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Vol. 13, No. 22, pp. 2940-2945, November 15, 1999
Department of Genetics, Harvard Medical School,
Boston, Massachusetts 02115 USA
The SAGA complex of Saccharomyces cerevisiae is required
for the transcription of many RNA polymerase II-dependent genes. Previous studies have demonstrated that SAGA possesses histone acetyltransferase activity, catalyzed by the SAGA component Gcn5. However, the transcription of many genes, although SAGA dependent, is
Gcn5 independent, suggesting the existence of distinct SAGA activities.
We have studied the in vivo role of two other SAGA components, Spt3 and
Spt20, at the well-characterized GAL1 promoter. Our results
demonstrate that both Spt3 and Spt20 are required for the binding of
TATA-binding protein but not of the activator Gal4 and that this role
is Gcn5 independent. These results suggest a coactivator role for Spt3
and Spt20 in the recruitment of TBP.
[Key Words: SAGA complex; S. cerevisiae; transcription; Spt3; TBP]
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