![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 15, No. 23, pp. 3104-3117, December 1, 2001
Department of 1 Biochemistry and the
2 McGill Cancer Centre, McGill University, Montreal, Quebec
H3G 1Y6, Canada; 3 Stowers Institute for Medical Research,
Kansas City, Missouri 64110, USA; 4 Department of Biochemistry
and Molecular Biology, University of Oklahoma Health Sciences Center,
Oklahoma City, Oklahoma 73190, USA; 5 Department of Molecular
and Cellular Biology, Medical Institute of Bioregulation, Kyushu
University, Kawaguchi, Saitama 332-012, Japan; 6 Dana-Farber
Cancer Institute and Harvard Medical School, Boston, Massachusetts
02115, USA; and 7 Department of Biochemistry and Molecular Biology,
University of Kansas Medical Center, Kansas City, Kansas 66160, USA
Although MDM2 plays a major role in regulating the stability of the
p53 tumor suppressor protein, other poorly understood MDM2-independent
pathways also exist. Human adenoviruses have evolved strategies to
regulate p53 function and stability to permit efficient viral
replication. One mechanism involves adenovirus E1B55K and E4orf6
proteins, which collaborate to target p53 for degradation. To determine
the mechanism of this process, a multiprotein E4orf6-associated complex
was purified and shown to contain a novel Cullin-containing E3
ubiquitin ligase that is (1) composed of Cullin family member Cul5,
Elongins B and C, and the RING-H2 finger protein Rbx1(ROC1); (2)
remarkably similar to the von Hippel-Lindau tumor suppressor and SCF
(Skp1-Cul1/Cdc53-F-box) E3
ubiquitin ligase complexes; and (3) capable of stimulating
ubiquitination of p53 in vitro in the presence of E1/E2
ubiquitin-activating and -conjugating enzymes. Cullins are activated by
NEDD8 modification; therefore, to determine whether Cullin complexes
are required for adenovirus-induced p53 degradation, studies were
conducted in ts41 Chinese hamster ovary cells that are temperature
sensitive for the NEDD8 pathway. E4orf6/E1B55K failed to induce the
degradation of p53 at the nonpermissive temperature. Thus, our results
identify a novel role for the Cullin-based machinery in regulation of p53.
[Key Words: p53; adenovirus; protein degradation; Cullin complexes; ubiquitination; Elongin B; Elongin C; NEDD8]
CiteULike 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  L. J. Marshall, A. C. Moore, M. Ohki, I. Kitabayashi, D. Patterson, and D. A. Ornelles RUNX1 Permits E4orf6-Directed Nuclear Localization of the Adenovirus E1B-55K Protein and Associates with Centers of Viral DNA and RNA Synthesis J. Virol., July 1, 2008; 82(13): 6395 - 6408. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Zhao, R. J. Madden-Fuentes, B. X. Lou, J. M. Pipas, J. Gerhardt, C. J. Rigell, and E. Fanning Ataxia Telangiectasia-Mutated Damage-Signaling Kinase- and Proteasome-Dependent Destruction of Mre11-Rad50-Nbs1 Subunits in Simian Virus 40-Infected Primate Cells J. Virol., June 1, 2008; 82(11): 5316 - 5328. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Nayak, K. D. Farris, and D. J. Pintel E4Orf6-E1B-55k-Dependent Degradation of De Novo-Generated Adeno-Associated Virus Type 5 Rep52 and Capsid Proteins Employs a Cullin 5-Containing E3 Ligase Complex J. Virol., April 1, 2008; 82(7): 3803 - 3808. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Zheng, X.-M. Rao, J. G. Gomez-Gutierrez, H. Hao, K. M. McMasters, and H. S. Zhou Adenovirus E1B55K Region Is Required To Enhance Cyclin E Expression for Efficient Viral DNA Replication J. Virol., April 1, 2008; 82(7): 3415 - 3427. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Blanchette, K. Kindsmuller, P. Groitl, F. Dallaire, T. Speiseder, P. E. Branton, and T. Dobner Control of mRNA Export by Adenovirus E4orf6 and E1B55K Proteins during Productive Infection Requires E4orf6 Ubiquitin Ligase Activity J. Virol., March 15, 2008; 82(6): 2642 - 2651. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. R. Gallegos, J. Litersky, H. Lee, Y. Sun, K. Nakayama, K. Nakayama, and H. Lu SCF TrCP1 Activates and Ubiquitylates TAp63{gamma} J. Biol. Chem., January 4, 2008; 283(1): 66 - 75. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. A. Schwartz, J. A. Palacios, G. D. Cassell, S. Adam, M. Giacca, and M. D. Weitzman The Mre11/Rad50/Nbs1 Complex Limits Adeno-Associated Virus Transduction and Replication J. Virol., December 1, 2007; 81(23): 12936 - 12945. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Huh, X. Zhou, H. Hayakawa, J.-Y. Cho, T. A. Libermann, J. Jin, J. Wade Harper, and K. Munger Human Papillomavirus Type 16 E7 Oncoprotein Associates with the Cullin 2 Ubiquitin Ligase Complex, Which Contributes to Degradation of the Retinoblastoma Tumor Suppressor J. Virol., September 15, 2007; 81(18): 9737 - 9747. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Baker, K. J. Rohleder, L. A. Hanakahi, and G. Ketner Adenovirus E4 34k and E1b 55k Oncoproteins Target Host DNA Ligase IV for Proteasomal Degradation J. Virol., July 1, 2007; 81(13): 7034 - 7040. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Luo, E. Ehrlich, Z. Xiao, W. Zhang, G. Ketner, and X.-F. Yu Adenovirus E4orf6 assembles with Cullin5-ElonginB-ElonginC E3 ubiquitin ligase through an HIV/SIV Vif-like BC-box to regulate p53 FASEB J, June 1, 2007; 21(8): 1742 - 1750. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Boggio, A. Passafaro, and S. Chiocca Targeting SUMO E1 to Ubiquitin Ligases: A VIRAL STRATEGY TO COUNTERACT SUMOYLATION J. Biol. Chem., May 25, 2007; 282(21): 15376 - 15382. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. J. Ullman, N. C. Reich, and P. Hearing Adenovirus E4 ORF3 Protein Inhibits the Interferon-Mediated Antiviral Response J. Virol., May 1, 2007; 81(9): 4744 - 4752. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Kindsmuller, P. Groitl, B. Hartl, P. Blanchette, J. Hauber, and T. Dobner Intranuclear targeting and nuclear export of the adenovirus E1B-55K protein are regulated by SUMO1 conjugation PNAS, April 17, 2007; 104(16): 6684 - 6689. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. S. Hart, D. Ornelles, and C. Koumenis The Adenoviral E4orf6 Protein Induces Atypical Apoptosis in Response to DNA Damage J. Biol. Chem., March 2, 2007; 282(9): 6061 - 6067. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Nayak and D. J. Pintel Positive and Negative Effects of Adenovirus Type 5 Helper Functions on Adeno-Associated Virus Type 5 (AAV5) Protein Accumulation Govern AAV5 Virus Production J. Virol., March 1, 2007; 81(5): 2205 - 2212. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. L. Woo and A. J. Berk Adenovirus Ubiquitin-Protein Ligase Stimulates Viral Late mRNA Nuclear Export J. Virol., January 15, 2007; 81(2): 575 - 587. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Xiao, E. Ehrlich, K. Luo, Y. Xiong, and X.-F. Yu Zinc chelation inhibits HIV Vif activity and liberates antiviral function of the cytidine deaminase APOBEC3G FASEB J, January 1, 2007; 21(1): 217 - 222. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Paul, J. Cui, and E. L. Maynard Zinc binding to the HCCH motif of HIV-1 virion infectivity factor induces a conformational change that mediates protein-protein interactions PNAS, December 5, 2006; 103(49): 18475 - 18480. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Oh, E.-W. Lee, Y. H. Sung, M.-R. Yang, J. Ghim, H.-W. Lee, and J. Song Jab1 Induces the Cytoplasmic Localization and Degradation of p53 in Coordination with Hdm2 J. Biol. Chem., June 23, 2006; 281(25): 17457 - 17465. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Mehle, E. R. Thomas, K. S. Rajendran, and D. Gabuzda A Zinc-binding Region in Vif Binds Cul5 and Determines Cullin Selection J. Biol. Chem., June 23, 2006; 281(25): 17259 - 17265. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Barry and K. Fruh Viral Modulators of Cullin RING Ubiquitin Ligases: Culling the Host Defense Sci. Signal., May 16, 2006; 2006(335): pe21 - pe21. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Subramanian, S. Vijayalingam, and G. Chinnadurai Genetic Identification of Adenovirus Type 5 Genes That Influence Viral Spread J. Virol., February 15, 2006; 80(4): 2000 - 2012. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Pazhouhandeh, M. Dieterle, K. Marrocco, E. Lechner, B. Berry, Vér. Brault, O. Hemmer, T. Kretsch, K. E. Richards, P. Genschik, et al. From the Cover: F-box-like domain in the polerovirus protein P0 is required for silencing suppressor function PNAS, February 7, 2006; 103(6): 1994 - 1999. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Liu, A. Shevchenko, A. Shevchenko, and A. J. Berk Adenovirus Exploits the Cellular Aggresome Response To Accelerate Inactivation of the MRN Complex J. Virol., November 15, 2005; 79(22): 14004 - 14016. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Shirata, A. Kudoh, T. Daikoku, Y. Tatsumi, M. Fujita, T. Kiyono, Y. Sugaya, H. Isomura, K. Ishizaki, and T. Tsurumi Activation of Ataxia Telangiectasia-mutated DNA Damage Checkpoint Signal Transduction Elicited by Herpes Simplex Virus Infection J. Biol. Chem., August 26, 2005; 280(34): 30336 - 30341. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. B. Johnston, G. Wang, J. W. Barrett, S. H. Nazarian, K. Colwill, M. Moran, and G. McFadden Myxoma Virus M-T5 Protects Infected Cells from the Stress of Cell Cycle Arrest through Its Interaction with Host Cell Cullin-1 J. Virol., August 15, 2005; 79(16): 10750 - 10763. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. H. Stracker, D. V. Lee, C. T. Carson, F. D. Araujo, D. A. Ornelles, and M. D. Weitzman Serotype-Specific Reorganization of the Mre11 Complex by Adenoviral E4orf3 Proteins J. Virol., June 1, 2005; 79(11): 6664 - 6673. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. D. Evans and P. Hearing Relocalization of the Mre11-Rad50-Nbs1 Complex by the Adenovirus E4 ORF3 Protein Is Required for Viral Replication J. Virol., May 15, 2005; 79(10): 6207 - 6215. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Kobayashi, A. Takaori-Kondo, Y. Miyauchi, K. Iwai, and T. Uchiyama Ubiquitination of APOBEC3G by an HIV-1 Vif-Cullin5-Elongin B-Elongin C Complex Is Essential for Vif Function J. Biol. Chem., May 13, 2005; 280(19): 18573 - 18578. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Kudoh, M. Fujita, L. Zhang, N. Shirata, T. Daikoku, Y. Sugaya, H. Isomura, Y. Nishiyama, and T. Tsurumi Epstein-Barr Virus Lytic Replication Elicits ATM Checkpoint Signal Transduction While Providing an S-phase-like Cellular Environment J. Biol. Chem., March 4, 2005; 280(9): 8156 - 8163. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. S. Knight, N. Sharma, and E. S. Robertson SCFSkp2 Complex Targeted by Epstein-Barr Virus Essential Nuclear Antigen Mol. Cell. Biol., March 1, 2005; 25(5): 1749 - 1763. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. L. Heuze, F. C. Guibal, C. A. Banks, J. W. Conaway, R. C. Conaway, Y. E. Cayre, A. Benecke, and P. G. Lutz ASB2 Is an Elongin BC-interacting Protein That Can Assemble with Cullin 5 and Rbx1 to Reconstitute an E3 Ubiquitin Ligase Complex J. Biol. Chem., February 18, 2005; 280(7): 5468 - 5474. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. S. Hart, S. M. Yannone, C. Naczki, J. S. Orlando, S. B. Waters, S. A. Akman, D. J. Chen, D. Ornelles, and C. Koumenis The Adenovirus E4orf6 Protein Inhibits DNA Double Strand Break Repair and Radiosensitizes Human Tumor Cells in an E1B-55K-independent Manner J. Biol. Chem., January 14, 2005; 280(2): 1474 - 1481. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Weber, A. Bernhardt, M. Dieterle, P. Hano, A. Mutlu, M. Estelle, P. Genschik, and H. Hellmann Arabidopsis AtCUL3a and AtCUL3b Form Complexes with Members of the BTB/POZ-MATH Protein Family Plant Physiology, January 1, 2005; 137(1): 83 - 93. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Mehle, J. Goncalves, M. Santa-Marta, M. McPike, and D. Gabuzda Phosphorylation of a novel SOCS-box regulates assembly of the HIV-1 Vif-Cul5 complex that promotes APOBEC3G degradation Genes & Dev., December 1, 2004; 18(23): 2861 - 2866. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Blanchette, C. Y. Cheng, Q. Yan, G. Ketner, D. A. Ornelles, T. Dobner, R. C. Conaway, J. W. Conaway, and P. E. Branton Both BC-Box Motifs of Adenovirus Protein E4orf6 Are Required To Efficiently Assemble an E3 Ligase Complex That Degrades p53 Mol. Cell. Biol., November 1, 2004; 24(21): 9619 - 9629. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. N. Shepard and D. A. Ornelles Diverse Roles for E4orf3 at Late Times of Infection Revealed in an E1B 55-Kilodalton Protein Mutant Background J. Virol., September 15, 2004; 78(18): 9924 - 9935. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Nishitani, Z. Lygerou, and T. Nishimoto Proteolysis of DNA Replication Licensing Factor Cdt1 in S-phase Is Performed Independently of Geminin through Its N-terminal Region J. Biol. Chem., July 16, 2004; 279(29): 30807 - 30816. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. Hobom and M. Dobbelstein E1B-55-Kilodalton Protein Is Not Required To Block p53-Induced Transcription during Adenovirus Infection J. Virol., July 15, 2004; 78(14): 7685 - 7697. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Maemondo, Y. Saijo, K. Narumi, T. Kikuchi, K. Usui, R. Tazawa, K. Matsumoto, T. Nakamura, K. Sasaki, M. Takahashi, et al. Gene Therapy with Secretory Leukoprotease Inhibitor Promoter-Controlled Replication-Competent Adenovirus for Non-Small Cell Lung Cancer Cancer Res., July 1, 2004; 64(13): 4611 - 4620. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Shackelford and J. S. Pagano Tumor Viruses and Cell Signaling Pathways: Deubiquitination versus Ubiquitination Mol. Cell. Biol., June 15, 2004; 24(12): 5089 - 5093. [Full Text] [PDF]
|
|
|
|
 |
|
 F. Bernassola, P. Salomoni, A. Oberst, C. J. Di Como, M. Pagano, G. Melino, and P. P. Pandolfi Ubiquitin-dependent Degradation of p73 Is Inhibited by PML J. Exp. Med., June 7, 2004; 199(11): 1545 - 1557. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. S. Mohammadi, E. A. Ketner, D. C. Johns, and G. Ketner Expression of the adenovirus E4 34k oncoprotein inhibits repair of double strand breaks in the cellular genome of a 293-based inducible cell line Nucleic Acids Res., May 11, 2004; 32(8): 2652 - 2659. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. R. Kahl and A. R. Means Calcineurin Regulates Cyclin D1 Accumulation in Growth-stimulated Fibroblasts Mol. Biol. Cell, April 1, 2004; 15(4): 1833 - 1842. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. H. Ali, J. S. Kasper, T. Arai, and J. A. DeCaprio Cul7/p185/p193 Binding to Simian Virus 40 Large T Antigen Has a Role in Cellular Transformation J. Virol., March 15, 2004; 78(6): 2749 - 2757. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Y. Zhao and D. Liao Sequestration of p53 in the Cytoplasm by Adenovirus Type 12 E1B 55-Kilodalton Oncoprotein Is Required for Inhibition of p53-Mediated Apoptosis J. Virol., December 15, 2003; 77(24): 13171 - 13181. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Cuconati, C. Mukherjee, D. Perez, and E. White DNA damage response and MCL-1 destruction initiate apoptosis in adenovirus-infected cells Genes & Dev., December 1, 2003; 17(23): 2922 - 2932. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Van Dort, P. Zhao, K. Parmelee, B. Capps, A. Poel, L. Listenberger, J. Kossoris, B. Wasilevich, D. Murrey, P. Clare, et al. VACM-1, a cul-5 gene, inhibits cellular growth by a mechanism that involves MAPK and p53 signaling pathways Am J Physiol Cell Physiol, December 1, 2003; 285(6): C1386 - C1396. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Yu, Y. Yu, B. Liu, K. Luo, W. Kong, P. Mao, and X.-F. Yu Induction of APOBEC3G Ubiquitination and Degradation by an HIV-1 Vif-Cul5-SCF Complex Science, November 7, 2003; 302(5647): 1056 - 1060. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Y. Zhao, A. L. Colosimo, Y. Liu, Y. Wan, and D. Liao Adenovirus E1B 55-Kilodalton Oncoprotein Binds to Daxx and Eliminates Enhancement of p53-Dependent Transcription by Daxx J. Virol., November 1, 2003; 77(21): 11809 - 11821. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Zhao, F. Granberg, L. Elfineh, U. Pettersson, and C. Svensson Strategic Attack on Host Cell Gene Expression during Adenovirus Infection J. Virol., October 15, 2003; 77(20): 11006 - 11015. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Boutell and R. D. Everett The Herpes Simplex Virus Type 1 (HSV-1) Regulatory Protein ICP0 Interacts with and Ubiquitinates p53 J. Biol. Chem., September 19, 2003; 278(38): 36596 - 36602. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Chastain-Moore, T. Roberts, D. A. Trott, R. F. Newbold, and D. A. Ornelles An Activity Associated with Human Chromosome 21 Permits Nuclear Colocalization of the Adenovirus E1B-55K and E4orf6 Proteins and Promotes Viral Late Gene Expression J. Virol., July 15, 2003; 77(14): 8087 - 8098. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Zhao, X.-M. Rao, X. Xie, L. Li, T. C. Thompson, K. M. McMasters, and H. S. Zhou Adenovirus with Insertion-mutated E1A Selectively Propagates in Liver Cancer Cells and Destroys Tumors in Vivo Cancer Res., June 15, 2003; 63(12): 3073 - 3078. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Lotem, H. Gal, R. Kama, N. Amariglio, G. Rechavi, E. Domany, L. Sachs, and D. Givol Inhibition of p53-induced apoptosis without affecting expression of p53-regulated genes PNAS, May 27, 2003; 100(11): 6718 - 6723. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. D. Evans and P. Hearing Distinct Roles of the Adenovirus E4 ORF3 Protein in Viral DNA Replication and Inhibition of Genome Concatenation J. Virol., May 1, 2003; 77(9): 5295 - 5304. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Serino, H. Su, Z. Peng, T. Tsuge, N. Wei, H. Gu, and X. W. Deng Characterization of the Last Subunit of the Arabidopsis COP9 Signalosome: Implications for the Overall Structure and Origin of the Complex PLANT CELL, March 1, 2003; 15(3): 719 - 731. |
