![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 14, No. 21, pp. 2737-2744, November 1, 2000
1 Department of Biochemistry and Molecular Biology,
University of Texas M.D. Anderson Cancer Center, Houston, Texas 77030, USA; 2 Division of Molecular Biology and Genetics, Department
of Oncological Sciences, University of Utah Health Science Center,
Salt Lake City, Utah 84132, USA
Ssn6-Tup1 regulates multiple genes in yeast, providing a paradigm
for corepressor functions. Tup1 interacts directly with histones H3 and
H4, and mutation of these histones synergistically compromises
Ssn6-Tup1-mediated repression. In vitro, Tup1 interacts preferentially
with underacetylated isoforms of H3 and H4, suggesting that histone
acetylation may modulate Tup1 functions in vivo. Here we report that
histone hyperacetylation caused by combined mutations in genes encoding
the histone deacetylases (HDACs) Rpd3, Hos1, and Hos2 abolishes
Ssn6-Tup1 repression. Unlike HDAC mutations that do not affect
repression, this combination of mutations causes concomitant
hyperacetylation of both H3 and H4. Strikingly, two of these class I
HDACs interact physically with Ssn6-Tup1. These findings suggest that
Ssn6-Tup1 actively recruits deacetylase activities to deacetylate
adjacent nucleosomes and promote Tup1-histone interactions.
[Key Words: Chromatin; transcription; nucleosome; yeast; acetylation]
CiteULike 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  C. A. Martinez and D. N. Arnosti Spreading of a Corepressor Linked to Action of Long-Range Repressor Hairy Mol. Cell. Biol., April 15, 2008; 28(8): 2792 - 2802. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. R. Barrales, J. Jimenez, and J. I. Ibeas Identification of Novel Activation Mechanisms for FLO11 Regulation in Saccharomyces cerevisiae Genetics, January 1, 2008; 178(1): 145 - 156. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. M. Sharma, R. S. Tomar, A. E. Dempsey, and J. C. Reese Histone Deacetylases RPD3 and HOS2 Regulate the Transcriptional Activation of DNA Damage-Inducible Genes Mol. Cell. Biol., April 15, 2007; 27(8): 3199 - 3210. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Sertil, A. Vemula, S. L. Salmon, R. H. Morse, and C. V. Lowry Direct Role for the Rpd3 Complex in Transcriptional Induction of the Anaerobic DAN/TIR Genes in Yeast Mol. Cell. Biol., March 15, 2007; 27(6): 2037 - 2047. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Fagerstrom-Billai, M. Durand-Dubief, K. Ekwall, and A. P. H. Wright Individual Subunits of the Ssn6-Tup11/12 Corepressor Are Selectively Required for Repression of Different Target Genes Mol. Cell. Biol., February 1, 2007; 27(3): 1069 - 1082. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Hirota, C. S. Hoffman, and K. Ohta Reciprocal Nuclear Shuttling of Two Antagonizing Zn Finger Proteins Modulates Tup Family Corepressor Function To Repress Chromatin Remodeling Eukaryot. Cell, December 1, 2006; 5(12): 1980 - 1989. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Morohashi, Y. Yamamoto, S. Kuwana, W. Morita, H. Shindo, A. P. Mitchell, and M. Shimizu Effect of Sequence-Directed Nucleosome Disruption on Cell-Type-Specific Repression by {alpha}2/Mcm1 in the Yeast Genome Eukaryot. Cell, November 1, 2006; 5(11): 1925 - 1933. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. MacPherson, M. Larochelle, and B. Turcotte A Fungal Family of Transcriptional Regulators: the Zinc Cluster Proteins Microbiol. Mol. Biol. Rev., September 1, 2006; 70(3): 583 - 604. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. S. J. Davies and J. Rine A Role for Sterol Levels in Oxygen Sensing in Saccharomyces cerevisiae Genetics, September 1, 2006; 174(1): 191 - 201. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. G. Klinkenberg, T. Webb, and R. S. Zitomer Synergy among Differentially Regulated Repressors of the Ribonucleotide Diphosphate Reductase Genes of Saccharomyces cerevisiae. Eukaryot. Cell, July 1, 2006; 5(7): 1007 - 1017. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. J. C. van Oevelen, H. A. A. M. van Teeffelen, F. J. van Werven, and H. Th. M. Timmers Snf1p-dependent Spt-Ada-Gcn5-acetyltransferase (SAGA) Recruitment and Chromatin Remodeling Activities on the HXT2 and HXT4 Promoters J. Biol. Chem., February 17, 2006; 281(7): 4523 - 4531. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-j. Kim, M. J. Swanson, H. Qiu, C. K. Govind, and A. G. Hinnebusch Activator Gcn4p and Cyc8p/Tup1p Are Interdependent for Promoter Occupancy at ARG1 In Vivo Mol. Cell. Biol., December 15, 2005; 25(24): 11171 - 11183. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Zhang and J. C. Reese Molecular Genetic Analysis of the Yeast Repressor Rfx1/Crt1 Reveals a Novel Two-Step Regulatory Mechanism Mol. Cell. Biol., September 1, 2005; 25(17): 7399 - 7411. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X.-J. Yang and S. Gregoire Class II Histone Deacetylases: from Sequence to Function, Regulation, and Clinical Implication Mol. Cell. Biol., April 15, 2005; 25(8): 2873 - 2884. [Full Text] [PDF]
|
|
|
|
|
|
T. Macfarlan, S. Kutney, B. Altman, R. Montross, J. Yu, and D. Chakravarti Human THAP7 Is a Chromatin-associated, Histone Tail-binding Protein That Represses Transcription via Recruitment of HDAC3 and Nuclear Hormone Receptor Corepressor J. Biol. Chem., February 25, 2005; 280(8): 7346 - 7358. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Fagerstrom-Billai and A. P. H. Wright Functional Comparison of the Tup11 and Tup12 Transcriptional Corepressors in Fission Yeast Mol. Cell. Biol., January 15, 2005; 25(2): 716 - 727. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Toleman, A. J. Paterson, T. R. Whisenhunt, and J. E. Kudlow Characterization of the Histone Acetyltransferase (HAT) Domain of a Bifunctional Protein with Activable O-GlcNAcase and HAT Activities J. Biol. Chem., December 17, 2004; 279(51): 53665 - 53673. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. R. Mathias, S. E. Hanlon, R. A. O'Flanagan, A. M. Sengupta, and A. K. Vershon Repression of the yeast HO gene by the MAT{alpha}2 and MATa1 homeodomain proteins Nucleic Acids Res., December 14, 2004; 32(22): 6469 - 6478. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Sabet, S. Volo, C. Yu, J. P. Madigan, and R. H. Morse Genome-Wide Analysis of the Relationship between Transcriptional Regulation by Rpd3p and the Histone H3 and H4 Amino Termini in Budding Yeast Mol. Cell. Biol., October 15, 2004; 24(20): 8823 - 8833. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. A. Simms, E. C. Miller, N. P. Buisson, N. Jambunathan, and D. Donze The Saccharomyces cerevisiae TRT2 tRNAThr gene upstream of STE6 is a barrier to repression in MAT{alpha} cells and exerts a potential tRNA position effect in MATa cells Nucleic Acids Res., September 30, 2004; 32(17): 5206 - 5213. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Zhang and J. C. Reese Redundant Mechanisms Are Used by Ssn6-Tup1 in Repressing Chromosomal Gene Transcription in Saccharomyces cerevisiae J. Biol. Chem., September 17, 2004; 279(38): 39240 - 39250. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Puig, M. Lau, and D. J. Thiele Cti6 Is an Rpd3-Sin3 Histone Deacetylase-associated Protein Required for Growth under Iron-limiting Conditions in Saccharomyces cerevisiae J. Biol. Chem., July 16, 2004; 279(29): 30298 - 30306. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. G. Aparicio, C. J. Viggiani, D. G. Gibson, and O. M. Aparicio The Rpd3-Sin3 Histone Deacetylase Regulates Replication Timing and Enables Intra-S Origin Control in Saccharomyces cerevisiae Mol. Cell. Biol., June 1, 2004; 24(11): 4769 - 4780. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Boukaba, E. I. Georgieva, F. A. Myers, A. W. Thorne, G. Lopez-Rodas, C. Crane-Robinson, and L. Franco A Short-range Gradient of Histone H3 Acetylation and Tup1p Redistribution at the Promoter of the Saccharomyces cerevisiae SUC2 Gene J. Biol. Chem., February 27, 2004; 279(9): 7678 - 7684. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Hirota, T. Hasemi, T. Yamada, K.-i. Mizuno, C. S. Hoffman, T. Shibata, and K. Ohta Fission yeast global repressors regulate the specificity of chromatin alteration in response to distinct environmental stresses Nucleic Acids Res., February 3, 2004; 32(2): 855 - 862. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. K. Davie, D. G. Edmondson, C. B. Coco, and S. Y. R. Dent Tup1-Ssn6 Interacts with Multiple Class I Histone Deacetylases in Vivo J. Biol. Chem., December 12, 2003; 278(50): 50158 - 50162. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Hirota, C. S. Hoffman, T. Shibata, and K. Ohta Fission Yeast Tup1-Like Repressors Repress Chromatin Remodeling at the fbp1+ Promoter and the ade6-M26 Recombination Hotspot Genetics, October 1, 2003; 165(2): 505 - 515. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Trojer, E. M. Brandtner, G. Brosch, P. Loidl, J. Galehr, R. Linzmaier, H. Haas, K. Mair, M. Tribus, and S. Graessle Histone deacetylases in fungi: novel members, new facts Nucleic Acids Res., July 15, 2003; 31(14): 3971 - 3981. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Mukai, J. K. Davie, and S. Y. R. Dent Physical and Functional Interaction of the Yeast Corepressor Tup1 with mRNA 5'-Triphosphatase J. Biol. Chem., May 23, 2003; 278(21): 18895 - 18901. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Sabet, F. Tong, J. P. Madigan, S. Volo, M. M. Smith, and R. H. Morse Global and specific transcriptional repression by the histone H3 amino terminus in yeast PNAS, April 1, 2003; 100(7): 4084 - 4089. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Zhang, M. Barboric, T. K. Blackwell, and B. M. Peterlin A model of repression: CTD analogs and PIE-1 inhibit transcriptional elongation by P-TEFb Genes & Dev., March 15, 2003; 17(6): 748 - 758. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Li, L. Howe, S. Anderson, J. R. Yates III, and J. L. Workman The Set2 Histone Methyltransferase Functions through the Phosphorylated Carboxyl-terminal Domain of RNA Polymerase II J. Biol. Chem., March 7, 2003; 278(11): 8897 - 8903. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. E. Olson, J. S. Dasen, B. Gun Ju, J. Tollkuhn, and M. G. Rosenfeld Paired-like Repression/Activation in Pituitary Development Recent Prog. Horm. Res., January 1, 2003; 58(1): 249 - 261. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Cuperus and D. Shore Restoration of Silencing in Saccharomyces cerevisiae by Tethering of a Novel Sir2-Interacting Protein, Esc8 Genetics, October 1, 2002; 162(2): 633 - 645. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Greenall, A. P. Hadcroft, P. Malakasi, N. Jones, B. A. Morgan, C. S. Hoffman, and S. K. Whitehall Role of Fission Yeast Tup1-like Repressors and Prr1 Transcription Factor in Response to Salt Stress Mol. Biol. Cell, September 1, 2002; 13(9): 2977 - 2989. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Zhang, U. Varanasi, and R. J. Trumbly Functional Dissection of the Global Repressor Tup1 in Yeast: Dominant Role of the C-Terminal Repression Domain Genetics, July 1, 2002; 161(3): 957 - 969. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Zhao, S. R. Lockhart, K. Daniels, and D. R. Soll Roles of TUP1 in Switching, Phase Maintenance, and Phase-Specific Gene Expression in Candida albicans Eukaryot. Cell, June 1, 2002; 1(3): 353 - 365. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. D. Norris, D. Fan, A. Sherk, and D. P. McDonnell A Negative Coregulator for the Human ER Mol. Endocrinol., March 1, 2002; 16(3): 459 - 468. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. K. Davie, R. J. Trumbly, and S. Y. R. Dent Histone-Dependent Association of Tup1-Ssn6 with Repressed Genes In Vivo Mol. Cell. Biol., February 1, 2002; 22(3): 693 - 703. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Zhang and S. W. Emmons Caenorhabditis elegans unc-37/groucho Interacts Genetically With Components of the Transcriptional Mediator Complex Genetics, February 1, 2002; 160(2): 799 - 803. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. S. Dasen, J.-P. M. Barbera, T. S. Herman, S. O' Connell, L. Olson, B. Ju, J. Tollkuhn, S. H. Baek, D. W. Rose, and M. G. Rosenfeld Temporal regulation of a paired-like homeodomain repressor/TLE corepressor complex and a related activator is required for pituitary organogenesis Genes & Dev., December 1, 2001; 15(23): 3193 - 3207. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. J. Courey and S. Jia Transcriptional repression: the long and the short of it Genes & Dev., November 1, 2001; 15(21): 2786 - 2796. [Full Text] [PDF]
|
|
|
|
|
|
S. Poux, R. Melfi, and V. Pirrotta Establishment of Polycomb silencing requires a transient interaction between PC and ESC Genes & Dev., October 1, 2001; 15(19): 2509 - 2514. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. A. Stafford and R. H. Morse GCN5 Dependence of Chromatin Remodeling and Transcriptional Activation by the GAL4 and VP16 Activation Domains in Budding Yeast Mol. Cell. Biol., July 15, 2001; 21(14): 4568 - 4578. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Yu, P. Li, R. G. Roeder, and Z. Wang Inhibition of Androgen Receptor-Mediated Transcription by Amino-Terminal Enhancer of split Mol. Cell. Biol., July 15, 2001; 21(14): 4614 - 4625. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. S. Yochum and D. E. Ayer Pf1, a Novel PHD Zinc Finger Protein That Links the TLE Corepressor to the mSin3A-Histone Deacetylase Complex Mol. Cell. Biol., July 1, 2001; 21(13): 4110 - 4118. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Baidyaroy, G. Brosch, J.-h. Ahn, S. Graessle, S. Wegener, N. J. Tonukari, O. Caballero, P. Loidl, and J. D. Walton A Gene Related to Yeast HOS2 Histone Deacetylase Affects Extracellular Depolymerase Expression and Virulence in a Plant Pathogenic Fungus PLANT CELL, July 1, 2001; 13(7): 1609 - 1624. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Deckert and K. Struhl Histone Acetylation at Promoters Is Differentially Affected by Specific Activators and Repressors Mol. Cell. Biol., April 15, 2001; 21(8): 2726 - 2735. [Abstract] [Full Text]
|
|
|
|
|
|
L. N. R. a. J. Rine Conversion of a gene-specific repressor to a regional silencer Genes & Dev., April 15, 2001; 15(8): 955 - 967. [Abstract] [Full Text]
|
|
|
|
|
|
Z. Zaman, A. Z. Ansari, S. S. Koh, R. Young, and M. Ptashne Interaction of a transcriptional repressor with the RNA polymerase II holoenzyme plays a crucial role in repression PNAS, February 27, 2001; 98(5): 2550 - 2554. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. R. Bone and S. Y. Roth Recruitment of the Yeast Tup1p-Ssn6p Repressor Is Associated with Localized Decreases in Histone Acetylation J. Biol. Chem., January 12, 2001; 276(3): 1808 - 1813. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Li and J. C. Reese Ssn6-Tup1 Regulates RNR3 by Positioning Nucleosomes and Affecting the Chromatin Structure at the Upstream Repression Sequence J. Biol. Chem., August 31, 2001; 276(36): 33788 - 33797. [Abstract] [Full Text] [PDF]
|
|
