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Vol. 12, No. 5, pp. 745-754, March 1, 1998
subunit of Escherichia coli RNA
polymerase into a transcriptional activator or an
activation target
Department of Microbiology and Molecular Genetics, Harvard Medical
School, Boston, Massachusetts 02115 USA
Evidence obtained in both eukaryotes and prokaryotes indicates
that arbitrary contacts between DNA-bound proteins and components of
the transcriptional machinery can activate transcription. Here we
demonstrate that the Escherichia coli 
protein, which
copurifies with RNA polymerase, can function as a transcriptional
activator when linked covalently to a DNA-binding protein. We show
further that can function as an activation target when this
covalent linkage is replaced by a pair of interacting polypeptides
fused to the DNA-binding protein and to , respectively. Our findings imply that the protein is associated with RNA polymerase holoenzyme in vivo, and provide support for the hypothesis that contact between a
DNA-bound protein and any component of E. coli RNA polymerase can activate transcription.
[Key Words:
subunit; E. coli; RNA
polymerase; transcriptional activator]
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