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Vol. 13, No. 15, pp. 1960-1969, August 1, 1999
Howard Hughes Medical Institute, Department of Biochemistry and Cell
Biology MS-140, Rice University, Houston, Texas 77005-1892 USA
Developing Dictyostelium cells form large aggregation
streams that break up into groups of 0.2 × 105 to
1 × 105 cells. Each group then becomes a fruiting body.
smlA cells oversecrete an unknown factor that causes
aggregation streams to break up into groups of
~5 × 103 cells and thus form very small fruiting
bodies. We have purified the counting factor and find that it behaves
as a complex of polypeptides with an effective molecular mass of 450 kD. One of the polypeptides is a 40-kD hydrophilic protein we have
named countin. In transformants with a disrupted countin gene,
there is no detectable secretion of counting factor, and the
aggregation streams do not break up, resulting in huge (up to
2 × 105 cell) fruiting bodies.
[Key Words: Cell number; cell counting; tissue size; diffusion]
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