Genes and Development

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kasahara, M.
Right arrow Articles by Kogoma, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kasahara, M.
Right arrow Articles by Kogoma, T.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Vol. 14, No. 3, pp. 360-365, February 1, 2000

RESEARCH PAPER
RecA protein-dependent R-loop formation in vitro

Megumi Kasahara,1 Jennifer A. Clikeman, David B. Bates,2,3 and Tokio Kogoma4

Department of Molecular Genetics and Microbiology, University of New Mexico Health Sciences Center, Albuquerque, New Mexico 87131 USA

The RecA protein of Escherichia coli, which has crucial roles in homologous recombination, DNA damage repair, induction of the SOS response, and SOS mutagenesis, was found to catalyze assimilation of complementary RNA into a homologous region of a DNA duplex (R-loop). The reaction strictly requires a region of mismatch in the duplex, which may serve as a nucleation site for RecA protein polymerization. The optimum conditions for the assimilation reaction resemble those for the previously studied RecA protein-catalyzed homologous pairing and strand exchange reaction between two DNA molecules. Our finding lends strong support to the proposal that RecA protein-catalyzed assimilation of a transcript into duplex DNA results in formation of an R-loop at certain regions of the chromosome and that, when stabilized, the R-loop can serve as an origin of chromosome replication.

[Key Words: RecA protein; R-loop; cSDR; RNase H; DNA replication]

Present addresses: 1Department of Biology, Hyogo University of Teacher Education, Shimokume, Yashiro, Hyogo 673-1494, Japan; 2Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138 USA.

3 Corresponding author.

4 Dr. Tokio Kogoma passed away on October 9, 1997, and the authors dedicate this paper to him.

GENES & DEVELOPMENT 14:360-365 © 2000 by Cold Spring Harbor Laboratory Press  ISSN 0890-9369/00 $5.00
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
S. Kaneko, C. Chu, A. J. Shatkin, and J. L. Manley
Human capping enzyme promotes formation of transcriptional R loops in vitro
PNAS, November 6, 2007; 104(45): 17620 - 17625.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
S. J. Sandler
Requirements for Replication Restart Proteins During Constitutive Stable DNA Replication in Escherichia coli K-12
Genetics, April 1, 2005; 169(4): 1799 - 1806.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
P. E. Boehmer
RNA binding and R-loop formation by the herpes simplex virus type-1 single-stranded DNA-binding protein (ICP8)
Nucleic Acids Res., August 25, 2004; 32(15): 4576 - 4584.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
O. G. Rossler, A. Straka, and H. Stahl
Rearrangement of structured RNA via branch migration structures catalysed by the highly related DEAD-box proteins p68 and p72
Nucleic Acids Res., May 15, 2001; 29(10): 2088 - 2096.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
E. N. Zaitsev and S. C. Kowalczykowski
A novel pairing process promoted by Escherichia coli RecA protein: inverse DNA and RNA strand exchange
Genes & Dev., March 15, 2000; 14(6): 740 - 749.
[Abstract] [Full Text]


Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
Genome Res. Learn. Mem.
Protein Science RNA Genes Dev.