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Vol. 12, No. 8, pp. 1134-1144, April 15, 1998
Division of Biological Sciences, Sections of Microbiology and of
Molecular and Cellular Biology, Graduate Group in Microbiology,
University of California, Davis, California 95616 USA
RecQ helicase is important to homologous recombination and DNA
repair in Escherichia coli. We demonstrate that RecQ helicase, in conjunction with RecA and SSB proteins, can initiate recombination events in vitro. In addition, RecQ protein is capable of unwinding a
wide variety of DNA substrates, including joint molecules formed by
RecA protein. These data are consistent with RecQ helicase assuming two
roles in the cell; it can be (1) an initiator of homologous
recombination, or (2) a disrupter of joint molecules formed by aberrant
recombination. These findings also shed light on the function of the
eukaryotic homologs of RecQ helicase, the Sgs1, Blm, and Wrn helicases.
[Key Words: Recombination; DNA repair; RecQ; RecA; helicase]
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|
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|
|
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|
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|
|
|
|
|
|
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|
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|
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|
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|
|
|
|
|
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|
|
|
|
|
|
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|
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