.GIF?ad=16177&adview=true)
![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 12, No. 11, pp. 1678-1690, June 1, 1998
1 Program in Cellular and Molecular Biology,
2 Department of Biological Chemistry, University of Michigan
Medical School, Ann Arbor, Michigan 48109-0606 USA;
3 Harvard
Microchemistry Facility, Cambridge, Massachusetts 02138 USA
Ribonuclease P (RNase P) is a ribonucleoprotein enzyme that
cleaves precursor tRNA transcripts to give mature 5' ends. RNase P
in eubacteria has a large, catalytic RNA subunit and a small protein
subunit that are required for precursor tRNA cleavage in vivo. Although
the eukaryotic holoenzymes have similar, large RNA subunits, previous
work in a number of systems has suggested that the eukaryotic enzymes
require a greater protein content. We have purified the
Saccharomyces cerevisiae nuclear RNase P to apparent
homogeneity, allowing the first comprehensive analysis of an
unexpectedly complex subunit composition. Peptide sequencing by ion
trap mass spectrometry identifies nine proteins that copurify with the
nuclear RNase P RNA subunit, totaling 20-fold more protein than in the
bacterial enzyme. All of these proteins are encoded by genes essential
for RNase P activity and for cell viability. Previous genetic studies
suggested that four proteins might be subunits of both RNase P and
RNase MRP, the related rRNA processing enzyme. We demonstrate that all
four of these proteins, Pop1p, Pop3p, Pop4p, and Rpp1p, are integral
subunits of RNase P. In addition, four of the five newly identified
protein subunits, Pop5p, Pop6p, Pop7p, and Pop8p, also appear to be
shared between RNase P and RNase MRP. Only one polypeptide, Rpr2p, is
unique to the RNase P holoenzyme by genetic depletion and
immunoprecipitation studies. The large increase in the number of
protein subunits over eubacterial RNase P is consistent with an
increase in functional complexity in eukaryotes. The degree of
structural similarity between nuclear RNase P and RNase MRP suggests
that some aspects of their functions in pre-tRNA and pre-rRNA
processing pathways might overlap or be coordinated.
[Key Words: Ribonuclease P; ribonuclease MRP; RNP; tRNA]
CiteULike 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  T. V. Aspinall, J. M.B. Gordon, H. J. Bennett, P. Karahalios, J.-P. Bukowski, S. C. Walker, D. R. Engelke, and J. M. Avis Interactions between subunits of Saccharomyces cerevisiae RNase MRP support a conserved eukaryotic RNase P/MRP architecture Nucleic Acids Res., October 8, 2007; 35(19): 6439 - 6450. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Perederina, O. Esakova, H. Koc, M. E. Schmitt, and A. S. Krasilnikov Specific binding of a Pop6/Pop7 heterodimer to the P3 stem of the yeast RNase MRP and RNase P RNAs RNA, October 1, 2007; 13(10): 1648 - 1655. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Chen, T. S. Rozhdestvensky, L. J. Collins, J. Schmitz, and D. Penny Combined experimental and computational approach to identify non-protein-coding RNAs in the deep-branching eukaryote Giardia intestinalis Nucleic Acids Res., July 9, 2007; 35(14): 4619 - 4628. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H.-Y. Tsai, D. K. Pulukkunat, W. K. Woznick, and V. Gopalan Functional reconstitution and characterization of Pyrococcus furiosus RNase P PNAS, October 31, 2006; 103(44): 16147 - 16152. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Rosenblad, M. D. Lopez, P. Piccinelli, and T. Samuelsson Inventory and analysis of the protein subunits of the ribonucleases P and MRP provides further evidence of homology between the yeast and human enzymes Nucleic Acids Res., October 6, 2006; 34(18): 5145 - 5156. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. J.M. Welting, B. J. Kikkert, W. J. van Venrooij, and G. J.M. Pruijn Differential association of protein subunits with the human RNase MRP and RNase P complexes RNA, July 1, 2006; 12(7): 1373 - 1382. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Xiao, J. Hsieh, R. L. Nugent, D. J. Coughlin, C. A. Fierke, and D. R. Engelke Functional characterization of the conserved amino acids in Pop1p, the largest common protein subunit of yeast RNases P and MRP RNA, June 1, 2006; 12(6): 1023 - 1037. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Zhu, V. Stribinskis, K. S. Ramos, and Y. Li Sequence analysis of RNase MRP RNA reveals its origination from eukaryotic RNase P RNA RNA, May 1, 2006; 12(5): 699 - 706. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Gill, J. Aulds, and M. E. Schmitt A specialized processing body that is temporally and asymmetrically regulated during the cell cycle in Saccharomyces cerevisiae J. Cell Biol., April 10, 2006; 173(1): 35 - 45. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Schimanski, T. N. Nguyen, and A. Gunzl Highly Efficient Tandem Affinity Purification of Trypanosome Protein Complexes Based on a Novel Epitope Combination Eukaryot. Cell, November 1, 2005; 4(11): 1942 - 1950. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. XIAO, J. J. DAY-STORMS, C. SRISAWAT, C. A. FIERKE, and D. R. ENGELKE Characterization of conserved sequence elements in eukaryotic RNase P RNA reveals roles in holoenzyme assembly and tRNA processing RNA, June 1, 2005; 11(6): 885 - 896. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. MARQUEZ, J. K. HARRIS, S. T. KELLEY, J. W. BROWN, S. C. DAWSON, E. C. ROBERTS, and N. R. PACE Structural implications of novel diversity in eucaryal RNase P RNA RNA, May 1, 2005; 11(5): 739 - 751. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Salinas, S. Wierzbicki, L. Zhou, and M. E. Schmitt Characterization and Purification of Saccharomyces cerevisiae RNase MRP Reveals a New Unique Protein Component J. Biol. Chem., March 25, 2005; 280(12): 11352 - 11360. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. DLAKIC 3D models of yeast RNase P/MRP proteins Rpp1p and Pop3p RNA, February 1, 2005; 11(2): 123 - 127. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. J. DAY-STORMS, S. NIRANJANAKUMARI, and C. A. FIERKE Ionic interactions between PRNA and P protein in Bacillus subtilis RNase P characterized using a magnetocapture-based assay RNA, October 20, 2004; 10(10): 1595 - 1608. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Pertschy, G. Zisser, H. Schein, R. Koffel, G. Rauch, K. Grillitsch, C. Morgenstern, M. Durchschlag, G. Hogenauer, and H. Bergler Diazaborine Treatment of Yeast Cells Inhibits Maturation of the 60S Ribosomal Subunit Mol. Cell. Biol., July 15, 2004; 24(14): 6476 - 6487. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Li, S. Zaman, Y. Langdon, J. M. Zengel, and L. Lindahl Identification of a functional core in the RNA component of RNase MRP of budding yeasts Nucleic Acids Res., July 14, 2004; 32(12): 3703 - 3711. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Aloy, B. Bottcher, H. Ceulemans, C. Leutwein, C. Mellwig, S. Fischer, A.-C. Gavin, P. Bork, G. Superti-Furga, L. Serrano, et al. Structure-Based Assembly of Protein Complexes in Yeast Science, March 26, 2004; 303(5666): 2026 - 2029. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Gill, T. Cai, J. Aulds, S. Wierzbicki, and M. E. Schmitt RNase MRP Cleaves the CLB2 mRNA To Promote Cell Cycle Progression: Novel Method of mRNA Degradation Mol. Cell. Biol., February 1, 2004; 24(3): 945 - 953. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. P. Boomershine, C. A. McElroy, H.-Y. Tsai, R. C. Wilson, V. Gopalan, and M. P. Foster Structure of Mth11/Mth Rpp29, an essential protein subunit of archaeal and eukaryotic RNase P PNAS, December 23, 2003; 100(26): 15398 - 15403. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Cohen, R. Reiner, and N. Jarrous Alterations in the intracellular level of a protein subunit of human RNase P affect processing of tRNA precursors Nucleic Acids Res., August 15, 2003; 31(16): 4836 - 4846. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Yoshihisa, K. Yunoki-Esaki, C. Ohshima, N. Tanaka, and T. Endo Possibility of Cytoplasmic pre-tRNA Splicing: the Yeast tRNA Splicing Endonuclease Mainly Localizes on the Mitochondria Mol. Biol. Cell, August 1, 2003; 14(8): 3266 - 3279. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. K. Hopper and E. M. Phizicky tRNA transfers to the limelight Genes & Dev., January 15, 2003; 17(2): 162 - 180. [Full Text] [PDF]
|
|
|
|
|
|
Y. Li and S. Altman Partial reconstitution of human RNase P in HeLa cells between its RNA subunit with an affinity tag and the intact protein components Nucleic Acids Res., September 1, 2002; 30(17): 3706 - 3711. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Cai, J. Aulds, T. Gill, M. Cerio, and M. E. Schmitt The Saccharomyces cerevisiae RNase Mitochondrial RNA Processing Is Critical for Cell Cycle Progression at the End of Mitosis Genetics, July 1, 2002; 161(3): 1029 - 1042. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. D. Abeyrathne, A. I. Lalev, and R. N. Nazar A RAC Protein-binding Site in the Internal Transcribed Spacer 2 of Pre-rRNA Transcripts from Schizosaccharomyces pombe J. Biol. Chem., June 7, 2002; 277(24): 21291 - 21299. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. H. P. Tan, R. Pach, A. Crausaz, A. Ivens, and A. Schneider tRNAs in Trypanosoma brucei: Genomic Organization, Expression, and Mitochondrial Import Mol. Cell. Biol., June 1, 2002; 22(11): 3707 - 3717. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Ishiguro, G. A. Kassavetis, and E. P. Geiduschek Essential Roles of Bdp1, a Subunit of RNA Polymerase III Initiation Factor TFIIIB, in Transcription and tRNA Processing Mol. Cell. Biol., May 15, 2002; 22(10): 3264 - 3275. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Houser-Scott, S. Xiao, C. E. Millikin, J. M. Zengel, L. Lindahl, and D. R. Engelke Interactions among the protein and RNA subunits of Saccharomyces cerevisiae nuclear RNase P PNAS, March 5, 2002; 99(5): 2684 - 2689. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. M. Brusca, H. L. True, and D. W. Celander Novel RNA-binding Properties of Pop3p Support a Role for Eukaryotic RNase P Protein Subunits in Substrate Recognition J. Biol. Chem., November 2, 2001; 276(45): 42543 - 42548. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. van Eenennaam, A. van der Heijden, R. J. R. J. Janssen, W. J. van Venrooij, and G. J. M. Pruijn Basic Domains Target Protein Subunits of the RNase MRP Complex to the Nucleolus Independently of Complex Association Mol. Biol. Cell, November 1, 2001; 12(11): 3680 - 3689. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Pogacic, F. Dragon, and W. Filipowicz Human H/ACA Small Nucleolar RNPs and Telomerase Share Evolutionarily Conserved Proteins NHP2 and NOP10 Mol. Cell. Biol., December 1, 2000; 20(23): 9028 - 9040. [Abstract] [Full Text]
|
|
|
|
|
|
S. Fath, P. Milkereit, A. V. Podtelejnikov, N. Bischler, P. Schultz, M. Bier, M. Mann, and H. Tschochner Association of Yeast RNA Polymerase I with a Nucleolar Substructure Active in rRNA Synthesis and Processing J. Cell Biol., May 1, 2000; 149(3): 575 - 590. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Dragon and W. Filipowicz In Vitro Assembly of Human H/ACA Small Nucleolar RNPs Reveals Unique Features of U17 and Telomerase RNAs Mol. Cell. Biol., May 1, 2000; 20(9): 3037 - 3048. [Abstract] [Full Text]
|
|
|
|
|
|
A. D. Omer, T. M. Lowe, A. G. Russell, H. Ebhardt, S. R. Eddy, and P. P. Dennis Homologs of Small Nucleolar RNAs in Archaea Science, April 21, 2000; 288(5465): 517 - 522. [Abstract] [Full Text]
|
|
|
|
|
|
D. L. J. Lafontaine and D. Tollervey Synthesis and Assembly of the Box C+D Small Nucleolar RNPs Mol. Cell. Biol., April 15, 2000; 20(8): 2650 - 2659. [Abstract] [Full Text]
|
|
|
|
|
|
H. Qiu, C. Hu, J. Anderson, G. R. Björk, S. Sarkar, A. K. Hopper, and A. G. Hinnebusch Defects in tRNA Processing and Nuclear Export Induce GCN4 Translation Independently of Phosphorylation of the alpha Subunit of Eukaryotic Translation Initiation Factor 2 Mol. Cell. Biol., April 1, 2000; 20(7): 2505 - 2516. [Abstract] [Full Text]
|
|
|
|
|
|
H. Grosshans, E. Hurt, and G. Simos An aminoacylation-dependent nuclear tRNA export pathway in yeast Genes & Dev., April 1, 2000; 14(7): 830 - 840. [Abstract] [Full Text]
|
|
|
|
|
|
D. Kressler, P. Linder, and J. de la Cruz Protein trans-Acting Factors Involved in Ribosome Biogenesis in Saccharomyces cerevisiae Mol. Cell. Biol., December 1, 1999; 19(12): 7897 - 7912. [Full Text] [PDF]
|
|
|
|
|
|
T. Cai, T. R. Reilly, M. Cerio, and M. E. Schmitt Mutagenesis of SNM1, Which Encodes a Protein Component of the Yeast RNase MRP, Reveals a Role for This Ribonucleoprotein Endoribonuclease in Plasmid Segregation Mol. Cell. Biol., November 1, 1999; 19(11): 7857 - 7869. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Jarrous, J. S. Wolenski, D. Wesolowski, C. Lee, and S. Altman Localization in the Nucleolus and Coiled Bodies of Protein Subunits of the Ribonucleoprotein Ribonuclease P J. Cell Biol., August 9, 1999; 146(3): 559 - 572. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Pannucci, E. S. Haas, T. A. Hall, J. K. Harris, and J. W. Brown RNase P RNAs from some Archaea are catalytically active PNAS, July 6, 1999; 96(14): 7803 - 7808. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Calvo, R. Cuesta, J. Anderson, N. Gutierrez, M. T. Garcia-Barrio, A. G. Hinnebusch, and M. Tamame GCD14p, a Repressor of GCN4 Translation, Cooperates with Gcd10p and Lhp1p in the Maturation of Initiator Methionyl-tRNA in Saccharomyces cerevisiae Mol. Cell. Biol., June 1, 1999; 19(6): 4167 - 4181. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. L. Wolin and A. G. Matera The trials and travels of tRNA Genes & Dev., January 1, 1999; 13(1): 1 - 10. [Full Text]
|
|
|
|
|
|
H. van Eenennaam, D. Lugtenberg, J. H. P. Vogelzangs, W. J. van Venrooij, and G. J. M. Pruijn hPop5, a Protein Subunit of the Human RNase MRP and RNase P Endoribonucleases J. Biol. Chem., August 17, 2001; 276(34): 31635 - 31641. [Abstract] [Full Text] [PDF]
|
|
