.gif?ad=16839&adview=true)
![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 13, No. 18, pp. 2425-2438, September 15, 1999
1 European Molecular Biology Laboratory, D-69117
Heidelberg, Germany; 2 Department of Biology, Williams
College, Williamstown, Massachusetts 01267 USA
The characterization of a novel yeast-splicing factor, Luc7p, is
presented. The LUC7 gene was identified by a mutation that causes lethality in a yeast strain lacking the nuclear cap-binding complex (CBC). Luc7p is similar in sequence to metazoan proteins that
have arginine-serine and arginine-glutamic acid repeat sequences characteristic of a family of splicing factors. We show that Luc7p is a
component of yeast U1 snRNP and is essential for vegetative growth. The
composition of yeast U1 snRNP is altered in luc7 mutant strains. Extracts of these strains are unable to support any of the
defined steps of splicing unless recombinant Luc7p is added. Although
the in vivo defect in splicing wild-type reporter introns in a
luc7 mutant strain is comparatively mild, splicing of introns with nonconsensus 5' splice site or branchpoint sequences is more defective in the mutant strain than in wild-type strains. By use of
reporters that have two competing 5' splice sites, a loss of efficient splicing to the cap proximal splice site is observed in
luc7 cells, analogous to the defect seen in strains lacking CBC. CBC can be coprecipitated with U1 snRNP from wild-type, but not
from luc7, yeast strains. These data suggest that the loss of
Luc7p disrupts U1 snRNP-CBC interaction, and that this interaction contributes to normal 5' splice site recognition.
[Key Words: Pre-mRNA processing; U1 snRNP; 5' splice site recognition; cap-binding complex; alternative splicing]
CiteULike 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  S. Laubinger, T. Sachsenberg, G. Zeller, W. Busch, J. U. Lohmann, G. Ratsch, and D. Weigel Dual roles of the nuclear cap-binding complex and SERRATE in pre-mRNA splicing and microRNA processing in Arabidopsis thaliana PNAS, June 24, 2008; 105(25): 8795 - 8800. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. D. Ellis, D. Lleres, M. Denegri, A. I. Lamond, and J. F. Caceres Spatial mapping of splicing factor complexes involved in exon and intron definition J. Cell Biol., June 16, 2008; 181(6): 921 - 934. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. M. Howell, J. M. Jones, S. K. Bergren, L. Li, A. C. Billi, M. R. Avenarius, and M. H. Meisler Evidence for a direct role of the disease modifier SCNM1 in splicing Hum. Mol. Genet., October 15, 2007; 16(20): 2506 - 2516. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Puig, E. Bragado-Nilsson, T. Koski, and B. Seraphin The U1 snRNP-associated factor Luc7p affects 5' splice site selection in yeast and human Nucleic Acids Res., September 27, 2007; 35(17): 5874 - 5885. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Qiu, F. Cheng, and D. Pintel Distance-Dependent Processing of Adeno-Associated Virus Type 5 RNA Is Controlled by 5' Exon Definition J. Virol., August 1, 2007; 81(15): 7974 - 7984. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-I G. Chen, R. E. Moore, H. Y. Ge, M. K. Young, T. D. Lee, and S. W. Stevens Proteomic analysis of in vivo-assembled pre-mRNA splicing complexes expands the catalog of participating factors Nucleic Acids Res., June 9, 2007; 35(12): 3928 - 3944. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Narvaiza, O. Aparicio, M. Vera, N. Razquin, S. Bortolanza, J. Prieto, and P. Fortes Effect of Adenovirus-Mediated RNA Interference on Endogenous MicroRNAs in a Mouse Model of Multidrug Resistance Protein 2 Gene Silencing J. Virol., December 15, 2006; 80(24): 12236 - 12247. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Aparicio, N. Razquin, M. Zaratiegui, I. Narvaiza, and P. Fortes Adenovirus Virus-Associated RNA Is Processed to Functional Interfering RNAs Involved in Virus Production J. Virol., February 1, 2006; 80(3): 1376 - 1384. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Cazalla, K. Newton, and J. F. Caceres A Novel SR-Related Protein Is Required for the Second Step of Pre-mRNA Splicing Mol. Cell. Biol., April 15, 2005; 25(8): 2969 - 2980. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Collins and D. Penny Complex Spliceosomal Organization Ancestral to Extant Eukaryotes Mol. Biol. Evol., April 1, 2005; 22(4): 1053 - 1066. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Sakashita, S. Tatsumi, D. Werner, H. Endo, and A. Mayeda Human RNPS1 and Its Associated Factors: a Versatile Alternative Pre-mRNA Splicing Regulator In Vivo Mol. Cell. Biol., February 1, 2004; 24(3): 1174 - 1187. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. W. Murphy, B. L. Olson, and P. G. Siliciano The Yeast Splicing Factor Prp40p Contains Functional Leucine-Rich Nuclear Export Signals That Are Essential for Splicing Genetics, January 1, 2004; 166(1): 53 - 65. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. McKendrick, E. Thompson, J. Ferreira, S. J. Morley, and J. D. Lewis Interaction of Eukaryotic Translation Initiation Factor 4G with the Nuclear Cap-Binding Complex Provides a Link between Nuclear and Cytoplasmic Functions of the m7 Guanosine Cap Mol. Cell. Biol., June 1, 2001; 21(11): 3632 - 3641. [Abstract] [Full Text]
|
|
|
|
|
|
I. C. Eperon, O. V. Makarova, A. Mayeda, S. H. Munroe, J. F. Cáceres, D. G. Hayward, and A. R. Krainer Selection of Alternative 5' Splice Sites: Role of U1 snRNP and Models for the Antagonistic Effects of SF2/ASF and hnRNP A1 Mol. Cell. Biol., November 15, 2000; 20(22): 8303 - 8318. [Abstract] [Full Text]
|
|
|
|
|
|
F. Del Gatto-Konczak, C. F. Bourgeois, C. Le Guiner, L. Kister, M.-C. Gesnel, J. Stévenin, and R. Breathnach The RNA-Binding Protein TIA-1 Is a Novel Mammalian Splicing Regulator Acting through Intron Sequences Adjacent to a 5' Splice Site Mol. Cell. Biol., September 1, 2000; 20(17): 6287 - 6299. [Abstract] [Full Text]
|
|
|
|
|
|
S. M. Mount and H. K. Salz Pre-messenger RNA Processing Factors in the Drosophila Genome J. Cell Biol., July 24, 2000; 150(2): F37 - F44. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. C. Shen, T. Stage-Zimmermann, P. Chui, and P. A. Silver 7The Yeast mRNA-binding Protein Npl3p Interacts with the Cap-binding Complex J. Biol. Chem., July 28, 2000; 275(31): 23718 - 23724. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Awasthi, R. Palmer, M. Castro, C. D. Mobarak, and S. W. Ruby New Roles for the Snp1 and Exo84 Proteins in Yeast Pre-mRNA Splicing J. Biol. Chem., August 10, 2001; 276(33): 31004 - 31015. [Abstract] [Full Text] [PDF]
|
|
