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Vol. 13, No. 12, pp. 1519-1523, June 15, 1999
1 Department of Zoology, Graduate School of Science,
Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan ;
2 Division of Genetics, Department of Molecular and
Cellular Biology, University of California at Berkeley, Berkeley,
California 94720 USA; 3 Department of Biology, University of
California at San Diego, La Jolla, California 92093-0347 USA
The ascidian tadpole represents the most simplified chordate body
plan. It contains a notochord composed of just 40 cells, but as in
vertebrates Brachyury is essential for notochord
differentiation. Here, we show that the misexpression of the
Brachyury gene (Ci-Bra) of Ciona intestinalis
is sufficient to transform endoderm into notochord. Subtractive
hybridization screens were conducted to identify potential
Brachyury target genes that are induced upon Ci-Bra
misexpression. Of 501 independent cDNA clones that were surveyed, 38 were specifically expressed in notochord cells. These potential
Ci-Bra downstream genes appear to encode a broad spectrum of divergent proteins associated with notochord formation.
[Key Words: Ascidians; notochord; Brachyury; downstream genes; differentiation]
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