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Vol. 16, No. 1, pp. 22-26, January 1, 2002
induces adipogenesis through PPAR
: a unified pathway
1 Dana-Farber Cancer Institute, Boston, Massachusetts
02115, USA; 2 Lipid Unit, Massachusetts General Hospital,
Boston, Massachusetts 02114, USA; 3 Laboratory of Metabolism,
National Cancer Institute, Bethesda, Maryland 20892, USA;
4 Department of Cell Biology, Harvard Medical School, Boston,
Massachusetts 02115, USA
PPAR
and C/EBP
are critical transcription factors in
adipogenesis, but the precise role of these proteins has been difficult to ascertain because they positively regulate each other's expression. Questions remain about whether these factors operate independently in
separate, parallel pathways of differentiation, or whether a single
pathway exists. PPAR can promote adipogenesis in C/EBP-deficient cells, but the converse has not been tested. We have created an immortalized line of fibroblasts lacking PPAR, which we use to show
that C/EBP has no ability to promote adipogenesis in the absence of
PPAR. These results indicate that C/EBP and PPAR participate
in a single pathway of fat cell development with PPAR being the
proximal effector of adipogenesis.
[Key Words:
Adipogenesis; PPAR; C/EBP; PPAR
null fibroblasts]
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