.gif?ad=16248&adview=true)
![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 15, No. 16, pp. 2083-2093, August 15, 2001
Department of Biochemistry and McGill Cancer Center, McGill
University, Montreal, Quebec H3G 1Y6, Canada
Cap-dependent translation is mediated by eIF4F, a protein complex
composed of three subunits as follows: eIF4E, which recognizes the mRNA
5' cap structure; eIF4A, an RNA-helicase; and eIF4G, a scaffolding
protein that binds eIF4E, eIF4A, and the eIF4E-kinase Mnk1
simultaneously. eIF4E is hypophosphorylated and cap-dependent translation is reduced at mitosis. Here, we show that 4E-BP1, a
suppressor of eIF4E function, is also hypophosphorylated in mitosis,
resulting in disruption of the eIF4F complex. Consequently, eIF4E is
sequestered from the eIF4G/Mnk1 complex. These results explain the
specific inhibition of cap-dependent translation in mitosis and also
explain how eIF4E is rendered hypophosphorylated during mitosis.
Furthermore, eIF4E interaction with eIF4GII is strongly decreased
coincident with hyperphosphorylation of eIF4GII. Thus, inhibition of
cap-dependent translation in mitosis results from a combination of
phosphorylation modifications leading to eIF4F complex disruption.
[Key Words: Translation initiation; mitosis; cap-binding complex; internal ribosome entry site]
CiteULike 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  B. Dolniak, E. Katsoulidis, N. Carayol, J. K. Altman, A. J. Redig, M. S. Tallman, T. Ueda, R. Watanabe-Fukunaga, R. Fukunaga, and L. C. Platanias Regulation of Arsenic Trioxide-induced Cellular Responses by Mnk1 and Mnk2 J. Biol. Chem., May 2, 2008; 283(18): 12034 - 12042. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Gendron, J. Charbonneau, D. Dulude, N. Heveker, G. Ferbeyre, and L. Brakier-Gingras The presence of the TAR RNA structure alters the programmed -1 ribosomal frameshift efficiency of the human immunodeficiency virus type 1 (HIV-1) by modifying the rate of translation initiation Nucleic Acids Res., January 17, 2008; 36(1): 30 - 40. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Minshall, M. H. Reiter, D. Weil, and N. Standart CPEB Interacts with an Ovary-specific eIF4E and 4E-T in Early Xenopus Oocytes J. Biol. Chem., December 28, 2007; 282(52): 37389 - 37401. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. C. Vary and C. J. Lynch Nutrient Signaling Components Controlling Protein Synthesis in Striated Muscle J. Nutr., August 1, 2007; 137(8): 1835 - 1843. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. F. Monzingo, S. Dhaliwal, A. Dutt-Chaudhuri, A. Lyon, J. H. Sadow, D. W. Hoffman, J. D. Robertus, and K. S. Browning The Structure of Eukaryotic Translation Initiation Factor-4E from Wheat Reveals a Novel Disulfide Bond Plant Physiology, April 1, 2007; 143(4): 1504 - 1518. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. J. Coldwell and S. J. Morley Specific Isoforms of Translation Initiation Factor 4GI Show Differences in Translational Activity Mol. Cell. Biol., November 15, 2006; 26(22): 8448 - 8460. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. D. Baird, M. Turcotte, R. G. Korneluk, and M. Holcik Searching for IRES RNA, October 1, 2006; 12(10): 1755 - 1785. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. C. Vary and C. J. Lynch Meal feeding enhances formation of eIF4F in skeletal muscle: role of increased eIF4E availability and eIF4G phosphorylation Am J Physiol Endocrinol Metab, April 1, 2006; 290(4): E631 - E642. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Jin, S.-K. Hwang, K. Yu, H. K. Anderson, Y.-S. Lee, K. H. Lee, A.-C. Prats, D. Morello, G. R. Beck Jr., and M.-H. Cho A High Inorganic Phosphate Diet Perturbs Brain Growth, Alters Akt-ERK Signaling, and Results in Changes in Cap-Dependent Translation Toxicol. Sci., March 1, 2006; 90(1): 221 - 229. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. C. Vary IGF-I stimulates protein synthesis in skeletal muscle through multiple signaling pathways during sepsis Am J Physiol Regulatory Integrative Comp Physiol, February 1, 2006; 290(2): R313 - R321. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. A. Ferraiuolo, S. Basak, J. Dostie, E. L. Murray, D. R. Schoenberg, and N. Sonenberg A role for the eIF4E-binding protein 4E-T in P-body formation and mRNA decay J. Cell Biol., September 12, 2005; 170(6): 913 - 924. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Drea, D. J. Leader, B. C. Arnold, P. Shaw, L. Dolan, and J. H. Doonan Systematic Spatial Analysis of Gene Expression during Wheat Caryopsis Development PLANT CELL, August 1, 2005; 17(8): 2172 - 2185. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Gorrini, F. Loreni, V. Gandin, L. A. Sala, N. Sonenberg, P. C. Marchisio, and S. Biffo Fibronectin controls cap-dependent translation through {beta}1 integrin and eukaryotic initiation factors 4 and 2 coordinated pathways PNAS, June 28, 2005; 102(26): 9200 - 9205. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Lahlou, M. Fanjul, L. Pradayrol, C. Susini, and S. Pyronnet Restoration of Functional Gap Junctions through Internal Ribosome Entry Site-Dependent Synthesis of Endogenous Connexins in Density-Inhibited Cancer Cells Mol. Cell. Biol., May 15, 2005; 25(10): 4034 - 4045. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Cinar, A. De Benedetti, and M. R. Freeman Post-Transcriptional Regulation of the Androgen Receptor by Mammalian Target of Rapamycin Cancer Res., April 1, 2005; 65(7): 2547 - 2553. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Shi, A. Sharma, H. Wu, A. Lichtenstein, and J. Gera Cyclin D1 and c-myc Internal Ribosome Entry Site (IRES)-dependent Translation Is Regulated by AKT Activity and Enhanced by Rapamycin through a p38 MAPK- and ERK-dependent Pathway J. Biol. Chem., March 25, 2005; 280(12): 10964 - 10973. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Seal, R. Temperley, J. Wilusz, R. N. Lightowlers, and Z. M. A. Chrzanowska-Lightowlers Serum-deprivation stimulates cap-binding by PARN at the expense of eIF4E, consistent with the observed decrease in mRNA stability Nucleic Acids Res., January 14, 2005; 33(1): 376 - 387. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Caron, M. Charon, E. Cramer, N. Sonenberg, and I. Dusanter-Fourt Selective Modification of Eukaryotic Initiation Factor 4F (eIF4F) at the Onset of Cell Differentiation: Recruitment of eIF4GII and Long-Lasting Phosphorylation of eIF4E Mol. Cell. Biol., June 1, 2004; 24(11): 4920 - 4928. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Qin and P. Sarnow Preferential Translation of Internal Ribosome Entry Site-containing mRNAs during the Mitotic Cycle in Mammalian Cells J. Biol. Chem., April 2, 2004; 279(14): 13721 - 13728. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Walsh and I. Mohr Phosphorylation of eIF4E by Mnk-1 enhances HSV-1 translation and replication in quiescent cells Genes & Dev., March 15, 2004; 18(6): 660 - 672. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. L. Wayne, W. Lee, S. Michel, J. Dyer, and W. S. Sossin Activity-Dependent Regulation of Neurohormone Synthesis and Its Impact on Reproductive Behavior in Aplysia Biol Reprod, February 1, 2004; 70(2): 277 - 281. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. E. Wilhelm, M. Hilton, Q. Amos, and W. J. Henzel Cup is an eIF4E binding protein required for both the translational repression of oskar and the recruitment of Barentsz J. Cell Biol., December 22, 2003; 163(6): 1197 - 1204. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Qin, B. Raught, N. Sonenberg, E. G. Goldstein, and A. M. Edelman Phosphorylation Screening Identifies Translational Initiation Factor 4GII as an Intracellular Target of Ca2+/Calmodulin-dependent Protein Kinase I J. Biol. Chem., December 5, 2003; 278(49): 48570 - 48579. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Brasey, M. Lopez-Lastra, T. Ohlmann, N. Beerens, B. Berkhout, J.-L. Darlix, and N. Sonenberg The Leader of Human Immunodeficiency Virus Type 1 Genomic RNA Harbors an Internal Ribosome Entry Segment That Is Active during the G2/M Phase of the Cell Cycle J. Virol., April 1, 2003; 77(7): 3939 - 3949. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Boussadia, M. Niepmann, L. Creancier, A.-C. Prats, F. Dautry, and H. Jacquemin-Sablon Unr Is Required In Vivo for Efficient Initiation of Translation from the Internal Ribosome Entry Sites of both Rhinovirus and Poliovirus J. Virol., March 15, 2003; 77(6): 3353 - 3359. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. H. Kim, K. Y. Paek, K. Choi, T.-D. Kim, B. Hahm, K.-T. Kim, and S. K. Jang Heterogeneous Nuclear Ribonucleoprotein C Modulates Translation of c-myc mRNA in a Cell Cycle Phase-Dependent Manner Mol. Cell. Biol., January 15, 2003; 23(2): 708 - 720. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Niessing, S. Blanke, and H. Jackle Bicoid associates with the 5'-cap-bound complex of caudal mRNA and represses translation Genes & Dev., October 1, 2002; 16(19): 2576 - 2582. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. J. Wolgemuth, E. Laurion, and K. M. Lele Regulation of the Mitotic and Meiotic Cell Cycles in the Male Germ Line Recent Prog. Horm. Res., January 1, 2002; 57(1): 75 - 101. [Abstract] [Full Text] [PDF]
|
|
