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Vol. 15, No. 10, pp. 1287-1298, May 15, 2001
Division of Basic Sciences, Fred Hutchinson Cancer Research Center,
Seattle, Washington 98109, USA
A large number of DNA segments are excised from the chromosomes of
the somatic nucleus during development of Tetrahymena
thermophila. How these germline-limited sequences are recognized
and excised is still poorly understood. We have found that many of
these noncoding DNAs are transcribed during nuclear development.
Transcription of the germline-limited M element occurs from both DNA
strands and results in heterogeneous transcripts of < 200 b to > 1 kb. Transcripts are most abundant when developing micro- and
macronuclei begin their differentiation. Transcription is normally
restricted to unrearranged DNA of micronuclei and/or developing nuclei,
but germline-limited DNAs can induce their own transcription when placed into somatic macronuclei. Brief actinomycin D treatment of
conjugating cells blocked M-element excision, providing evidence that
transcription is important for efficient DNA rearrangement. We propose
that transcription targets these germline-limited sequences for
elimination by altering chromatin to ensure their accessibility to the
excision machinery.
[Key Words: DNA rearrangement; intergenic transcription; ciliates; nuclear development]
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