![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Howard Hughes Medical Institute, Carnegie Institution of
Washington, Department of Embryology, Baltimore, Maryland 21210 USA
The centromere-kinetochore complex is a specialized chromatin
structure that mediates bipolar attachment of replicated chromosomes to
the mitotic spindle, thereby ensuring proper sister chromatid separation during anaphase. The manner in which this important multimeric structure is specified and assembled within chromatin is
unknown. Using in vivo cross-linking followed by immunoprecipitation, we show that the Mif2 protein of the budding yeast Saccharomyces cerevisiae, previously implicated in centromere function by genetic criteria, resides specifically at centromeric loci in vivo. This provides definitive evidence for structural conservation between yeast
and mammalian centromeres, as Mif2p shares homology with CENP-C, a
mammalian centromere protein. Ndc10p and Cbf1p, previously implicated
in centromere function by genetic and in vitro biochemical assays, were
also found to interact with centromeric DNA in vivo. By examining
Mif2p, Ndc10p, and Cbf1p association with centromeric DNA derivatives,
we demonstrate the existence of centromeric subcomplexes that may
correspond to assembly intermediates. Based on these observations, we
provide a simple model for centromere assembly. Finally, given the
sensitivity of this technique, its application to other
sequence-specific protein-DNA complexes within the cell, such as
origins of replication and enhancer-promoter regions, could be of
significant value.
[Key Words: MIF2; CENP-C; centromere; kinetochore; chromatin immunoprecipitation; CSE4]
CiteULike 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  S. Vernarecci, P. Ornaghi, A. Bagu, E. Cundari, P. Ballario, and P. Filetici Gcn5p Plays an Important Role in Centromere Kinetochore Function in Budding Yeast Mol. Cell. Biol., February 1, 2008; 28(3): 988 - 996. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Bausch, S. Noone, J. M. Henry, K. Gaudenz, B. Sanderson, C. Seidel, and J. L. Gerton Transcription Alters Chromosomal Locations of Cohesin in Saccharomyces cerevisiae Mol. Cell. Biol., December 15, 2007; 27(24): 8522 - 8532. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Wong, Y. Nakajima, S. Westermann, C. Shang, J.-s. Kang, C. Goodner, P. Houshmand, S. Fields, C. S.M. Chan, D. Drubin, et al. A Protein Interaction Map of the Mitotic Spindle Mol. Biol. Cell, October 1, 2007; 18(10): 3800 - 3809. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. W. Lam, E. A. Peterson, M. Yeung, and B. D. Lavoie Condensin is required for chromosome arm cohesion during mitosis. Genes & Dev., November 1, 2006; 20(21): 2973 - 2984. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Kanta, L. Laprade, A. Almutairi, and I. Pinto Suppressor Analysis of a Histone Defect Identifies a New Function for the Hda1 Complex in Chromosome Segregation Genetics, May 1, 2006; 173(1): 435 - 450. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Powell The kinetochore uncoiled J. Cell Biol., April 10, 2006; 173(1): 7 - 7. [Full Text] [PDF]
|
|
|
|
|
|
R. E. Baker and K. Rogers Genetic and Genomic Analysis of the AT-Rich Centromere DNA Element II of Saccharomyces cerevisiae Genetics, December 1, 2005; 171(4): 1463 - 1475. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Stoepel, M. A. Ottey, C. Kurischko, P. Hieter, and F. C. Luca The Mitotic Exit Network Mob1p-Dbf2p Kinase Complex Localizes to the Nucleus and Regulates Passenger Protein Localization Mol. Biol. Cell, December 1, 2005; 16(12): 5465 - 5479. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Dobson, A. J. Pickett, S. Velmurugan, J. B. Pinder, L. A. Barrett, M. Jayaram, and J. S. K. Chew The 2{micro}m Plasmid Causes Cell Death in Saccharomyces cerevisiae with a Mutation in Ulp1 Protease Mol. Cell. Biol., May 15, 2005; 25(10): 4299 - 4310. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-m. Li, Y. Li, and S. J. Elledge Genetic Analysis of the Kinetochore DASH Complex Reveals an Antagonistic Relationship with the Ras/Protein Kinase A Pathway and a Novel Subunit Required for Ask1 Association Mol. Cell. Biol., January 15, 2005; 25(2): 767 - 778. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Morey, K. Barnes, Y. Chen, M. Fitzgerald-Hayes, and R. E. Baker The Histone Fold Domain of Cse4 Is Sufficient for CEN Targeting and Propagation of Active Centromeres in Budding Yeast Eukaryot. Cell, December 1, 2004; 3(6): 1533 - 1543. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Capiaghi, T. V. Ho, and F. Thoma Kinetochores Prevent Repair of UV Damage in Saccharomyces cerevisiae Centromeres Mol. Cell. Biol., August 15, 2004; 24(16): 6907 - 6918. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Wieland, S. Orthaus, S. Ohndorf, S. Diekmann, and P. Hemmerich Functional Complementation of Human Centromere Protein A (CENP-A) by Cse4p from Saccharomyces cerevisiae Mol. Cell. Biol., August 1, 2004; 24(15): 6620 - 6630. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. A. Kent, S. M. Eibert, and J. Mellor Cbf1p Is Required for Chromatin Remodeling at Promoter-proximal CACGTG Motifs in Yeast J. Biol. Chem., June 25, 2004; 279(26): 27116 - 27123. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. K. Baetz, N. J. Krogan, A. Emili, J. Greenblatt, and P. Hieter The ctf13-30/CTF13 Genomic Haploinsufficiency Modifier Screen Identifies the Yeast Chromatin Remodeling Complex RSC, Which Is Required for the Establishment of Sister Chromatid Cohesion Mol. Cell. Biol., February 1, 2004; 24(3): 1232 - 1244. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Kagansky, L. Freeman, D. Lukyanov, and A. Strunnikov Histone Tail-independent Chromatin Binding Activity of Recombinant Cohesin Holocomplex J. Biol. Chem., January 30, 2004; 279(5): 3382 - 3388. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. S. Nekrasov, M. A. Smith, S. Peak-Chew, and J. V. Kilmartin Interactions between Centromere Complexes in Saccharomyces cerevisiae Mol. Biol. Cell, December 1, 2003; 14(12): 4931 - 4946. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. W. Espelin, K. T. Simons, S. C. Harrison, and P. K. Sorger Binding of the Essential Saccharomyces cerevisiae Kinetochore Protein Ndc10p to CDEII Mol. Biol. Cell, November 1, 2003; 14(11): 4557 - 4568. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Westermann, I. M. Cheeseman, S. Anderson, J. R. Yates III, D. G. Drubin, and G. Barnes Architecture of the budding yeast kinetochore reveals a conserved molecular core J. Cell Biol., October 27, 2003; 163(2): 215 - 222. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Kerscher, L. B. Crotti, and M. A. Basrai Recognizing Chromosomes in Trouble: Association of the Spindle Checkpoint Protein Bub3p with Altered Kinetochores and a Unique Defective Centromere Mol. Cell. Biol., September 15, 2003; 23(18): 6406 - 6418. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-m. Hsu, J. Huang, P. B. Meluh, and B. C. Laurent The Yeast RSC Chromatin-Remodeling Complex Is Required for Kinetochore Function in Chromosome Segregation Mol. Cell. Biol., May 1, 2003; 23(9): 3202 - 3215. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Pot, V. Measday, B. Snydsman, G. Cagney, S. Fields, T. N. Davis, E. G.D. Muller, and P. Hieter Chl4p and Iml3p Are Two New Members of the Budding Yeast Outer Kinetochore Mol. Biol. Cell, February 1, 2003; 14(2): 460 - 476. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. A. Sclafani, M. Tecklenburg, and A. Pierce The mcm5-bob1 Bypass of Cdc7p/Dbf4p in DNA Replication Depends on Both Cdk1-Independent and Cdk1-Dependent Steps in Saccharomyces cerevisiae Genetics, May 1, 2002; 161(1): 47 - 57. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. M. Cheeseman, D. G. Drubin, and G. Barnes Simple centromere, complex kinetochore: linking spindle microtubules and centromeric DNA in budding yeast J. Cell Biol., April 15, 2002; 157(2): 199 - 203. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Li, J. Bachant, A. A. Alcasabas, Y. Wang, J. Qin, and S. J. Elledge The mitotic spindle is required for loading of the DASH complex onto the kinetochore Genes & Dev., January 15, 2002; 16(2): 183 - 197. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Sharp, A. A. Franco, M. A. Osley, and P. D. Kaufman Chromatin assembly factor I and Hir proteins contribute to building functional kinetochores in S. cerevisiae Genes & Dev., January 1, 2002; 16(1): 85 - 100. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Measday, D. W. Hailey, I. Pot, S. A. Givan, K. M. Hyland, G. Cagney, S. Fields, T. N. Davis, and P. Hieter Ctf3p, the Mis6 budding yeast homolog, interacts with Mcm22p and Mcm16p at the yeast outer kinetochore Genes & Dev., January 1, 2002; 16(1): 101 - 113. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Biggins, N. Bhalla, A. Chang, D. L. Smith, and A. W. Murray Genes Involved in Sister Chromatid Separation and Segregation in the Budding Yeast Saccharomyces cerevisiae Genetics, October 1, 2001; 159(2): 453 - 470. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Enquist-Newman, I. M. Cheeseman, D. Van Goor, D. G. Drubin, P. B. Meluh, and G. Barnes Dad1p, Third Component of the Duo1p/Dam1p Complex Involved in Kinetochore Function and Mitotic Spindle Integrity Mol. Biol. Cell, September 1, 2001; 12(9): 2601 - 2613. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. J. Craven and T. D. Petes The Saccharomyces cerevisiae Suppressor of Choline Sensitivity (SCS2) Gene Is a Multicopy Suppressor of mec1 Telomeric Silencing Defects Genetics, May 1, 2001; 158(1): 145 - 154. [Abstract] [Full Text]
|
|
|
|
|
|
K. Ahmad and S. Henikoff Centromeres Are Specialized Replication Domains in Heterochromatin J. Cell Biol., April 2, 2001; 153(1): 101 - 110. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. D. Gardner, A. Poddar, C. Yellman, P. A. Tavormina, M. C. Monteagudo, and D. J. Burke The Spindle Checkpoint of the Yeast Saccharomyces cerevisiae Requires Kinetochore Function and Maps to the CBF3 Domain Genetics, April 1, 2001; 157(4): 1493 - 1502. [Abstract] [Full Text]
|
|
|
|
|
|
O. Kerscher, P. Hieter, M. Winey, and M. A. Basrai Novel Role for a Saccharomyces cerevisiae Nucleoporin, Nup170p, in Chromosome Segregation Genetics, April 1, 2001; 157(4): 1543 - 1553. [Abstract] [Full Text]
|
|
|
|
 |
|
 G. Wieland, P. Hemmerich, M. Koch, T. Stoyan, J. Hegemann, and S. Diekmann Determination of the binding constants of the centromere protein Cbf1 to all 16 centromere DNAs of Saccharomyces cerevisiae Nucleic Acids Res., March 1, 2001; 29(5): 1054 - 1060. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Zaman, A. Z. Ansari, S. S. Koh, R. Young, and M. Ptashne Interaction of a transcriptional repressor with the RNA polymerase II holoenzyme plays a crucial role in repression PNAS, February 27, 2001; 98(5): 2550 - 2554. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. A. Wigge and J. V. Kilmartin The Ndc80p Complex from Saccharomyces cerevisiae Contains Conserved Centromere Components and Has a Function in Chromosome Segregation J. Cell Biol., January 22, 2001; 152(2): 349 - 360. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. A. Van Hooser, I. I. Ouspenski, H. C. Gregson, D. A. Starr, T. J. Yen, M. L. Goldberg, K. Yokomori, W. C. Earnshaw, K. F. Sullivan, and B. R. Brinkley Specification of kinetochore-forming chromatin by the histone H3 variant CENP-A J. Cell Sci., January 10, 2001; 114(19): 3529 - 3542. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. M. Cheeseman, M. Enquist-Newman, T. Muller-Reichert, D. G. Drubin, and G. Barnes Mitotic Spindle Integrity and Kinetochore Function Linked by the Duo1p/Dam1p Complex J. Cell Biol., January 8, 2001; 152(1): 197 - 212. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. D. Urnov and A. P. Wolffe A Necessary Good: Nuclear Hormone Receptors and Their Chromatin Templates Mol. Endocrinol., January 1, 2001; 15(1): 1 - 16. [Full Text]
|
|
|
|
|
|
S. Laloraya, V. Guacci, and D. Koshland Chromosomal Addresses of the Cohesin Component Mcd1p J. Cell Biol., November 20, 2000; 151(5): 1047 - 1056. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Hemmerich, T. Stoyan, G. Wieland, M. Koch, J. Lechner, and S. Diekmann Interaction of yeast kinetochore proteins with centromere-protein/transcription factor Cbf1 PNAS, November 7, 2000; 97(23): 12583 - 12588. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. V. Skibbens Holding Your Own: Establishing Sister Chromatid Cohesion Genome Res., November 1, 2000; 10(11): 1664 - 1671. [Full Text]
|
|
|
|
|
|
Y. Chen, R. E. Baker, K. C. Keith, K. Harris, S. Stoler, and M. Fitzgerald-Hayes The N Terminus of the Centromere H3-Like Protein Cse4p Performs an Essential Function Distinct from That of the Histone Fold Domain Mol. Cell. Biol., September 15, 2000; 20(18): 7037 - 7048. [Abstract] [Full Text]
|
|
|
|
|
|
L. Glowczewski, P. Yang, T. Kalashnikova, M. S. Santisteban, and M. M. Smith Histone-Histone Interactions and Centromere Function Mol. Cell. Biol., August 1, 2000; 20(15): 5700 - 5711. [Abstract] [Full Text]
|
|
|
|
|
|
L. Freeman, L. Aragon-Alcaide, and A. Strunnikov The Condensin Complex Governs Chromosome Condensation and Mitotic Transmission of rDNA J. Cell Biol., May 15, 2000; 149(4): 811 - 824. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Zheng, Y. Chen, D. J. Riley, P.-L. Chen, and W.-H. Lee Retinoblastoma Protein Enhances the Fidelity of Chromosome Segregation Mediated by hsHec1p Mol. Cell. Biol., May 15, 2000; 20(10): 3529 - 3537. [Abstract] [Full Text]
|
|
|
|
|
|
H. Lin, J. H. Choi, J. Hasek, N. DeLillo, W. Lou, and A. Vancura Phospholipase C Is Involved in Kinetochore Function in Saccharomyces cerevisiae Mol. Cell. Biol., May 15, 2000; 20(10): 3597 - 3607. [Abstract] [Full Text]
|
|
|
|
|
|
J. F. Partridge, B. Borgstrøm, and R. C. Allshire Distinct protein interaction domains and protein spreading in a complex centromere Genes & Dev., April 1, 2000; 14(7): 783 - 791. [Abstract] [Full Text]
|
|
|
|
|
|
L. D. Nelson, M. Musso, and M. W. Van Dyke The Yeast STM1 Gene Encodes a Purine Motif Triple Helical DNA-binding Protein J. Biol. Chem., February 25, 2000; 275(8): 5573 - 5581. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Earle, A. Saxena, A. MacDonald, D. F. Hudson, L. G. Shaffer, R. Saffery, M. R. Cancilla, S. M. Cutts, E. Howman, and K. H. A. Choo Poly(ADP-ribose) polymerase at active centromeres and neocentromeres at metaphase Hum. Mol. Genet., January 22, 2000; 9(2): 187 - 194. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Toth and M. D. Biggin The specificity of protein-DNA crosslinking by formaldehyde: in vitro and in Drosophila embryos Nucleic Acids Res., January 15, 2000; 28(2): e4 - e4. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Zheng, Y. Chen, and W.-H. Lee Hec1p, an Evolutionarily Conserved Coiled-Coil Protein, Modulates Chromosome Segregation through Interaction with SMC Proteins Mol. Cell. Biol., August 1, 1999; 19(8): 5417 - 5428. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Zeng, J. A. Kahana, P. A. Silver, M. K. Morphew, J. R. McIntosh, I. T. Fitch, J. Carbon, and W. S. Saunders Slk19p Is a Centromere Protein That Functions to Stabilize Mitotic Spindles J. Cell Biol., July 26, 1999; 146(2): 415 - 426. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. C. Megee and D. Koshland A Functional Assay for Centromere-Associated Sister Chromatid Cohesion Science, July 9, 1999; 285(5425): 254 - 257. [Abstract] [Full Text]
|
|
|
|
 |
|
 R. K. Dawe, L. M. Reed, H.-G. Yu, M. G. Muszynski, and E. N. Hiatt A Maize Homolog of Mammalian CENPC Is a Constitutive Component of the Inner Kinetochore PLANT CELL, July 1, 1999; 11(7): 1227 - 1238. [Abstract] [Full Text]
|
|
|
|
|
|
M. H. Jones, J. B. Bachant, A. R. Castillo, T. H. Giddings Jr., and M. Winey Yeast Dam1p Is Required to Maintain Spindle Integrity during Mitosis and Interacts with the Mps1p Kinase Mol. Biol. Cell, July 1, 1999; 10(7): 2377 - 2391. [Abstract] [Full Text]
|
|
|
|
|
|
H.-G. Yu, M. G. Muszynski, and R. Kelly Dawe The Maize Homologue of the Cell Cycle Checkpoint Protein MAD2 Reveals Kinetochore Substructure and Contrasting Mitotic and Meiotic Localization Patterns J. Cell Biol., May 3, 1999; 145(3): 425 - 435. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Ortiz, O. Stemmann, S. Rank, and J. Lechner A putative protein complex consisting of Ctf19, Mcm21, and Okp1 represents a missing link in the budding yeast kinetochore Genes & Dev., May 1, 1999; 13(9): 1140 - 1155. [Abstract] [Full Text]
|
|
|
|
|
|
K. M. Hyland, J. Kingsbury, D. Koshland, and P. Hieter Ctf19p: A Novel Kinetochore Protein in Saccharomyces cerevisiae and a Potential Link between the Kinetochore and Mitotic Spindle J. Cell Biol., April 5, 1999; 145(1): 15 - 28. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
|
