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Vol. 12, No. 16, pp. 2463-2468, August 15, 1998
1 Institut de Genetique Moleculaire de
Montpellier-Centre National de la Recherche Scientifique (CNRS), 34033 Montpellier Cedex 01, France;
2 Department of Biological
Chemistry and Program in Cellular and Molecular Biology, The University
of Michigan, Ann Arbor, Michigan 48109-0606 USA;
3 Department
of Anatomy and Structural Biology and Cell Biology, Albert Einstein
College of Medicine, New York, New York 10461 USA
There is little information as to the location of early tRNA
biosynthesis. Using fluorescent in situ hybridization in the budding
yeast, Saccharomyces cerevisiae, examples of nuclear pre-tRNAs are shown to reside primarily in the nucleoli. We also probed the RNA
subunit of RNase P. The majority of the signal from RNase P probes was
nucleolar, with less intense signals in the nucleoplasm. These results
demonstrate that a major portion of the tRNA processing pathway is
compartmentalized in nucleoli with rRNA synthesis and ribosomal
assembly. The spatial juxtaposition suggests the possibility of direct
coordination between tRNA and ribosome biosynthesis.
[Key Words: RNase P; nucleolus; tRNA processing]
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