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Vol. 13, No. 15, pp. 1912-1917, August 1, 1999
1 Department of Molecular Genetics, Ohio State
University, Columbus, Ohio 43210-1292 USA; 2 Department of
Biochemistry and Biophysics, University of California- San
Francisco, San Francisco, California 94143-0448 USA
Yeast cells of different cell type exhibit distinct budding
patterns that reflect the organization of the actin cytoskeleton. Bud1p
(Rsr1p), a Ras-like GTPase, and Bud2p, a GTPase-activating protein for
Bud1p, are essential for proper budding pattern. We show that Bud2p is
localized at the presumptive bud site in G1 cells in all cell
types and that this localization is independent of Bud1p. Bud2p
subsequently localizes to the mother-bud neck after bud emergence; this
localization depends on the integrity of the septins. These
observations indicate that Bud2p becomes positioned in G1
cells by recognizing cell type-specific landmarks at the presumptive bud site.
[Key Words: GTPase-activating protein; cell polarity; cell type; yeast]
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