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Vol. 12, No. 10, pp. 1483-1494, May 15, 1998
Cancer Research Campaign (CRC) Laboratories, Cell Cycle Genetics
Research Group, Department of Anatomy and Physiology, Medical Sciences
Institute, University of Dundee, Dundee DD1 4HN, UK;
1 Howard
Hughes Medical Institute, Baylor College of Medicine, Department of
Molecular and Human Genetics, Houston, Texas 77030 USA
Mutations in the Drosophila gene pavarotti result
in the formation of abnormally large cells in the embryonic nervous
system. In mitotic cycle 16, cells of pav mutant embryos
undergo normal anaphase but then develop an abnormal telophase spindle
and fail to undertake cytokinesis. We show that the septin Peanut,
actin, and the actin-associated protein Anillin, do not become
correctly localized in pav mutants. pav encodes a
kinesin-like protein, PAV-KLP, related to the mammalian MKLP-1. In
cellularized embryos, the protein is localized to centrosomes early in
mitosis, and to the midbody region of the spindle in late anaphase and
telophase. We show that Polo kinase associates with PAV-KLP with which
it shows an overlapping pattern of subcellular localization during the
mitotic cycle and this distribution is disrupted in pav
mutants. We suggest that PAV-KLP is required both to establish the
structure of the telophase spindle to provide a framework for the
assembly of the contractile ring, and to mobilize mitotic regulator proteins.
[Key Words: Cytokinesis; kinesin-like protein; Polo kinase; Drosophila]
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