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Vol. 12, No. 22, pp. 3603-3612, November 15, 1998
1 Institute of Neuroscience, University of Oregon, Eugene,
Oregon 97403 USA;
2 Department of Cell and Structural Biology,
University of Illinois/Howard Hughes Medical Institute
(HHMI), Urbana, Illinois 61801;
3 Department of Developmental
Biology, HHMI, Stanford University School of Medicine, Stanford,
California 94305 USA;
4 Pediatric Neurology Research
Laboratories, University of Michigan Medical Center,
Ann Arbor, Michigan 48109 USA
The Drosophila CNS develops from three columns of
neuroectodermal cells along the dorsoventral (DV) axis: ventral,
intermediate, and dorsal. In this and the accompanying paper, we
investigate the role of two homeobox genes, vnd and
ind, in establishing ventral and intermediate cell fates within
the Drosophila CNS. During early neurogenesis, Vnd protein is
restricted to ventral column neuroectoderm and neuroblasts; later it is
detected in a complex pattern of neurons. We use molecular markers that
distinguish ventral, intermediate, and dorsal column neuroectoderm and
neuroblasts, and a cell lineage marker for selected neuroblasts, to
show that loss of vnd transforms ventral into intermediate
column identity and that specific ventral neuroblasts fail to form.
Conversely, ectopic vnd produces an intermediate to ventral
column transformation. Thus, vnd is necessary and sufficient to
induce ventral fates and repress intermediate fates within the
Drosophila CNS. Vertebrate homologs of vnd
(Nkx2.1 and 2.2) are similarly expressed in the ventral
CNS, raising the possibility that DV patterning within the CNS is
evolutionarily conserved.
[Key Words: NK-2; ind; msh; neuroectoderm; neuroblast; neurogenesis; cell shape]
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