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Vol. 16, No. 1, pp. 72-84, January 1, 2002

RESEARCH PAPER
The KH-type RNA-binding protein PSI is required for Drosophila viability, male fertility, and cellular mRNA processing

Emmanuel Labourier, Marco Blanchette, Jennie W. Feiger,1 Melissa D. Adams,2 and Donald C. Rio3

Department of Molecular and Cell Biology, University of California, Berkeley, California 94720, USA

Direct interactions between RNA-binding proteins and snRNP particles modulate eukaryotic pre-mRNA processing patterns to control gene expression. Here, we report that the conserved U1 snRNP-interacting RNA-binding protein PSI is essential for Drosophila viability. A null PSI mutation is recessive lethal at the first-instar larval stage, and lethality is fully rescued by transgenes expressing the PSI protein. A mutant transgene that lacks the PSI-U1 snRNP-interaction domain restores viability but shows courtship behavior abnormalities and meiosis defects during spermatogenesis, resulting in a complete male sterility phenotype. Using cDNA microarrays, we have identified specific target mRNAs with altered expression profiles in these mutant males. A subset of these transcripts is also found associated with PSI in endogenous immunopurified ribonucleoprotein complexes. One specific target, the hrp40/squid transcript, shows an altered pre-mRNA splicing pattern in PSI mutant testes. We conclude that a functional association between the PSI protein and the spliceosomal U1 snRNP particle is required for normal Drosophila development and for the processing of specific PSI-interacting cellular transcripts. These results also validate the use of cDNA microarrays to characterize in vivo RNA-processing defects and alternative pre-mRNA splicing patterns.

[Key Words: Pre-mRNA splicing; U1 snRNP 70K; microarray; spermatogenesis; Drosophila]

Present addresses: 1GenRISK Program, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA; 2Department of Biology, University of North Carolina, Chapel Hill, NC 27599, USA.

3 Corresponding author.

GENES & DEVELOPMENT 16:72-84 © 2002 by Cold Spring Harbor Laboratory Press  ISSN 0890-9369/02 $5.00
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