![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 12, No. 18, pp. 2921-2931, September 15, 1998
Department of Biochemistry and Biophysics, Texas A&M University,
College Station, Texas 77843-2128 USA
In Euplotes crassus, telomerase is responsible for telomere
maintenance during vegetative growth and de novo telomere synthesis during macronuclear development. Here we show that telomerase in the
vegetative stage of the life cycle exists as a 280-kD complex that can
add telomeric repeats only onto telomeric DNA primers. Following the
initiation of macronuclear development, telomerase assembles into
larger complexes of 550 kD, 1600 kD, and 5 MD. In the 1600-kDa and
5-MDa complexes, telomerase is more processive than in the two smaller
complexes and can add telomeres de novo onto nontelomeric 3' ends.
Assembly of higher order telomerase complexes is accompanied by an
extended region of RNase V1 and RNase T1 protection in the telomerase
RNA subunit that is not observed with telomerase from vegetatively
growing cells. The protected residues encompass a highly conserved
region previously proposed to serve as a platform for formation of
higher order structures. These findings provide the first direct
demonstration of developmentally regulated higher order telomerase
complexes with unique biochemical and structural properties.
[Key Words: Telomerase; chromosome healing; Euplotes crassus; RNA footprinting; holoenzyme]
CiteULike 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  S. W. Yang, S. K. Kim, and W. T. Kim Perturbation of NgTRF1 Expression Induces Apoptosis-Like Cell Death in Tobacco BY-2 Cells and Implicates NgTRF1 in the Control of Telomere Length and Stability PLANT CELL, December 1, 2004; 16(12): 3370 - 3385. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. P. Munoz and K. Collins Biochemical properties of Trypanosoma cruzi telomerase Nucleic Acids Res., September 30, 2004; 32(17): 5214 - 5222. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. AIGNER and T. R. CECH The Euplotes telomerase subunit p43 stimulates enzymatic activity and processivity in vitro RNA, July 1, 2004; 10(7): 1108 - 1118. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. L. Witkin and K. Collins Holoenzyme proteins required for the physiological assembly and activity of telomerase Genes & Dev., May 15, 2004; 18(10): 1107 - 1118. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Lin and E. H. Blackburn Nucleolar protein PinX1p regulates telomerase by sequestering its protein catalytic subunit in an inactive complex lacking telomerase RNA Genes & Dev., February 15, 2004; 18(4): 387 - 396. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. F. Lue, Y.-C. Lin, and I. S. Mian A Conserved Telomerase Motif within the Catalytic Domain of Telomerase Reverse Transcriptase Is Specifically Required for Repeat Addition Processivity Mol. Cell. Biol., December 1, 2003; 23(23): 8440 - 8449. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Huard, T. J. Moriarty, and C. Autexier The C terminus of the human telomerase reverse transcriptase is a determinant of enzyme processivity Nucleic Acids Res., July 15, 2003; 31(14): 4059 - 4070. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Leeper, N. Leulliot, and G. Varani The solution structure of an essential stem-loop of human telomerase RNA Nucleic Acids Res., May 15, 2003; 31(10): 2614 - 2621. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Wang, S. R. Dean, and D. E. Shippen Oligomerization of the telomerase reverse transcriptase from Euplotes crassus Nucleic Acids Res., September 15, 2002; 30(18): 4032 - 4039. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Ray, Z. Karamysheva, L. Wang, D. E. Shippen, and C. M. Price Interactions between Telomerase and Primase Physically Link the Telomere and Chromosome Replication Machinery Mol. Cell. Biol., August 15, 2002; 22(16): 5859 - 5868. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. X. Mason, C. Autexier, and C. W. Greider Tetrahymena proteins p80 and p95 are not core telomerase components PNAS, October 5, 2001; (2001) 221456398. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Akalin, L. W. Elmore, H. L. Forsythe, B. A. Amaker, E. D. McCollum, P. S. Nelson, J. L. Ware, and S. E. Holt A Novel Mechanism for Chaperone-mediated Telomerase Regulation during Prostate Cancer Progression Cancer Res., June 1, 2001; 61(12): 4791 - 4796. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-P. LIU Studies of the molecular mechanisms in the regulation of telomerase activity FASEB J, December 1, 1999; 13(15): 2091 - 2104. [Abstract] [Full Text]
|
|
|
|
|
|
J. D. Licht and K. Collins Telomerase RNA function in recombinant Tetrahymena telomerase Genes & Dev., May 1, 1999; 13(9): 1116 - 1125. [Abstract] [Full Text]
|
|
|
|
 |
|
 T. M. Bryan, K. J. Goodrich, and T. R. Cech A Mutant of Tetrahymena Telomerase Reverse Transcriptase with Increased Processivity J. Biol. Chem., July 28, 2000; 275(31): 24199 - 24207. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. D. Hardy, C. S. Schultz, and K. Collins Requirements for the dGTP-dependent Repeat Addition Processivity of Recombinant Tetrahymena Telomerase J. Biol. Chem., February 9, 2001; 276(7): 4863 - 4871. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. X. Mason, C. Autexier, and C. W. Greider Tetrahymena proteins p80 and p95 are not core telomerase components PNAS, October 23, 2001; 98(22): 12368 - 12373. [Abstract] [Full Text] [PDF]
|
|
