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Genes and Development
Vol. 11, No. 17,
pp. 2272-2290,
September 1, 1997
a yeast mutation that blocks double-strand-break
processing and permits nonhomologous synapsis in meiosis
Institut für Botanik, Abteilung für Zytologie und
Genetik, 1030 Vienna, Austria
During meiotic prophase the repair of self-inflicted DNA
double-strand break (DSB) damage leads to meiotic recombination in yeast. We employed a genetic screen to specifically characterize cellular functions that become essential after this DSB formation. As a
result a new allele of MRE11, termed mre11S (for
Separation of functions) was isolated that allows
initiation but not processing and repair of meiotic DSBs similar to the
well-characterized rad50S allele. In contrast, the
mre11-1 allele blocks initiation of meiotic DSBs as reported
previously by others. The mre11S allele, which is mutated in
the 5
part of the gene, can partially complement mre11
alleles disrupted close to the 3 end that cannot initiate DSBs
when homozygous. This suggests homodimerization of the Mre11 protein
and the presence of separate domains for DSB initiation and 5
resection. The fact that two genes, RAD50 and MRE11,
required for DSB processing are also essential for DSB initiation
dictates a model in which a bifunctional
initiation/repair complex is required to initiate meiotic
recombination. A subset of mre11S nuclei was shown to perform
extensive but partially nonhomologous synapsis. We propose that the
unprocessed DSBs present in mre11S allow for synapsis, but that
homologous synapsis is only ensured at a later stage of recombination.
[Key Words: Meiosis; synaptonemal complex; MRE11; recombination; DNA double-strand breaks; Saccharomyces cerevisiae]
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