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Research Papers
Department of Biological Chemistry, University of California, Los Angeles, School of Medicine, 90095-1737, USA.
Abstract
The prevailing view of eukaryotic gene activation poses that activators stimulate transcription by recruiting limiting components of the general transcription machinery to a core promoter. In one such model case, activation by the Epstein-Barr virus ZEBRA protein correlated closely with recruitment of the general transcription factors TFIIA and TFIID (the DA complex) as measured by DNase I footprinting and gel mobility shift assays. We now report that simple recruitment is not sufficient for full-level activation. An additional concentration-independent, rate-limiting step is activator-mediated isomerization of the DA complex characterized by an extended TFIID footprint. The isomerized complex supports both binding of TFIIB in gel mobility shift assays and activated transcription in heat-treated nuclear extracts, even after removal of ZEBRA. Surprisingly, the regulatory phenomenon of synergy was manifested only when the concentration of TFIID was limiting. When the DA complex was saturating, transcription was not synergistic, as indicated by the ability of a single activator to induce isomerization effectively and turn on a gene. On the basis of these observations, we propose a new biochemical model for eukaryotic gene activation and synergy.
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|
|
|
|
|
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|
|
|
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|
|
|
|
|
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|
|
|
|
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|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
A. Hoffmann, T. Oelgeschlager, and R. G. Roeder Considerations of transcriptional control mechanisms: Do TFIID-core promoter complexes recapitulate nucleosome-like functions? PNAS, August 19, 1997; 94(17): 8928 - 8935. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Gonzalez-Couto, N. Klages, and M. Strubin Synergistic and promoter-selective activation of transcription by recruitment of transcription factors TFIID and TFIIB PNAS, July 22, 1997; 94(15): 8036 - 8041. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G Mengus, M May, L Carre, P Chambon, and I Davidson Human TAF(II)135 potentiates transcriptional activation by the AF-2s of the retinoic acid, vitamin D3, and thyroid hormone receptors in mammalian cells. Genes & Dev., June 1, 1997; 11(11): 1381 - 1395. [Abstract] [PDF]
|
|
|
|
|
|
J. D. Laney and M. D. Biggin Zeste-mediated activation by an enhancer is independent of cooperative DNA binding in vivo PNAS, April 15, 1997; 94(8): 3602 - 3604. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Kim, J. G. Na, M. Hampsey, and D. Reinberg The Dr1/DRAP1 heterodimer is a global repressor of transcription in vivo PNAS, February 4, 1997; 94(3): 820 - 825. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G Orphanides, T Lagrange, and D Reinberg The general transcription factors of RNA polymerase II. Genes & Dev., November 1, 1996; 10(21): 2657 - 2683. [PDF]
|
|
|
|
|
|
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|
|
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