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Genes and Development
Vol. 11, No. 19,
pp. 2494-2509,
October 1, 1997
-globin locus
1 Sir William Dunn School of Pathology, University of
Oxford, Oxford OX1 3RE, UK;
2 Erasmus University Rotterdam,
Medical Genetics Center Department of Cell Biology and Genetics,
3000 DR Rotterdam, The Netherlands
We have identified novel nuclear transcripts in the human
-globin locus using nuclear run-on analysis in erythroid cell lines and in situ hybridization analysis of erythroid tissue. These transcripts extend across the LCR and intergenic regions but are undetectable in nonerythroid cells. Surprisingly, transient
transfection of a -globin gene (
, 
, or ) induces
transcription of the LCR and intergenic regions from the chromosomal
-globin locus in nonerythroid cell lines. The -globin genes
themselves, however, remain transcriptionally silent. Induction is
dependent on transcription of the globin gene in the transfected
plasmid but does not require protein expression. Using in situ
hybridization analysis, we show that the plasmid colocalizes with the
endogenous -globin locus providing insight into the mechanism of
transinduction.
[Key Words:
Human -globin locus; transcription; transinduction; locus control region]
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|
|
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|
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|
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|
|
|
|
 |
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|
|
|
|
|
|
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|
|
|
|
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|
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|
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|
|
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|
|
|
|
|
|
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|
|
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|
|
|
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|
|
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|
 |
|
 J. S. Mattick and M. J. Gagen Review ArticleThe Evolution of Controlled Multitasked Gene Networks: The Role of Introns and Other Noncoding RNAs in the Development of Complex Organisms Mol. Biol. Evol., September 1, 2001; 18(9): 1611 - 1630. [Abstract] [Full Text] [PDF]
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