.gif?ad=16248&adview=true)
![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 14, No. 23, pp. 2976-2988, December 1, 2000
1 Division of Molecular Genetics, Department of
Biochemistry, University of Oxford, OX1 3QU, UK;
2 Laboratoire de Microbiologie et Génétique
Moléculaire, Centre National de la Recherche Scientifique,
Toulouse, France
In bacteria with circular chromosomes, homologous recombination can
generate chromosome dimers that cannot be segregated to daughter cells
at cell division. Xer site-specific recombination at dif, a
28-bp site located in the replication terminus region of the
chromosome, converts dimers to monomers through the sequential action
of the XerC and XerD recombinases. Chromosome dimer resolution requires
that dif is positioned correctly in the chromosome, and the
activity of FtsK, a septum-located protein that coordinates cell
division with chromosome segregation. Here, we show that cycles of
XerC-mediated strand exchanges form and resolve Holliday junction
intermediates back to substrate irrespective of whether conditions
support a complete recombination reaction. The C-terminal domain of
FtsK is sufficient to activate the exchange of the second pair of
strands by XerD, allowing both intra- and intermolecular recombination
reactions to go to completion. Proper positioning of dif in the
chromosome and of FtsK at the septum is required to sense the
multimeric state of newly replicated chromosomes and restrict complete
Xer reactions to dimeric chromosomes.
[Key Words: Xer recombination; Holliday junction; chromosome segregation; FtsK]
CiteULike 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  A. E. Bloor and R. M. Cranenburgh An Efficient Method of Selectable Marker Gene Excision by Xer Recombination for Gene Replacement in Bacterial Chromosomes Appl. Envir. Microbiol., April 1, 2006; 72(4): 2520 - 2525. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Bui, J. Ramiscal, S. Trigueros, J. S. Newmark, A. Do, D. J. Sherratt, and M. E. Tolmasky Differences in Resolution of mwr-Containing Plasmid Dimers Mediated by the Klebsiella pneumoniae and Escherichia coli XerC Recombinases: Potential Implications in Dissemination of Antibiotic Resistance Genes. J. Bacteriol., April 1, 2006; 188(8): 2812 - 2820. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Kobryn, A. B. Burgin, and G. Chaconas Uncoupling the Chemical Steps of Telomere Resolution by ResT J. Biol. Chem., July 22, 2005; 280(29): 26788 - 26795. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Espeli, C. Lee, and K. J. Marians A Physical and Functional Interaction between Escherichia coli FtsK and Topoisomerase IV J. Biol. Chem., November 7, 2003; 278(45): 44639 - 44644. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Dohmann, B. Strommenger, K. Stevenson, L. de Juan, J. Stratmann, V. Kapur, T. J. Bull, and G.-F. Gerlach Characterization of Genetic Differences between Mycobacterium avium subsp. paratuberculosis Type I and Type II Isolates J. Clin. Microbiol., November 1, 2003; 41(11): 5215 - 5223. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. J. Dery, B. Soballe, M. S. L. Witherspoon, D. Bui, R. Koch, D. J. Sherratt, and M. E. Tolmasky The Aminoglycoside 6'-N-Acetyltransferase Type Ib Encoded by Tn1331 Is Evenly Distributed within the Cell's Cytoplasm Antimicrob. Agents Chemother., September 1, 2003; 47(9): 2897 - 2902. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. GE, Y.-Y. E. CHUANG, S. ZHAO, J. J. TEMENAK, and W.-M. CHING Genomic Studies of Rickettsia prowazekii Virulent and Avirulent Strains Ann. N.Y. Acad. Sci., June 1, 2003; 990(1): 671 - 677. [Full Text] [PDF]
|
|
|
|
|
|
J. Corre and J.-M. Louarn Evidence from Terminal Recombination Gradients that FtsK Uses Replichore Polarity To Control Chromosome Terminus Positioning at Division in Escherichia coli J. Bacteriol., July 15, 2002; 184(14): 3801 - 3807. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Pham, K. J. Dery, D. J. Sherratt, and M. E. Tolmasky Osmoregulation of Dimer Resolution at the Plasmid pJHCMW1 mwr Locus by Escherichia coli XerCD Recombination J. Bacteriol., March 15, 2002; 184(6): 1607 - 1616. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. M. Cox Historical overview: Searching for replication help in all of the rec places PNAS, July 17, 2001; 98(15): 8173 - 8180. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F.-X. Barre, B. Soballe, B. Michel, M. Aroyo, M. Robertson, and D. Sherratt Circles: The replication-recombination-chromosome segregation connection PNAS, July 17, 2001; 98(15): 8189 - 8195. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Kuzminov DNA replication meets genetic exchange: Chromosomal damage and its repair by homologous recombination PNAS, July 17, 2001; 98(15): 8461 - 8468. [Abstract] [Full Text] [PDF]
|
|
