![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 15, No. 1, pp. 30-35, January 1, 2001
Howard Hughes Medical Institute, Programs in Gene Function and
Expression and Molecular Medicine, University of Massachusetts Medical
School, Worcester, Massachusetts 01605, USA
The human 56-kD U2AF65-associated protein (hUAP56), a
member of the DExD/H box protein family of RNA-dependent ATPases, is
required for the stable binding of U2 snRNP to the pre-mRNA
branchpoint. Here we identify a highly conserved Saccharomyces
cerevisiae homolog of hUAP56, yUAP/Sub2p. yUAP/Sub2p can be
functionally substituted for by hUAP56 and, like its human counterpart,
is an essential pre-mRNA splicing factor. yUAP/Sub2p is required for
formation of the commitment complex, the precursor for U2 snRNP
addition. In conjunction with previous studies, we conclude that at
least two DExD/H box proteins, Prp5p and yUAP/Sub2p, mediate the U2 snRNP-branchpoint interaction.
[Key Words: pre-mRNA splicing; hUAp56; SUB2; U2AF; DEAD box protein; U2 snRNP binding; commitment complex]
CiteULike 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  K. P. Kota, S. R. Wagner, E. Huerta, J. M. Underwood, and J. A. Nickerson Binding of ATP to UAP56 is necessary for mRNA export J. Cell Sci., May 1, 2008; 121(9): 1526 - 1537. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. M. S. Elles and O. C. Uhlenbeck Mutation of the arginine finger in the active site of Escherichia coli DbpA abolishes ATPase and helicase activity and confers a dominant slow growth phenotype Nucleic Acids Res., January 17, 2008; 36(1): 41 - 50. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. G. Thakurta, S. P. Selvanathan, A. D. Patterson, G. Gopal, and R. Dhar The Nuclear Export Signal of Splicing Factor Uap56p Interacts with Nuclear Pore-associated Protein Rae1p for mRNA Export in Schizosaccharomyces pombe J. Biol. Chem., June 15, 2007; 282(24): 17507 - 17516. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-I G. Chen, R. E. Moore, H. Y. Ge, M. K. Young, T. D. Lee, and S. W. Stevens Proteomic analysis of in vivo-assembled pre-mRNA splicing complexes expands the catalog of participating factors Nucleic Acids Res., June 9, 2007; 35(12): 3928 - 3944. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Jankowsky and H. Bowers Remodeling of ribonucleoprotein complexes with DExH/D RNA helicases Nucleic Acids Res., September 10, 2006; 34(15): 4181 - 4188. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. J. Preker and C. Guthrie Autoregulation of the mRNA export factor Yra1p requires inefficient splicing of its pre-mRNA RNA, June 1, 2006; 12(6): 994 - 1006. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Granneman, K. A. Bernstein, F. Bleichert, and S. J. Baserga Comprehensive Mutational Analysis of Yeast DEXD/H Box RNA Helicases Required for Small Ribosomal Subunit Synthesis Mol. Cell. Biol., February 15, 2006; 26(4): 1183 - 1194. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. A. Bernstein, S. Granneman, A. V. Lee, S. Manickam, and S. J. Baserga Comprehensive Mutational Analysis of Yeast DEXD/H Box RNA Helicases Involved in Large Ribosomal Subunit Biogenesis Mol. Cell. Biol., February 15, 2006; 26(4): 1195 - 1208. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. J. Brenner and C. Guthrie Genetic Analysis Reveals a Role for the C Terminus of the Saccharomyces cerevisiae GTPase Snu114 During Spliceosome Activation Genetics, July 1, 2005; 170(3): 1063 - 1080. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Shi, O. Cordin, C. M. Minder, P. Linder, and R.-M. Xu Crystal structure of the human ATP-dependent splicing and export factor UAP56 PNAS, December 21, 2004; 101(51): 17628 - 17633. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. CUSTODIO, C. CARVALHO, I. CONDADO, M. ANTONIOU, B. J. BLENCOWE, and M. CARMO-FONSECA In vivo recruitment of exon junction complex proteins to transcription sites in mammalian cell nuclei RNA, April 1, 2004; 10(4): 622 - 633. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Pryor, L. Tung, Z. Yang, F. Kapadia, T.-H. Chang, and L. F. Johnson Growth-regulated expression and G0-specific turnover of the mRNA that encodes URH49, a mammalian DExH/D box protein that is highly related to the mRNA export protein UAP56 Nucleic Acids Res., March 26, 2004; 32(6): 1857 - 1865. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Schneider, E. Campodonico, and B. Schwer Motifs IV and V in the DEAH Box Splicing Factor Prp22 Are Important for RNA Unwinding, and Helicase-defective Prp22 Mutants Are Suppressed by Prp8 J. Biol. Chem., March 5, 2004; 279(10): 8617 - 8626. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Kataoka and G. Dreyfuss A Simple Whole Cell Lysate System for in Vitro Splicing Reveals a Stepwise Assembly of the Exon-Exon Junction Complex J. Biol. Chem., February 20, 2004; 279(8): 7009 - 7013. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Hurt, M.-j. Luo, S. Rother, R. Reed, and K. Strasser Cotranscriptional recruitment of the serine-arginine-rich (SR)-like proteins Gbp2 and Hrb1 to nascent mRNA via the TREX complex PNAS, February 17, 2004; 101(7): 1858 - 1862. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Perriman, I. Barta, G. K. Voeltz, J. Abelson, and M. Ares Jr. ATP requirement for Prp5p function is determined by Cus2p and the structure of U2 small nuclear RNA PNAS, November 25, 2003; 100(24): 13857 - 13862. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. G. Rondon, S. Jimeno, M. Garcia-Rubio, and A. Aguilera Molecular Evidence That the Eukaryotic THO/TREX Complex Is Required for Efficient Transcription Elongation J. Biol. Chem., October 3, 2003; 278(40): 39037 - 39043. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. MACMORRIS, C. BROCKER, and T. BLUMENTHAL UAP56 levels affect viability and mRNA export in Caenorhabditis elegans RNA, July 1, 2003; 9(7): 847 - 857. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. J. Merker and H. L. Klein Role of Transcription in Plasmid Maintenance in the hpr1{Delta} Mutant of Saccharomyces cerevisiae Mol. Cell. Biol., December 15, 2002; 22(24): 8763 - 8773. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Zenklusen, P. Vinciguerra, J.-C. Wyss, and F. Stutz Stable mRNP Formation and Export Require Cotranscriptional Recruitment of the mRNA Export Factors Yra1p and Sub2p by Hpr1p Mol. Cell. Biol., December 1, 2002; 22(23): 8241 - 8253. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Libri, K. Dower, J. Boulay, R. Thomsen, M. Rosbash, and T. H. Jensen Interactions between mRNA Export Commitment, 3'-End Quality Control, and Nuclear Degradation Mol. Cell. Biol., December 1, 2002; 22(23): 8254 - 8266. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. P. Lei and P. A. Silver Intron status and 3'-end formation control cotranscriptional export of mRNA Genes & Dev., November 1, 2002; 16(21): 2761 - 2766. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. L. Reichert, H. Le Hir, M. S. Jurica, and M. J. Moore 5' exon interactions within the human spliceosome establish a framework for exon junction complex structure and assembly Genes & Dev., November 1, 2002; 16(21): 2778 - 2791. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z.-R. Liu p68 RNA Helicase Is an Essential Human Splicing Factor That Acts at the U1 snRNA-5' Splice Site Duplex Mol. Cell. Biol., August 1, 2002; 22(15): 5443 - 5450. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. K. Abu Dayyeh, T. K. Quan, M. Castro, and S. W. Ruby Probing Interactions between the U2 Small Nuclear Ribonucleoprotein and the DEAD-box Protein, Prp5 J. Biol. Chem., June 7, 2002; 277(23): 20221 - 20233. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Libri, F. Duconge, L. Levy, and M. Vinauger A Role for the Psi -U Mismatch in the Recognition of the 5' Splice Site of Yeast Introns by the U1 Small Nuclear Ribonucleoprotein Particle J. Biol. Chem., May 10, 2002; 277(20): 18173 - 18181. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Schneider, H.-R. Hotz, and B. Schwer Characterization of Dominant-negative Mutants of the DEAH-box Splicing Factors Prp22 and Prp16 J. Biol. Chem., May 3, 2002; 277(18): 15452 - 15458. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-Y. Fan, R. J. Merker, and H. L. Klein High-Copy-Number Expression of Sub2p, a Member of the RNA Helicase Superfamily, Suppresses hpr1-Mediated Genomic Instability Mol. Cell. Biol., August 15, 2001; 21(16): 5459 - 5470. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. L. Kistler and C. Guthrie Deletion of MUD2, the yeast homolog of U2AF65, can bypass the requirement for Sub2, an essential spliceosomal ATPase Genes & Dev., January 1, 2001; 15(1): 42 - 49. [Abstract] [Full Text]
|
|
