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Vol. 14, No. 22, pp. 2906-2917, November 15, 2000

RESEARCH PAPER
Drosophila homologs of transcriptional mediator complex subunits are required for adult cell and segment identity specification

Muriel Boube,1 Christian Faucher, Laurent Joulia, David L. Cribbs,2 and Henri-Marc Bourbon

Centre de Biologie du Développement-CNRS, 31062 Toulouse CEDEX 04, France

The origins of specificity in gene expression are a central concern in understanding developmental control. Mediator protein complexes regulate transcriptional initiation, acting as modular adaptors linking specific transcription factors to core RNA polymerase II. Here, we identified the Drosophila homologs of 23 human mediator genes and mutations of two, dTRAP240 and of dTRAP80 (the putative fly homolog of yeast SRB4). Clonal analysis indicates a general role for dTRAP80 necessary for cell viability. The dTRAP240 gene is also essential, but cells lacking its function are viable and proliferate normally. Clones reveal localized developmental activities including a sex comb cell identity function. This contrasts with the ubiquitous nuclear accumulation of dTRAP240 protein in imaginal discs. Synergistic genetic interactions support shared developmental cell and segment identity functions of dTRAP240 and dTRAP80, potentially within a common complex. Further, they identify the homeotic Sex combs reduced product, required for the same cell/tissue identities, as a functional partner of these mediator proteins.

[Key Words: Mediator; SRB; transcription; cell identity; segment identity; homeotic; development]

1 Present address: Department of Molecular and Cellular Biology, CID-CSIC, c/ Jordi Girona 18-26, Barcelona 08034, Spain.

2 Corresponding author.

GENES & DEVELOPMENT 14:2906-2917 © 2000 by Cold Spring Harbor Laboratory Press  ISSN 0890-9369/00 $5.00
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