.GIF?ad=16177&adview=true)
![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Research Papers
Institute of Biotechnology, The University of Texas Health Science Center at San Antonio 78245, USA.
Abstract
To define a mechanism by which retinoblastoma protein (Rb) functions in cellular differentiation, we studied primary fibroblasts from the lung buds of wild-type (RB+/+) and null-mutant (RB-/-) mouse embryos. In culture, the RB+/+ fibroblasts differentiated into fat-storing cells, either spontaneously or in response to hormonal induction; otherwise syngenic RB-/- fibroblasts cultured in identical conditions did not. Ectopic expression of normal Rb, but not Rb with a single point mutation, enabled RB-/- fibroblasts to differentiate into adipocytes. Rb appears in murine fibroblasts to activate CCAAT/enhancer-binding proteins (C/EBPs), a family of transcription factors crucial for adipocyte differentiation. Physical interaction between Rb and C/EBPs was demonstrated by reciprocal coimmunoprecipitation, but occurred only in differentiating cells. Wild-type Rb also enhanced the binding of C/EBP to cognate DNA sequences in vitro and the transactivation of a C/EBPbeta-responsive promoter in cells. Taken together, these observations establish a direct and positive role for Rb in terminal differentiation. Such a role contrasts with the function of Rb in arresting cell cycle progression in G1 by negative regulation of other transcription factors like E2F-1.
CiteULike 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  Y. Arima, Y. Inoue, T. Shibata, H. Hayashi, O. Nagano, H. Saya, and Y. Taya Rb Depletion Results in Deregulation of E-Cadherin and Induction of Cellular Phenotypic Changes that Are Characteristic of the Epithelial-to-Mesenchymal Transition Cancer Res., July 1, 2008; 68(13): 5104 - 5112. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Blais, C. J.C. van Oevelen, R. Margueron, D. Acosta-Alvear, and B. D. Dynlacht Retinoblastoma tumor suppressor protein dependent methylation of histone H3 lysine 27 is associated with irreversible cell cycle exit J. Cell Biol., December 31, 2007; 179(7): 1399 - 1412. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Dali-Youcef, C. Mataki, A. Coste, N. Messaddeq, S. Giroud, S. Blanc, C. Koehl, M.-F. Champy, P. Chambon, L. Fajas, et al. Adipose tissue-specific inactivation of the retinoblastoma protein protects against diabesity because of increased energy expenditure PNAS, June 19, 2007; 104(25): 10703 - 10708. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Ahuja, P. Sdek, and W. R. MacLellan Cardiac Myocyte Cell Cycle Control in Development, Disease, and Regeneration Physiol Rev, April 1, 2007; 87(2): 521 - 544. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Takahashi and M. E. Ewen Genetic Interaction between Rb and N-ras: Differentiation Control and Metastasis Cancer Res., October 1, 2006; 66(19): 9345 - 9348. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. J. McFie, G.-L. Wang, N. A. Timchenko, H. L. Wilson, X. Hu, and W. J. Roesler Identification of a Co-repressor That Inhibits the Transcriptional and Growth-Arrest Activities of CCAAT/Enhancer-binding Protein {alpha} J. Biol. Chem., June 30, 2006; 281(26): 18069 - 18080. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. MacWilliams, K. Doquang, R. Pedrola, G. Dollman, D. Grassi, T. Peis, A. Tsang, and A. Ceccarelli A retinoblastoma ortholog controls stalk/spore preference in Dictyostelium Development, April 1, 2006; 133(7): 1287 - 1297. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. B. Goldfine, S. Crunkhorn, M. Costello, H. Gami, E. J. Landaker, M. Niinobe, K. Yoshikawa, D. Lo, A. Warren, J. Jimenez-Chillaron, et al. Necdin and E2F4 Are Modulated by Rosiglitazone Therapy in Diabetic Human Adipose and Muscle Tissue Diabetes, March 1, 2006; 55(3): 640 - 650. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. S. Collins, T. Nakahara, A. Do, and P. F. Lambert Interactions with Pocket Proteins Contribute to the Role of Human Papillomavirus Type 16 E7 in the Papillomavirus Life Cycle J. Virol., December 1, 2005; 79(23): 14769 - 14780. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. F. Johnson Molecular stop signs: regulation of cell-cycle arrest by C/EBP transcription factors J. Cell Sci., June 15, 2005; 118(12): 2545 - 2555. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Batsche, J. Desroches, S. Bilodeau, Y. Gauthier, and J. Drouin Rb Enhances p160/SRC Coactivator-dependent Activity of Nuclear Receptors and Hormone Responsiveness J. Biol. Chem., May 20, 2005; 280(20): 19746 - 19756. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Mantela, Z. Jiang, J. Ylikoski, B. Fritzsch, E. Zacksenhaus, and U. Pirvola The retinoblastoma gene pathway regulates the postmitotic state of hair cells of the mouse inner ear Development, May 15, 2005; 132(10): 2377 - 2388. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Gery, S. Tanosaki, S. Bose, N. Bose, J. Vadgama, and H. P. Koeffler Down-Regulation and Growth Inhibitory Role of C/EBP{alpha} in Breast Cancer Clin. Cancer Res., May 1, 2005; 11(9): 3184 - 3190. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Fu, M. Rao, T. Bouras, C. Wang, K. Wu, X. Zhang, Z. Li, T.-P. Yao, and R. G. Pestell Cyclin D1 Inhibits Peroxisome Proliferator-activated Receptor {gamma}-mediated Adipogenesis through Histone Deacetylase Recruitment J. Biol. Chem., April 29, 2005; 280(17): 16934 - 16941. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. R. MacLellan, A. Garcia, H. Oh, P. Frenkel, M. C. Jordan, K. P. Roos, and M. D. Schneider Overlapping Roles of Pocket Proteins in the Myocardium Are Unmasked by Germ Line Deletion of p130 plus Heart-Specific Deletion of Rb Mol. Cell. Biol., March 15, 2005; 25(6): 2486 - 2497. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G.-L. Wang and N. A. Timchenko Dephosphorylated C/EBP{alpha} Accelerates Cell Proliferation through Sequestering Retinoblastoma Protein Mol. Cell. Biol., February 15, 2005; 25(4): 1325 - 1338. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Qin, X. Li, J.-K. Ko, and N. C. Partridge Parathyroid Hormone Uses Multiple Mechanisms to Arrest the Cell Cycle Progression of Osteoblastic Cells from G1 to S Phase J. Biol. Chem., January 28, 2005; 280(4): 3104 - 3111. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. St-Pierre, X. Liu, L.-C. T. Kha, X. Zhu, O. Ryan, Z. Jiang, and E. Zacksenhaus Conserved and specific functions of mammalian ssu72 Nucleic Acids Res., January 19, 2005; 33(2): 464 - 477. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Tong, J. Tsai, G. Tan, G. Dalgin, and G. S. Hotamisligil Interaction between GATA and the C/EBP Family of Transcription Factors Is Critical in GATA-Mediated Suppression of Adipocyte Differentiation Mol. Cell. Biol., January 15, 2005; 25(2): 706 - 715. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Takahashi, B. Contreras, R. T. Bronson, M. Loda, and M. E. Ewen Genetic Interaction between Rb and K-ras in the Control of Differentiation and Tumor Suppression Mol. Cell. Biol., December 1, 2004; 24(23): 10406 - 10415. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. A. Wikenheiser-Brokamp Rb family proteins differentially regulate distinct cell lineages during epithelial development Development, September 1, 2004; 131(17): 4299 - 4310. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Prisco, A. Maiorana, C. Guerzoni, G. Calin, B. Calabretta, R. Voit, I. Grummt, and R. Baserga Role of Pescadillo and Upstream Binding Factor in the Proliferation and Differentiation of Murine Myeloid Cells Mol. Cell. Biol., June 15, 2004; 24(12): 5421 - 5433. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Ruiz, M. Santos, C. Segrelles, H. Leis, J. L. Jorcano, A. Berns, J. M. Paramio, and M. Vooijs Unique and overlapping functions of pRb and p107 in the control of proliferation and differentiation in epidermis Development, June 1, 2004; 131(11): 2737 - 2748. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Pennaneach, V. Barbier, K. Regazzoni, R. Fotedar, and A. Fotedar Rb Inhibits E2F-1-induced Cell Death in a LXCXE-dependent Manner by Active Repression J. Biol. Chem., May 28, 2004; 279(22): 23376 - 23383. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Felipe, M. L. Bonet, J. Ribot, and A. Palou Modulation of Resistin Expression by Retinoic Acid and Vitamin A Status Diabetes, April 1, 2004; 53(4): 882 - 889. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. B. Hansen, C. Jorgensen, R. K. Petersen, P. Hallenborg, R. De Matteis, H. A. Boye, N. Petrovic, S. Enerback, J. Nedergaard, S. Cinti, et al. Retinoblastoma protein functions as a molecular switch determining white versus brown adipocyte differentiation PNAS, March 23, 2004; 101(12): 4112 - 4117. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Muller, C. F. Calkhoven, X. Sha, and A. Leutz The CCAAT Enhancer-binding Protein {alpha} (C/EBP{alpha}) Requires a SWI/SNF Complex for Proliferation Arrest J. Biol. Chem., February 20, 2004; 279(8): 7353 - 7358. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. A. Cole, A. W. Harmon, J. B. Harp, and Y. M. Patel Rb regulates C/EBP{beta}-DNA-binding activity during 3T3-L1 adipogenesis Am J Physiol Cell Physiol, February 1, 2004; 286(2): C349 - C354. [Abstract] [Full Text]
|
|
|
|
 |
|
 S. Gery, A. F. Gombart, Y. K. Fung, and H. P. Koeffler C/EBP{epsilon} interacts with retinoblastoma and E2F1 during granulopoiesis Blood, February 1, 2004; 103(3): 828 - 835. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. E. Ross, H. S. Radomska, B. Wu, P. Zhang, J. N. Winnay, L. Bajnok, W. S. Wright, F. Schaufele, D. G. Tenen, and O. A. MacDougald Phosphorylation of C/EBP{alpha} Inhibits Granulopoiesis Mol. Cell. Biol., January 15, 2004; 24(2): 675 - 686. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. M. Pierantoni, S. Battista, F. Pentimalli, M. Fedele, R. Visone, A. Federico, M. Santoro, G. Viglietto, and A. Fusco A truncated HMGA1 gene induces proliferation of the 3T3-L1 pre-adipocytic cells: a model of human lipomas Carcinogenesis, December 1, 2003; 24(12): 1861 - 1869. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. D'Alo', L. M. Johansen, E. A. Nelson, H. S. Radomska, E. K. Evans, P. Zhang, C. Nerlov, and D. G. Tenen The amino terminal and E2F interaction domains are critical for C/EBP{alpha}-mediated induction of granulopoietic development of hematopoietic cells Blood, November 1, 2003; 102(9): 3163 - 3171. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Takahashi, R. T. Bronson, M. Socolovsky, B. Contreras, K. Y. Lee, T. Jacks, M. Noda, R. Kucherlapati, and M. E. Ewen Rb and N-ras Function Together To Control Differentiation in the Mouse Mol. Cell. Biol., August 1, 2003; 23(15): 5256 - 5268. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. S. Fay, E. Large, M. Han, and M. Darland lin-35/Rb and ubc-18, an E2 ubiquitin-conjugating enzyme, function redundantly to control pharyngeal morphogenesis in C. elegans Development, July 15, 2003; 130(14): 3319 - 3330. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Ruiz, C. Segrelles, A. Bravo, M. Santos, P. Perez, H. Leis, J. L. Jorcano, and J. M. Paramio Abnormal epidermal differentiation and impaired epithelial-mesenchymal tissue interactions in mice lacking the retinoblastoma relatives p107 and p130 Development, June 1, 2003; 130(11): 2341 - 2353. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. de Bruin, L. Wu, H. I. Saavedra, P. Wilson, Y. Yang, T. J. Rosol, M. Weinstein, M. L. Robinson, and G. Leone Rb function in extraembryonic lineages suppresses apoptosis in the CNS of Rb-deficient mice PNAS, May 27, 2003; 100(11): 6546 - 6551. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Shim and R. C. Smart Lithium Stabilizes the CCAAT/Enhancer-binding Protein {alpha} (C/EBP{alpha}) through a Glycogen Synthase Kinase 3 (GSK3)-independent Pathway Involving Direct Inhibition of Proteasomal Activity J. Biol. Chem., May 23, 2003; 278(22): 19674 - 19681. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Takebayashi, H. Higashi, H. Sudo, H. Ozawa, E. Suzuki, O. Shirado, H. Katoh, and M. Hatakeyama NF-kappa B-dependent Induction of Cyclin D1 by Retinoblastoma Protein (pRB) Family Proteins and Tumor-derived pRB Mutants J. Biol. Chem., April 18, 2003; 278(17): 14897 - 14905. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. L. Landsberg, J. E. Sero, P. S. Danielian, T. L. Yuan, E. Y. Lee, and J. A. Lees The role of E2F4 in adipogenesis is independent of its cell cycle regulatory activity PNAS, March 4, 2003; 100(5): 2456 - 2461. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z.D. Sharp, W.-H. Lee, A. Y. Nikitin, A. Flesken-Nikitin, Y. Ikeno, R. Reddick, A. G. Richardson, and J. F. Nelson Minimal effects of dietary restriction on neuroendocrine carcinogenesis in Rb+/- mice Carcinogenesis, February 1, 2003; 24(2): 179 - 183. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Decary, J. T. Decesse, V. Ogryzko, J. C. Reed, I. Naguibneva, A. Harel-Bellan, and C. E. Cremisi The Retinoblastoma Protein Binds the Promoter of the Survival Gene bcl-2 and Regulates Its Transcription in Epithelial Cells through Transcription Factor AP-2 Mol. Cell. Biol., November 15, 2002; 22(22): 7877 - 7888. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Feliers, M. A. Frank, and D. J. Riley Activation of Cyclin D1-Cdk4 and Cdk4-Directed Phosphorylation of RB Protein in Diabetic Mesangial Hypertrophy Diabetes, November 1, 2002; 51(11): 3290 - 3299. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. B. Hansen, R. K. Petersen, C. Jorgensen, and K. Kristiansen Deregulated MAPK Activity Prevents Adipocyte Differentiation of Fibroblasts Lacking the Retinoblastoma Protein J. Biol. Chem., July 12, 2002; 277(29): 26335 - 26339. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. P. BOTTARO, A. LIEBMANN-VINSON, and M. A. HEIDARAN Molecular Signaling in Bioengineered Tissue Microenvironments Ann. N.Y. Acad. Sci., June 1, 2002; 961(1): 143 - 153. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. D. Brown and B. L. Gallie The B-Domain Lysine Patch of pRB Is Required for Binding to Large T Antigen and Release of E2F by Phosphorylation Mol. Cell. Biol., March 1, 2002; 22(5): 1390 - 1401. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. M. Johansen, A. Iwama, T. A. Lodie, K. Sasaki, D. W. Felsher, T. R. Golub, and D. G. Tenen c-Myc Is a Critical Target for C/EBP{alpha} in Granulopoiesis Mol. Cell. Biol., June 1, 2001; 21(11): 3789 - 3806. [Abstract] [Full Text]
|
|
|
|
|
|
R. M. Melillo, G. M. Pierantoni, S. Scala, S. Battista, M. Fedele, A. Stella, M. C. De Biasio, G. Chiappetta, V. Fidanza, G. Condorelli, et al. Critical Role of the HMGI(Y) Proteins in Adipocytic Cell Growth and Differentiation Mol. Cell. Biol., April 1, 2001; 21(7): 2485 - 2495. [Abstract] [Full Text]
|
|
|
|
|
|
A. Yu. Nikitin, B. Shan, A. Flesken-Nikitin, K.-H. Chang, and W.-H. Lee The Retinoblastoma Gene Regulates Somatic Growth during Mouse Development Cancer Res., April 1, 2001; 61(7): 3110 - 3118. [Abstract] [Full Text]
|
|
|
|
 |
|
 H. Müller, A. P. Bracken, R. Vernell, M. C. Moroni, F. Christians, E. Grassilli, E. Prosperini, E. Vigo, J. D. Oliner, and K. Helin E2Fs regulate the expression of genes involved in differentiation, development, proliferation, and apoptosis Genes & Dev., February 1, 2001; 15(3): 267 - 285. [Abstract] [Full Text]
|
|
|
|
|
|
S. Shimba, T. Wada, and M. Tezuka Arylhydrocarbon receptor (AhR) is involved in negative regulation of adipose differentiation in 3T3-L1 cells: AhR inhibits adipose differentiation independently of dioxin J. Cell Sci., January 8, 2001; 114(15): 2809 - 2817. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. A. Hirsch, L. Gu, and R. W. Henry The Retinoblastoma Tumor Suppressor Protein Targets Distinct General Transcription Factors To Regulate RNA Polymerase III Gene Expression Mol. Cell. Biol., December 15, 2000; 20(24): 9182 - 9191. [Abstract] [Full Text]
|
|
|
|
|
|
S. Miyake, W. R. Sellers, M. Safran, X. Li, W. Zhao, S. R. Grossman, J. Gan, J. A. DeCaprio, P. D. Adams, and W. G. Kaelin Jr. Cells Degrade a Novel Inhibitor of Differentiation with E1A-Like Properties upon Exiting the Cell Cycle Mol. Cell. Biol., December 1, 2000; 20(23): 8889 - 8902. [Abstract] [Full Text]
|
|
|
|
|
|
W. R. MacLellan, G. Xiao, M. Abdellatif, and M. D. Schneider A Novel Rb- and p300-Binding Protein Inhibits Transactivation by MyoD Mol. Cell. Biol., December 1, 2000; 20(23): 8903 - 8915. [Abstract] [Full Text]
|
|
|
|
|
|
M. Classon, B. K. Kennedy, R. Mulloy, and E. Harlow Opposing roles of pRB and p107 in adipocyte differentiation PNAS, September 19, 2000; (2000) 190343597. [Abstract] [Full Text]
|
|
|
|
|
|
C. Lemercier, K. Duncliffe, I. Boibessot, H. Zhang, A. Verdel, D. Angelov, and S. Khochbin Involvement of Retinoblastoma Protein and HBP1 in Histone H10 Gene Expression Mol. Cell. Biol., September 15, 2000; 20(18): 6627 - 6637. [Abstract] [Full Text]
|
|
|
|
|
|
B. A. Slomiany, K. L. D'Arigo, M. M. Kelly, and D. T. Kurtz C/EBPalpha Inhibits Cell Growth via Direct Repression of E2F-DP-Mediated Transcription Mol. Cell. Biol., August 15, 2000; 20(16): 5986 - 5997. [Abstract] [Full Text]
|
|
|
|
|
|
L. Vitelli, G. Condorelli, V. Lulli, T. Hoang, L. Luchetti, C. M. Croce, and C. Peschle A Pentamer Transcriptional Complex Including tal-1 and Retinoblastoma Protein Downmodulates c-kit Expression in Normal Erythroblasts Mol. Cell. Biol., July 15, 2000; 20(14): 5330 - 5342. [Abstract] [Full Text]
|
|
|
|
|
|
L. Fajas, C. Paul, O. Zugasti, L. Le Cam, J. Polanowska, E. Fabbrizio, R. Medema, M.-L. Vignais, and C. Sardet pRB binds to and modulates the transrepressing activity of the E1A-regulated transcription factor p120E4F PNAS, June 23, 2000; (2000) 130198397. [Abstract] [Full Text] |
