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1 Department of Cell Biology, Yale University School of
Medicine, New Haven, Connecticut 06510 USA;
2 Department of
Biology, Yale University, New Haven, Connecticut 06520 USA
The gene for an essential protein subunit of nuclear RNase P from
Saccharomyces cerevisiae has been cloned. The gene for this protein, RPP1, was identified by virtue of its homology with a human scleroderma autoimmune antigen, Rpp30, which copurifies with
human RNase P. Epitope-tagged Rpp1 can be found in association with
both RNase P RNA and a related endoribonuclease, RNase MRP RNA, in
immunoprecipitates from crude extracts of cells. Depletion of Rpp1 in
vivo leads to the accumulation of precursor tRNAs with unprocessed
5
and 3 termini and reveals rRNA processing defects that have
not been described previously for proteins associated with RNase P or
RNase MRP. Immunoprecipitated complexes cleave both yeast precursor
tRNAs and precursor rRNAs.
[Key Words: Rpp1; essential protein subunit; nuclear RNase P; S. cerevisiae; precursor tRNA; precursor rRNA]
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