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Vol. 13, No. 14, pp. 1774-1779, July 15, 1999
1 Center for Advanced Biotechnology and Medicine,
2 Graduate Program in Molecular Genetics and Microbiology,
Graduate School of Biomedical Sciences, University of Medicine and
Dentistry of New Jersey, Piscataway, New Jersey 08854 USA
RNA polymerase II nascent transcripts are capped during pausing
before elongation. Here we report that hSPT5, the human homolog of
yeast elongation factor SPT5, interacts directly with the capping enzyme. hSPT5 stimulated capping enzyme guanylylation and mRNA capping
by severalfold. Although RNA 5'-triphosphatase activity was
unaffected, binding to this domain in the full-length enzyme is likely
involved in the stimulation, as hSPT5 did not increase the activity of
the guanylyltransferase fragment. Consistent with capping enzyme
binding, TFIIH-phosphorylated CTD stimulated guanylylation, and this
increase was not additive with hSPT5.
[Key Words: Capping enzyme; hSPT5; P-CTD; transcription elongation]
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