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Vol. 13, No. 14, pp. 1768-1773, July 15, 1999
-catenin from the Axin complex
Howard Hughes Medical Institute (HHMI) and Department of Developmental
Biology, Stanford University School of Medicine, Stanford, California 94305 USA
The stabilization of 
-catenin is a key regulatory step during
cell fate changes and transformations to tumor cells. Several interacting proteins, including Axin, APC, and the protein kinase GSK-3 are implicated in regulating -catenin phosphorylation and
its subsequent degradation. Wnt signaling stabilizes -catenin, but
it was not clear whether and how Wnt signaling regulates the -catenin complex. Here we show that Axin is dephosphorylated in
response to Wnt signaling. The dephosphorylated Axin binds -catenin
less efficiently than the phosphorylated form. Thus, Wnt signaling
lowers Axin's affinity for -catenin, thereby disengaging -catenin from the degradation machinery.
[Key Words:
Axin complex; -catenin; phosphorylation; Wnt signaling]
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|
|
|
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|
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|
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|
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|
|
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|
|
|
|
|
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|
|
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|
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|
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|
|
|
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|
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|
|
|
|
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|
|
|
|
 |
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
 |
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
 |
|
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|
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|
|
|
 |
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|
|
|
|
|
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|
|
|
|
|
|
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