. Cell. Biol., August 1, 1999; 19(8): 5383 - 5392. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Sune and M. A. Garcia-Blanco Transcriptional Cofactor CA150 Regulates RNA Polymerase II Elongation in a TATA-Box-Dependent Manner Mol. Cell. Biol., July 1, 1999; 19(7): 4719 - 4728. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Gaudreau, M. Keaveney, J. Nevado, Z. Zaman, G. O. Bryant, K. Struhl, and M. Ptashne Transcriptional activation by artificial recruitment in yeast is influenced by promoter architecture and downstream sequences PNAS, March 16, 1999; 96(6): 2668 - 2673. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Nevado, L. Gaudreau, M. Adam, and M. Ptashne Transcriptional activation by artificial recruitment in mammalian cells PNAS, March 16, 1999; 96(6): 2674 - 2677. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Ossipow, P. Fonjallaz, and U. Schibler An RNA Polymerase II Complex Containing All Essential Initiation Factors Binds to the Activation Domain of PAR Leucine Zipper Transcription Factor Thyroid Embryonic Factor Mol. Cell. Biol., February 1, 1999; 19(2): 1242 - 1250. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Kotani, T. Miyake, Y. Tsukihashi, A. G. Hinnebusch, Y. Nakatani, M. Kawaichi, and T. Kokubo Identification of Highly Conserved Amino-terminal Segments of dTAFII230 and yTAFII145 That Are Functionally Interchangeable for Inhibiting TBP-DNA Interactions in Vitro and in Promoting Yeast Cell Growth in Vivo J. Biol. Chem., November 27, 1998; 273(48): 32254 - 32264. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Emili, R. Kobayashi, and C. J. Ingles A novel yeast protein influencing the response of RNA polymerase II to transcriptional activators PNAS, September 15, 1998; 95(19): 11122 - 11127. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. B. McNeil, H. Agah, and D. Bentley Activated transcription independent of the RNA polymerase II holoenzyme in budding yeast Genes & Dev., August 15, 1998; 12(16): 2510 - 2521. [Abstract] [Full Text]
|
|
|
|
|
|
B. Majello, G. Napolitano, P. De Luca, and L. Lania Recruitment of Human TBP Selectively Activates RNA Polymerase II TATA-dependent Promoters J. Biol. Chem., June 26, 1998; 273(26): 16509 - 16516. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. He and S. J. Weintraub Stepwise Recruitment of Components of the Preinitiation Complex by Upstream Activators In Vivo Mol. Cell. Biol., May 1, 1998; 18(5): 2876 - 2883. [Abstract] [Full Text]
|
|
|
|
|
|
M. P. Ryan, R. Jones, and R. H. Morse SWI-SNF Complex Participation in Transcriptional Activation at a Step Subsequent to Activator Binding Mol. Cell. Biol., April 1, 1998; 18(4): 1774 - 1782. [Abstract] [Full Text]
|
|
|
|
|
|
S. Kuchin and M. Carlson Functional Relationships of Srb10-Srb11 Kinase, Carboxy-Terminal Domain Kinase CTDK-I, and Transcriptional Corepressor Ssn6-Tup1 Mol. Cell. Biol., March 1, 1998; 18(3): 1163 - 1171. [Abstract] [Full Text]
|
|
|
|
|
|
C. M. Drysdale, B. M. Jackson, R. McVeigh, E. R. Klebanow, Y. Bai, T. Kokubo, M. Swanson, Y. Nakatani, P. A. Weil, and A. G. Hinnebusch The Gcn4p Activation Domain Interacts Specifically In Vitro with RNA Polymerase II Holoenzyme, TFIID, and the Adap-Gcn5p Coactivator Complex Mol. Cell. Biol., March 1, 1998; 18(3): 1711 - 1724. [Abstract] [Full Text]
|
|
|
|
|
|
S. L. Dove and A. Hochschild Conversion of the omega subunit of Escherichia coli RNA polymerase into a transcriptional activator or an activation target Genes & Dev., March 1, 1998; 12(5): 745 - 754. [Abstract] [Full Text]
|
|
|
|
|
|
H. Sakurai and T. Fukasawa Yeast Gal11 and Transcription Factor IIE Function through a Common Pathway in Transcriptional Regulation J. Biol. Chem., December 19, 1997; 272(51): 32663 - 32669. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Pollock and M. Gilman Transcriptional activation: Is it rocket science? PNAS, December 9, 1997; 94(25): 13388 - 13389. [Full Text] [PDF]
|
|
|
|
|
|
M. Ouhammouch, M. H. Sayre, J. T. Kadonaga, and E. P. Geiduschek Activation of RNA polymerase II by topologically linked DNA-tracking proteins PNAS, June 24, 1997; 94(13): 6718 - 6723. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Scully, S. F. Anderson, D. M. Chao, W. Wei, L. Ye, R. A. Young, D. M. Livingston, and J. D. Parvin BRCA1 is a component of the RNA polymerase II holoenzyme PNAS, May 27, 1997; 94(11): 5605 - 5610. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-Y. Liu, Y.-C. Chiang, J. Pan, J. Chen, C. Salvadore, D. C. Audino, V. Badarinarayana, V. Palaniswamy, B. Anderson, and C. L. Denis Characterization of CAF4 and CAF16 Reveals a Functional Connection between the CCR4-NOT Complex and a Subset of SRB Proteins of the RNA Polymerase II Holoenzyme J. Biol. Chem., March 2, 2001; 276(10): 7541 - 7548. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B.-S. Chen, Z.-W. Sun, and M. Hampsey A Gal4-sigma 54 Hybrid Protein That Functions as a Potent Activator of RNA Polymerase II Transcription in Yeast J. Biol. Chem., June 22, 2001; 276(26): 23881 - 23887. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Lu, A. Z. Ansari, and M. Ptashne An artificial transcriptional activating region with unusual properties PNAS, February 29, 2000; 97(5): 1988 - 1992. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. K. Joung, E. I. Ramm, and C. O. Pabo A bacterial two-hybrid selection system for studying protein-DNA and protein-protein interactions PNAS, June 20, 2000; 97(13): 7382 - 7387. [Abstract] [Full Text] [PDF]
|
|
