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Vol. 14, No. 15, pp. 1945-1957, August 1, 2000
1 Umeå Center for Molecular Pathogenesis (UCMP), Umeå
University, S-90187 Umeå, Sweden; 2 Department of Zoology,
Stockholm University, S-10691 Stockholm, Sweden
Many developmental and physiological responses rely on the selective
translocation of transcriptional regulators in and out of the nucleus
through the nuclear pores. Here we describe the Drosophila gene
members only (mbo) encoding a nucleoporin homologous to
the mammalian Nup88. The phenotypes of mbo mutants and
mbo expression during development are cell specific, indicating
that the nuclear import capacity of cells is differentially regulated. Using inducible assays for nucleocytoplasmic trafficking we show that
mRNA export and classic NLS-mediated protein import are unaffected in
mbo mutants. Instead, mbo is selectively required for
the nuclear import of the yeast transcription factor GAL4 in a subset
of the larval tissues. We have identified the first endogenous targets of the mbo nuclear import pathway in the Rel proteins Dorsal
and Dif. In mbo mutants the upstream signaling events leading
to the degradation of the I
B homolog Cactus are functional, but
Dorsal and Dif remain cytoplasmic and the larval immune response is not activated in response to infection. Our results demonstrate that distinct nuclear import events require different nucleoporins in vivo
and suggest a regulatory role for mbo in signal transduction.
[Key Words: Nuclear import; Rel family; Nup88; innate immunity; tracheal branch fusion]
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