![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 14, No. 4, pp. 422-434, February 15, 2000
1 Department of Cellular and Molecular Pharmacology,
University of California, San Francisco, San Francisco, California
94143-0450 USA; 2 Department of Biochemistry and Molecular
Biology, Mayo Clinic, Rochester, Minnesota 55905 USA
Multiple molecular chaperones, including Hsp90 and p23, interact
with members of the intracellular receptor (IR) family. To investigate
p23 function, we compared the effects of three p23 proteins on IR
activities, yeast p23 (sba1p) and the two human p23 homologs, p23 and
tsp23. We found that Sba1p was indistinguishable from human p23 in
assays of seven IR activities in both animal cells and in yeast; in
contrast, certain effects of tsp23 were specific to that homolog.
Transcriptional activation by two IRs was increased by expression of
any of the p23 species, whereas activation by five other IRs was
decreased by Sba1p or p23, and unaffected by tsp23. p23 was expressed
in all tissues examined except striated and cardiac muscle, whereas
tsp23 accumulated in a complementary pattern; hence, p23 proteins might
contribute to tissue-specific differences in IR activities. Unlike
Hsp90, which acts on IR aporeceptors to stimulate ligand potency (i.e., hormone-binding affinity), p23 proteins acted on IR holoreceptors to
alter ligand efficiencies (i.e., transcriptional activation activity).
Moreover, the p23 effects developed slowly, requiring prolonged
exposure to hormone. In vitro, p23 interacted preferentially with
hormone-receptor-response element ternary complexes, and stimulated
receptor-DNA dissociation. The dissociation was reversed by addition
of a fragment of the GRIP1 coactivator, suggesting that the two
reactions may be in competition in vivo. Our findings suggest that p23
functions at one or more late steps in IR-mediated signal transduction,
perhaps including receptor recycling and/or reversal of
the response.
[Key Words: intracellular receptor; ligand efficacy; molecular chaperone; p23]
CiteULike 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  N. B. Berry, M. Fan, and K. P. Nephew Estrogen Receptor-{alpha} Hinge-Region Lysines 302 and 303 Regulate Receptor Degradation by the Proteasome Mol. Endocrinol., July 1, 2008; 22(7): 1535 - 1551. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Forafonov, O. A. Toogun, I. Grad, E. Suslova, B. C. Freeman, and D. Picard p23/Sba1p Protects against Hsp90 Inhibitors Independently of Its Intrinsic Chaperone Activity Mol. Cell. Biol., May 15, 2008; 28(10): 3446 - 3456. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. A. Weeks and D. J. Miller The Heat Shock Protein 70 Cochaperone YDJ1 Is Required for Efficient Membrane-Specific Flock House Virus RNA Replication Complex Assembly and Function in Saccharomyces cerevisiae J. Virol., February 15, 2008; 82(4): 2004 - 2012. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. H. Meijsing, C. Elbi, H. F. Luecke, G. L. Hager, and K. R. Yamamoto The Ligand Binding Domain Controls Glucocorticoid Receptor Dynamics Independent of Ligand Release Mol. Cell. Biol., April 1, 2007; 27(7): 2442 - 2451. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Hardcastle, P. Tomlin, C. Norris, J. Richards, M. Cordwell, K. Boxall, M. Rowlands, K. Jones, I. Collins, E. McDonald, et al. A duplexed phenotypic screen for the simultaneous detection of inhibitors of the molecular chaperone heat shock protein 90 and modulators of cellular acetylation Mol. Cancer Ther., March 1, 2007; 6(3): 1112 - 1122. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Fang, D. Ricketson, L. Getubig, and B. Darimont Unliganded and hormone-bound glucocorticoid receptors interact with distinct hydrophobic sites in the Hsp90 C-terminal domain PNAS, December 5, 2006; 103(49): 18487 - 18492. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Grad, T. A. McKee, S. M. Ludwig, G. W. Hoyle, P. Ruiz, W. Wurst, T. Floss, C. A. Miller III, and D. Picard The Hsp90 Cochaperone p23 Is Essential for Perinatal Survival Mol. Cell. Biol., December 1, 2006; 26(23): 8976 - 8983. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Cheung-Flynn, V. Prapapanich, M. B. Cox, D. L. Riggs, C. Suarez-Quian, and D. F. Smith Physiological Role for the Cochaperone FKBP52 in Androgen Receptor Signaling Mol. Endocrinol., June 1, 2005; 19(6): 1654 - 1666. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Wang, R. Thuillier, and M. Culty Prenatal Estrogen Exposure Differentially Affects Estrogen Receptor-Associated Proteins in Rat Testis Gonocytes Biol Reprod, November 1, 2004; 71(5): 1652 - 1664. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. D. Hollingshead, J. R. Petrulis, and G. H. Perdew The Aryl Hydrocarbon (Ah) Receptor Transcriptional Regulator Hepatitis B Virus X-associated Protein 2 Antagonizes p23 Binding to Ah Receptor-Hsp90 Complexes and Is Dispensable for Receptor Function J. Biol. Chem., October 29, 2004; 279(44): 45652 - 45661. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. D. Freedman and K. R. Yamamoto Importin 7 and Importin {alpha}/Importin {beta} Are Nuclear Import Receptors for the Glucocorticoid Receptor Mol. Biol. Cell, May 1, 2004; 15(5): 2276 - 2286. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Botos, W. Xian, D. F. Smith, and C. L. Smith Progesterone Receptor Deficient in Chromatin Binding Has an Altered Cellular State J. Biol. Chem., April 9, 2004; 279(15): 15231 - 15239. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. G. Thackray, D. O. Toft, and S. K. Nordeen Novel Activation Step Required for Transcriptional Competence of Progesterone Receptor on Chromatin Templates Mol. Endocrinol., December 1, 2003; 17(12): 2543 - 2553. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Shatkina, S. Mink, H. Rogatsch, H. Klocker, G. Langer, A. Nestl, and A. C. B. Cato The Cochaperone Bag-1L Enhances Androgen Receptor Action via Interaction with the NH2-Terminal Region of the Receptor Mol. Cell. Biol., October 15, 2003; 23(20): 7189 - 7197. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Warnmark, E. Treuter, A. P. H. Wright, and J.-A. Gustafsson Activation Functions 1 and 2 of Nuclear Receptors: Molecular Strategies for Transcriptional Activation Mol. Endocrinol., October 1, 2003; 17(10): 1901 - 1909. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. F. Walther, C. Lamprecht, A. Ridsdale, I. Groulx, S. Lee, Y. A. Lefebvre, and R. J. G. Hache Nuclear Export of the Glucocorticoid Receptor Is Accelerated by Cell Fusion-dependent Release of Calreticulin J. Biol. Chem., September 26, 2003; 278(39): 37858 - 37864. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Oxelmark, R. Knoblauch, S. Arnal, L. F. Su, M. Schapira, and M. J. Garabedian Genetic Dissection of p23, an Hsp90 Cochaperone, Reveals a Distinct Surface Involved in Estrogen Receptor Signaling J. Biol. Chem., September 19, 2003; 278(38): 36547 - 36555. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. Schmidt, G. M. Wochnik, M. C. Rosenhagen, J. C. Young, F. U. Hartl, F. Holsboer, and T. Rein Essential Role of the Unusual DNA-binding Motif of BAG-1 for Inhibition of the Glucocorticoid Receptor J. Biol. Chem., February 7, 2003; 278(7): 4926 - 4931. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. B. Pratt and D. O. Toft Regulation of Signaling Protein Function and Trafficking by the hsp90/hsp70-Based Chaperone Machinery Experimental Biology and Medicine, February 1, 2003; 228(2): 111 - 133. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. P. Sullivan, B. A. L. Owen, and D. O. Toft The Influence of ATP and p23 on the Conformation of hsp90 J. Biol. Chem., November 22, 2002; 277(48): 45942 - 45948. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. C. Freeman and K. R. Yamamoto Disassembly of Transcriptional Regulatory Complexes by Molecular Chaperones Science, June 21, 2002; 296(5576): 2232 - 2235. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Han, S. Tsui, and T. J. Smith Up-regulation of Prostaglandin E2 Synthesis by Interleukin-1beta in Human Orbital Fibroblasts Involves Coordinate Induction of Prostaglandin-Endoperoxide H Synthase-2 and Glutathione-dependent Prostaglandin E2 Synthase Expression J. Biol. Chem., May 3, 2002; 277(19): 16355 - 16364. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Abbas-Terki, O. Donze, P.-A. Briand, and D. Picard Hsp104 Interacts with Hsp90 Cochaperones in Respiring Yeast Mol. Cell. Biol., November 15, 2001; 21(22): 7569 - 7575. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. S. Mahendroo, K. M. Cala, D. L. Hess, and D. W. Russell Unexpected Virilization in Male Mice Lacking Steroid 5{alpha}-Reductase Enzymes Endocrinology, November 1, 2001; 142(11): 4652 - 4662. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. C. Young, I. Moarefi, and F. U. Hartl Hsp90: a specialized but essential protein-folding tool J. Cell Biol., July 23, 2001; 154(2): 267 - 274. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Chadli, I. Bouhouche, W. Sullivan, B. Stensgard, N. McMahon, M. G. Catelli, and D. O. Toft Dimerization and N-terminal domain proximity underlie the function of the molecular chaperone heat shock protein 90 PNAS, October 23, 2000; (2000) 220430297. [Abstract] [Full Text]
|
|
|
|
 |
|
 R. M. Nissen and K. R. Yamamoto The glucocorticoid receptor inhibits NFkappa B by interfering with serine-2 phosphorylation of the RNA polymerase II carboxy-terminal domain Genes & Dev., September 15, 2000; 14(18): 2314 - 2329. [Abstract] [Full Text]
|
|
|
|
|
|
T. Tanioka, Y. Nakatani, N. Semmyo, M. Murakami, and I. Kudo Molecular Identification of Cytosolic Prostaglandin E2 Synthase That Is Functionally Coupled with Cyclooxygenase-1 in Immediate Prostaglandin E2 Biosynthesis J. Biol. Chem., October 13, 2000; 275(42): 32775 - 32782. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Rao, P. Lee, S. Benzeno, C. Cardozo, J. Albertus, D. M. Robins, and A. J. Caplan Functional Interaction of Human Cdc37 with the Androgen Receptor but Not with the Glucocorticoid Receptor J. Biol. Chem., February 16, 2001; 276(8): 5814 - 5820. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. M. Scholz, K. Cartledge, and N. E. Hall Identification and Characterization of Harc, a Novel Hsp90-associating Relative of Cdc37 J. Biol. Chem., August 10, 2001; 276(33): 30971 - 30979. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Pandit, W. Geissler, G. Harris, and A. Sitlani Allosteric Effects of Dexamethasone and RU486 on Glucocorticoid Receptor-DNA Interactions J. Biol. Chem., January 4, 2002; 277(2): 1538 - 1543. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Chadli, I. Bouhouche, W. Sullivan, B. Stensgard, N. McMahon, M. G. Catelli, and D. O. Toft Dimerization and N-terminal domain proximity underlie the function of the molecular chaperone heat shock protein 90 PNAS, November 7, 2000; 97(23): 12524 - 12529. [Abstract] [Full Text] [PDF]
|
|
