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Vol. 15, No. 18, pp. 2408-2420, September 15, 2001
Department of Genetics, Washington University School of Medicine,
St. Louis, Missouri 63110, USA
Caenorhabditis elegans GLD-1, a KH motif containing
RNA-binding protein of the GSG/STAR subfamily, controls diverse aspects of germ line development, suggesting that it may have multiple mRNA
targets. We used an immunoprecipitation/subtractive
hybridization/cloning strategy to identify 15 mRNAs that are putative
targets of GLD-1 binding and regulation. For one target, the
rme-2 yolk receptor mRNA, GLD-1 acts as a translational
repressor to spatially restrict RME-2 accumulation, and thus yolk
uptake, to late-stage oocytes. We found that GLD-1 binds sequences in
both 5' coding and the 3' untranslated region of rme-2 mRNA.
Initial characterization of the other 14 targets shows that (1) they
are coexpressed with GLD-1; (2) they can have mutant/RNA-mediated
interference depletion phenotypes indicating functions in germ line
development or as maternal products necessary for early embryogenesis;
and (3) GLD-1 may coregulate mRNAs corresponding to functionally
redundant subsets of genes within two gene families. Thus, a diverse
set of genes have come under GLD-1-mediated regulation to achieve
normal germ line development. Previous work identified tra-2 as
a GLD-1 target for germ line sex determination. Comparisons of
GLD-1-mediated translational control of rme-2 and tra-2
suggests that the mechanisms may differ for distinct target mRNA
species.
[Key Words: Translational control; target mRNAs; maxi-KH; Caenorhabditis elegans; yolk receptor; germ line]
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