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Vol. 13, No. 10, pp. 1234-1239, May 15, 1999
Department of Biological Sciences, Columbia University,
New York, New York 10027 USA
RNA polymerase II (RNAP II) is responsible for transcription of
mRNA precursors in eukaryotic cells. Recent studies, however, have
suggested that RNAP II also participates in subsequent RNA processing
reactions through interactions between the carboxy-terminal domain
(CTD) of the RNAP II largest subunit and processing factors. Using
reconstituted in vitro splicing assays, we show that RNAP II functions
directly in pre-mRNA splicing by influencing very early steps in
assembly of the spliceosome. We demonstrate that the phosphorylation
status of the CTD dramatically affects activity: Hyperphosphorylated
RNAP IIO strongly activates splicing, whereas hypophosphorylated RNAP
IIA can inhibit the reaction.
[Key Words: RNAP II isoforms; RNA processing; in vitro slicing; phosphorylation]
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