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Vol. 13, No. 12, pp. 1524-1528, June 15, 1999
1 Hematology Division, Department of Internal Medicine,
University of Utah, Salt Lake City, Utah 84112 USA;
2 Department of Pathology, Emory University, Atlanta, Georgia
30322 USA; 3 Howard Hughes Medical Institute, Department of
Human Genetics, University of Utah School of Medicine,
Salt Lake City, Utah 84112 USA
A procedure is described that directs the self-induced deletion of
DNA sequences as they pass through the male germ line of mice. The
testes-specific promoter from the angiotensin-converting enzyme gene
was used to drive expression of the Cre-recombinase gene. Cre
was linked to the selectable marker Neor, and the two
genes flanked with loxP elements. This cassette was targeted to
the Hoxa3 gene in mouse ES cells that were in turn used to
generate chimeric mice. In these chimeras, somatic cells derived from
the ES cells retained the cassette, but self-excision occurred in all
ES-cell-derived sperm.
[Key Words: Targeted gene modification; conditional mutagenesis; gene therapy; Cre/loxP; angiotensin-converting enzyme]
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