![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Research Papers
Friedrich Miescher Institute, Basel, Switzerland.
Abstract
The LIM motif defines a double zinc finger structure found in proteins involved in cell fate determination and growth control. LIM proteins, which include LIM homeo domain, LIM kinase, focal adhesion, and LIM-only proteins, usually contain two or more LIM motifs clustered at their amino- or carboxy-terminal end. At present, the mode of action of the LIM domain is not clear. In this study we have analyzed the binding properties of LIM motifs in the cellular environment. We show that MLP, CRP, and betaCRP define a subclass of LIM-only proteins with unique dual subcellular localization in the nucleus and along actin-based filaments in the cytosol. A double MLP construct that accumulated nearly exclusively along actin filaments promoted myogenic differentiation efficiently, arguing for a functional role of cytoskeleton-associated MLP. Binding of MLP to the actin cytoskeleton is specifically attributable to its second LIM motif. An additional LIM motif potentiates binding. Potentiating LIM motifs can be interchanged, resulting in differential targeting of interacting proteins. To analyze LIM-LIM interactions in situ, this property was exploited to develop a hybrid interaction approach based on the relocalization of LIM-containing constructs to the actin cytoskeleton. These experiments revealed the existence of marked selectivity in the interactions of single LIM motifs, and among LIM domains from different LIM-homeo domain and LIM-only proteins. Furthermore, the analysis suggested that the LIM motif has two interacting interfaces. On the basis of these findings, we propose that LIM motifs function as specific adapter elements to promote the assembly and targeting of multiprotein complexes.
CiteULike 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  H.-J. Wang, A.-R. Wan, and G.-Y. Jauh An Actin-Binding Protein, LlLIM1, Mediates Calcium and Hydrogen Regulation of Actin Dynamics in Pollen Tubes Plant Physiology, August 1, 2008; 147(4): 1619 - 1636. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D.-W. Lin, I.-C. Chang, A. Tseng, M.-L. Wu, C.-H. Chen, C. A. Patenaude, M. D. Layne, and S.-F. Yet Transforming Growth Factor {beta} Up-regulates Cysteine-rich Protein 2 in Vascular Smooth Muscle Cells via Activating Transcription Factor 2 J. Biol. Chem., May 30, 2008; 283(22): 15003 - 15014. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Thomas, F. Moreau, M. Dieterle, C. Hoffmann, S. Gatti, C. Hofmann, M. Van Troys, C. Ampe, and A. Steinmetz The LIM Domains of WLIM1 Define a New Class of Actin Bundling Modules J. Biol. Chem., November 16, 2007; 282(46): 33599 - 33608. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. C. Kudo, S. L. Karsten, J. Chen, P. Levitt, and D. H. Geschwind Genetic Analysis of Anterior Posterior Expression Gradients in the Developing Mammalian Forebrain Cereb Cortex, September 1, 2007; 17(9): 2108 - 2122. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Y. Boateng, R. J. Belin, D. L. Geenen, K. B. Margulies, J. L. Martin, M. Hoshijima, P. P. de Tombe, and B. Russell Cardiac dysfunction and heart failure are associated with abnormalities in the subcellular distribution and amounts of oligomeric muscle LIM protein Am J Physiol Heart Circ Physiol, January 1, 2007; 292(1): H259 - H269. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Thomas, C. Hoffmann, M. Dieterle, M. Van Troys, C. Ampe, and A. Steinmetz Tobacco WLIM1 Is a Novel F-Actin Binding Protein Involved in Actin Cytoskeleton Remodeling PLANT CELL, September 1, 2006; 18(9): 2194 - 2206. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Hoshijima Mechanical stress-strain sensors embedded in cardiac cytoskeleton: Z disk, titin, and associated structures Am J Physiol Heart Circ Physiol, April 1, 2006; 290(4): H1313 - H1325. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Wei, T. E. Gorman, X. Liu, B. Ith, A. Tseng, Z. Chen, D. I. Simon, M. D. Layne, and S.-F. Yet Increased Neointima Formation in Cysteine-Rich Protein 2-Deficient Mice in Response to Vascular Injury Circ. Res., December 9, 2005; 97(12): 1323 - 1331. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Novotny-Diermayr, B. Lin, L. Gu, and X. Cao Modulation of the Interleukin-6 Receptor Subunit Glycoprotein 130 Complex and Its Signaling by LMO4 Interaction J. Biol. Chem., April 1, 2005; 280(13): 12747 - 12757. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Heineke, H. Ruetten, C. Willenbockel, S. C. Gross, M. Naguib, A. Schaefer, T. Kempf, D. Hilfiker-Kleiner, P. Caroni, T. Kraft, et al. Attenuation of cardiac remodeling after myocardial infarction by muscle LIM protein-calcineurin signaling at the sarcomeric Z-disc PNAS, February 1, 2005; 102(5): 1655 - 1660. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. C. Brown and C. E. Turner Paxillin: Adapting to Change Physiol Rev, October 1, 2004; 84(4): 1315 - 1339. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. I. Pueyo and J. P. Couso Chip-mediated partnerships of the homeodomain proteins Bar and Aristaless with the LIM-HOM proteins Apterous and Lim1 regulate distal leg development Development, July 1, 2004; 131(13): 3107 - 3120. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Lahlil, E. Lecuyer, S. Herblot, and T. Hoang SCL Assembles a Multifactorial Complex That Determines Glycophorin A Expression Mol. Cell. Biol., February 15, 2004; 24(4): 1439 - 1452. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Akazawa, S. Kudoh, N. Mochizuki, N. Takekoshi, H. Takano, T. Nagai, and I. Komuro A novel LIM protein Cal promotes cardiac differentiation by association with CSX/NKX2-5 J. Cell Biol., February 2, 2004; 164(3): 395 - 405. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Shimojo and L. B. Hersh REST/NRSF-Interacting LIM Domain Protein, a Putative Nuclear Translocation Receptor Mol. Cell. Biol., December 15, 2003; 23(24): 9025 - 9031. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Briere, A.-C. Bordel, H. Barthou, A. Jauneau, A. Steinmetz, G. Alibert, and M. Petitprez Is the LIM-domain Protein HaWLIM1 Associated with Cortical Microtubules in Sunflower Protoplasts? Plant Cell Physiol., October 15, 2003; 44(10): 1055 - 1063. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-F. Chang, J. Wei, X. Liu, Y.-H. Chen, M. D. Layne, and S.-F. Yet Identification of a CArG-independent region of the cysteine-rich protein 2 promoter that directs expression in the developing vasculature Am J Physiol Heart Circ Physiol, October 1, 2003; 285(4): H1675 - H1683. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. J. Lips, L. J. deWindt, D. J.W. van Kraaij, and P. A. Doevendans Molecular determinants of myocardial hypertrophy and failure: alternative pathways for beneficial and maladaptive hypertrophy Eur. Heart J., May 2, 2003; 24(10): 883 - 896. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Heineke, T. Kempf, T. Kraft, A. Hilfiker, H. Morawietz, R. J. Scheubel, P. Caroni, S. M. Lohmann, H. Drexler, and K. C. Wollert Downregulation of Cytoskeletal Muscle LIM Protein by Nitric Oxide: Impact on Cardiac Myocyte Hypertrophy Circulation, March 18, 2003; 107(10): 1424 - 1432. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. S. Coutts, E. MacKenzie, E. Griffith, and D. M. Black TES is a novel focal adhesion protein with a role in cell spreading J. Cell Sci., March 1, 2003; 116(5): 897 - 906. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Kirchner, K. A. Forbush, and M. J. Bevan Identification and Characterization of Thymus LIM Protein: Targeted Disruption Reduces Thymus Cellularity Mol. Cell. Biol., December 15, 2001; 21(24): 8592 - 8604. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Ehler, R. Horowits, C. Zuppinger, R. L. Price, E. Perriard, M. Leu, P. Caroni, M. Sussman, H. M. Eppenberger, and J.-C. Perriard Alterations at the Intercalated Disk Associated with the Absence of Muscle LIM Protein J. Cell Biol., May 14, 2001; 153(4): 763 - 772. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Yang, C. S. Moravec, M. A. Sussman, N. R. DiPaola, D. Fu, L. Hawthorn, C. A. Mitchell, J. B. Young, G. S. Francis, P. M. McCarthy, et al. Decreased SLIM1 Expression and Increased Gelsolin Expression in Failing Human Hearts Measured by High-Density Oligonucleotide Arrays Circulation, December 19, 2000; 102(25): 3046 - 3052. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P.-H. Chu, W. M. Bardwell, Y. Gu, J. Ross Jr., and J. Chen FHL2 (SLIM3) Is Not Essential for Cardiac Development and Function Mol. Cell. Biol., October 15, 2000; 20(20): 7460 - 7462. [Abstract] [Full Text]
|
|
|
|
 |
|
 F. Gay, I. Anglade, Z. Gong, and G. Salbert The LIM/Homeodomain Protein Islet-1 Modulates Estrogen Receptor Functions Mol. Endocrinol., October 1, 2000; 14(10): 1627 - 1648. [Abstract] [Full Text]
|
|
|
|
|
|
A. M. Katz Cytoskeletal Abnormalities in the Failing Heart : Out on a LIM? Circulation, June 13, 2000; 101(23): 2672 - 2673. [Full Text] [PDF]
|
|
|
|
|
|
O. Zolk, P. Caroni, and M. Bohm Decreased Expression of the Cardiac LIM Domain Protein MLP in Chronic Human Heart Failure Circulation, June 13, 2000; 101(23): 2674 - 2677. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. van Meyel, D. O'Keefe, S Thor, L. Jurata, G. Gill, and J. Thomas Chip is an essential cofactor for apterous in the regulation of axon guidance in Drosophila Development, January 5, 2000; 127(9): 1823 - 1831. [Abstract] [PDF]
|
|
|
|
|
|
D. J. Glenn and R. A. Maurer MRG1 Binds to the LIM Domain of Lhx2 and May Function as a Coactivator to Stimulate Glycoprotein Hormone alpha -Subunit Gene Expression J. Biol. Chem., December 17, 1999; 274(51): 36159 - 36167. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Brown, M. J. McGrath, L. M. Ooms, R. Gurung, M. M. Maimone, and C. A. Mitchell Characterization of Two Isoforms of the Skeletal Muscle LIM Protein 1, SLIM1. LOCALIZATION OF SLIM1 AT FOCAL ADHESIONS AND THE ISOFORM SLIMMER IN THE NUCLEUS OF MYOBLASTS AND CYTOPLASM OF MYOTUBES SUGGESTS DISTINCT ROLES IN THE CYTOSKELETON AND IN NUCLEAR-CYTOPLASMIC COMMUNICATION J. Biol. Chem., September 17, 1999; 274(38): 27083 - 27091. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. E. Stronach, P. J. Renfranz, B. Lilly, and M. C. Beckerle Muscle LIM Proteins Are Associated with Muscle Sarcomeres and Require dMEF2 for Their Expression during Drosophila Myogenesis Mol. Biol. Cell, July 1, 1999; 10(7): 2329 - 2342. [Abstract] [Full Text]
|
|
|
|
|
|
P. M. Guy, D. A. Kenny, and G. N. Gill The PDZ Domain of the LIM Protein Enigma Binds to beta -Tropomyosin Mol. Biol. Cell, June 1, 1999; 10(6): 1973 - 1984. [Abstract] [Full Text]
|
|
|
|
 |
|
 A. G. Schneider, K. R. Sultan, and D. Pette Muscle LIM protein: expressed in slow muscle and induced in fast muscle by enhanced contractile activity Am J Physiol Cell Physiol, April 1, 1999; 276(4): C900 - C906. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Hobert, D. G. Moerman, K. A. Clark, M. C. Beckerle, and G. Ruvkun A Conserved LIM Protein That Affects Muscular Adherens Junction Integrity and Mechanosensory Function in Caenorhabditis elegans J. Cell Biol., January 11, 1999; 144(1): 45 - 57. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Thomas, M Hagel, and C. Turner Characterization of a focal adhesion protein, Hic-5, that shares extensive homology with paxillin J. Cell Sci., January 1, 1999; 112(2): 181 - 190. [Abstract] [PDF]
|
|
|
|
|
|
M.-F. Champliaud, R. E. Burgeson, W. Jin, H. P. Baden, and P. F. Olson cDNA Cloning and Characterization of Sciellin, a LIM Domain Protein of the Keratinocyte Cornified Envelope J. Biol. Chem., November 20, 1998; 273(47): 31547 - 31554. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. K. Jain, S. Kashiki, C.-M. Hsieh, M. D. Layne, S.-F. Yet, N. E. S. Sibinga, M. T. Chin, M. W. Feinberg, I. Woo, R. L. Maas, et al. Embryonic Expression Suggests an Important Role for CRP2/SmLIM in the Developing Cardiovascular System Circ. Res., November 16, 1998; 83(10): 980 - 985. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Konrat, B. Krautler, R. Weiskirchen, and K. Bister Structure of Cysteine- and Glycine-rich Protein CRP2. BACKBONE DYNAMICS REVEAL MOTIONAL FREEDOM AND INDEPENDENT SPATIAL ORIENTATION OF THE LIM DOMAINS J. Biol. Chem., September 4, 1998; 273(36): 23233 - 23240. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Zeng, N. J. Justice, S. Abdelilah, Y.-M. Chan, L. Y. Jan, and Y. N. Jan The Drosophila LIM-only gene, dLMO, is mutated in Beadex alleles and might represent an evolutionarily conserved function in appendage development PNAS, September 1, 1998; 95(18): 10637 - 10642. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Shoresh, S. Orgad, O. Shmueli, R. Werczberger, D. Gelbaum, S. Abiri, and D. Segal Overexpression Beadex Mutations and Loss-of-Function heldup-a Mutations in Drosophila Affect the 3' Regulatory and Coding Components, Respectively, of the Dlmo Gene Genetics, September 1, 1998; 150(1): 283 - 299. [Abstract] [Full Text]
|
|
|
|
|
|
S. M. Ahern-Djamali, A. R. Comer, C. Bachmann, A. S. Kastenmeier, S. K. Reddy, M. C. Beckerle, U. Walter, and F. M. Hoffmann Mutations in Drosophila Enabled and Rescue by Human Vasodilator-stimulated Phosphoprotein (VASP) Indicate Important Functional Roles for Ena/VASP Homology Domain 1 (EVH1) and EVH2 Domains Mol. Biol. Cell, August 1, 1998; 9(8): 2157 - 2171. [Abstract] [Full Text]
|
|
|
|
|
|
C. S. Chew, J. A. Parente Jr., C.-J. Zhou, E. Baranco, and X. Chen Lasp-1 is a regulated phosphoprotein within the cAMP signaling pathway in the gastric parietal cell Am J Physiol Cell Physiol, July 1, 1998; 275(1): C56 - C67. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. C. Brown, J. A. Perrotta, and C. E. Turner Serine and Threonine Phosphorylation of the Paxillin LIM Domains Regulates Paxillin Focal Adhesion Localization and Cell Adhesion to Fibronectin Mol. Biol. Cell, July 1, 1998; 9(7): 1803 - 1816. [Abstract] [Full Text]
|
|
|
|
|
|
S.-F. Yet, S. C. Folta, M. K. Jain, C.-M. Hsieh, K. Maemura, M. D. Layne, D. Zhang, P. B. Marria, M. Yoshizumi, M. T. Chin, et al. Molecular Cloning, Characterization, and Promoter Analysis of the Mouse Crp2/SmLim Gene. PREFERENTIAL EXPRESSION OF ITS PROMOTER IN THE VASCULAR SMOOTH MUSCLE CELLS OF TRANSGENIC MICE J. Biol. Chem., April 24, 1998; 273(17): 10530 - 10537. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Prassler, A. Murr, S. Stocker, J. Faix, J. Murphy, and G. Marriott DdLIM Is a Cytoskeleton-associated Protein Involved in the Protrusion of Lamellipodia in Dictyostelium Mol. Biol. Cell, March 1, 1998; 9(3): 545 - 559. [Abstract] [Full Text]
|
|
|
|
|
|
E. Cuppen, H. Gerrits, B. Pepers, B. Wieringa, and W. Hendriks PDZ Motifs in PTP-BL and RIL Bind to Internal Protein Segments in the LIM Domain Protein RIL Mol. Biol. Cell, March 1, 1998; 9(3): 671 - 683. [Abstract] [Full Text]
|
|
|
|
|
|
J. J. Breen, A. D. Agulnick, H. Westphal, and I. B. Dawid Interactions between LIM Domains and the LIM Domain-binding Protein Ldb1 J. Biol. Chem., February 20, 1998; 273(8): 4712 - 4717. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. W. Jurata, S. L. Pfaff, and G. N. Gill The Nuclear LIM Domain Interactor NLI Mediates Homo- and Heterodimerization of LIM Domain Transcription Factors J. Biol. Chem., February 6, 1998; 273(6): 3152 - 3157. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. O'Keefe, S Thor, and J. Thomas Function and specificity of LIM domains in Drosophila nervous system and wing development Development, January 10, 1998; 125(19): 3915 - 3923. [Abstract] [PDF]
|
|
|
|
|
|
M Grigoriou, A. Tucker, P. Sharpe, and V Pachnis Expression and regulation of Lhx6 and Lhx7, a novel subfamily of LIM homeodomain encoding genes, suggests a role in mammalian head development Development, January 6, 1998; 125(11): 2063 - 2074. [Abstract] [PDF]
|
|
|
|
|
|
H. A. Louis, J. D. Pino, K. L. Schmeichel, P. Pomies, and M. C. Beckerle Comparison of Three Members of the Cysteine-rich Protein Family Reveals Functional Conservation and Divergent Patterns of Gene Expression J. Biol. Chem., October 24, 1997; 272(43): 27484 - 27491. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Pomies, H. A. Louis, and M. C. Beckerle CRP1, a LIM Domain Protein Implicated in Muscle Differentiation, Interacts with alpha -Actinin J. Cell Biol., October 6, 1997; 139(1): 157 - 168. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. J. Roof, A. Hayes, M. Adamian, A. H. Chishti, and T. Li Molecular Characterization of abLIM, a Novel Actin-binding and Double Zinc Finger Protein J. Cell Biol., August 11, 1997; 138(3): 575 - 588. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Konrat, R. Weiskirchen, B. Krautler, and K. Bister Solution Structure of the Carboxyl-terminal LIM Domain from Quail Cysteine-rich Protein CRP2 J. Biol. Chem., May 2, 1997; 272(18): 12001 - 12007. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Nahm, S. K. Woo, J. S. Handler, and H. M. Kwon Involvement of multiple kinase pathways in stimulation of gene transcription by hypertonicity Am J Physiol Cell Physiol, January 1, 2002; 282(1): C49 - C58. [Abstract] [Full Text] [PDF]
|
|
