![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 13, No. 7, pp. 841-850, April 1, 1999
Department of Cell Biology, Harvard Medical School,
Boston, Massachusetts 02115 USA
The spliceosome catalyzes pre-mRNA splicing in two steps. After
catalytic step I, a major remodeling of the spliceosome occurs to
establish the active site for step II. Here, we report the isolation of
a cDNA encoding hSlu7, the human homolog of the yeast second step
splicing factor Slu7. We show that hSlu7 associates with the
spliceosome late in the splicing pathway, but at a stage prior to
recognition of the 3' splice site for step II. In the absence of
hSlu7, splicing is stalled between the catalytic steps in a novel
complex, the C
hSlu7 complex. We provide evidence that this
complex differs significantly in structure from the known spliceosomal
complexes, yet is a functional intermediate between the catalytic steps
of splicing. Together, our observations indicate that hSlu7 is required
for a structural alteration of the spliceosome prior to the
establishment of the catalytically active spliceosome for step II.
[Key Words: Pre-mRNA splicing; splicing factor; catalytic step II; spliceosome; hSlu7]
CiteULike 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  M. Alberstein, M. Amit, K. Vaknin, A. O'Donnell, C. Farhy, Y. Lerenthal, N. Shomron, O. Shaham, A. D. Sharrocks, R. Ashery-Padan, et al. Regulation of transcription of the RNA splicing factor hSlu7 by Elk-1 and Sp1 affects alternative splicing RNA, November 1, 2007; 13(11): 1988 - 1999. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Wang, J. W. Park, J. L. Wang, and R. J. Patterson Immunoprecipitation of spliceosomal RNAs by antisera to galectin-1 and galectin-3 Nucleic Acids Res., October 6, 2006; (2006) gkl673v3. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. K. Clay and T. Nelson The Recessive Epigenetic swellmap Mutation Affects the Expression of Two Step II Splicing Factors Required for the Transcription of the Cell Proliferation Gene STRUWWELPETER and for the Timing of Cell Cycle Arrest in the Arabidopsis Leaf PLANT CELL, July 1, 2005; 17(7): 1994 - 2008. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Cazalla, K. Newton, and J. F. Caceres A Novel SR-Related Protein Is Required for the Second Step of Pre-mRNA Splicing Mol. Cell. Biol., April 15, 2005; 25(8): 2969 - 2980. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Shomron, M. Alberstein, M. Reznik, and G. Ast Stress alters the subcellular distribution of hSlu7 and thus modulates alternative splicing J. Cell Sci., March 15, 2005; 118(6): 1151 - 1159. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Shomron, M. Reznik, and G. Ast Splicing Factor hSlu7 Contains a Unique Functional Domain Required to Retain the Protein within the Nucleus Mol. Biol. Cell, August 1, 2004; 15(8): 3782 - 3795. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Shomron, H. Malca, I. Vig, and G. Ast Reversible inhibition of the second step of splicing suggests a possible role of zinc in the second step of splicing Nucleic Acids Res., October 1, 2002; 30(19): 4127 - 4137. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. C. Dietrich, M. J. Peris, A. S. Seyboldt, and R. A. Padgett Role of the 3' Splice Site in U12-Dependent Intron Splicing Mol. Cell. Biol., March 15, 2001; 21(6): 1942 - 1952. [Abstract] [Full Text]
|
|
|
|
|
|
N. F. Kaufer and J. Potashkin SURVEY AND SUMMARY: Analysis of the splicing machinery in fission yeast: a comparison with budding yeast and mammals Nucleic Acids Res., August 15, 2000; 28(16): 3003 - 3010. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Jiang, D. S. Horowitz, and R.-M. Xu Crystal structure of the functional domain of the splicing factor Prp18 PNAS, March 28, 2000; 97(7): 3022 - 3027. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Chen, K. Anderson, and M. J. Moore Evidence for a linear search in bimolecular 3' splice site AG selection PNAS, January 18, 2000; 97(2): 593 - 598. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. L. Chew, H.-X. Liu, A. Mayeda, and A. R. Krainer Evidence for the function of an exonic splicing enhancer after the first catalytic step of pre-mRNA splicing PNAS, September 14, 1999; 96(19): 10655 - 10660. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. A. Collins and C. Guthrie Allele-specific genetic interactions between Prp8 and RNA active site residues suggest a function for Prp8 at the catalytic core of the spliceosome Genes & Dev., August 1, 1999; 13(15): 1970 - 1982. [Abstract] [Full Text]
|
|
