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Vol. 14, No. 17, pp. 2192-2205, September 1, 2000
Department of Genetics, University of Bayreuth, 95440 Bayreuth, Germany
Drosophila Pimples (PIM) and Three rows (THR) are required for
sister chromatid separation in mitosis. PIM accumulates during interphase and is degraded rapidly during mitosis. This degradation is
dependent on a destruction box similar to that of B-type cyclins. Nondegradable PIM with a mutant destruction box can rescue sister chromatid separation in pim mutants but only when expressed at low levels. Higher levels of nondegradable PIM, as well as
overexpression of wild-type PIM, inhibit sister chromatid separation.
Moreover, cells arrested in mitosis before sister chromatid separation
(by colcemid or by mutations in fizzy/CDC20) fail to degrade
PIM. Thus, although not related by primary sequence, PIM has intriguing functional similarities to the securin proteins of budding yeast, fission yeast, and vertebrates. Whereas these securins are known to
form a complex with separins, we show that PIM associates in vivo with
THR, which does not contain the conserved separin domain.
[Key Words: Mitosis; sister chromatid separation; securin; separin; pimples; three rows]
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