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Vol. 16, No. 12, pp. 1498-1508, June 15, 2002
1 Division of Basic Sciences, Fred Hutchinson Cancer
Research Center, Seattle, Washington 98109, USA;
2 Molecular and Cellular Biology Program, University of
Washington, Seattle, Washington 98195, USA
Previous studies of mitosis show that capture of single kinetochores
by microtubules from both centrosomes (merotelic orientation) is a
major cause of aneuploidy. We have characterized hcp-6, a temperature-sensitive chromosome segregation mutant in C. elegans that exhibits chromosomes attached to both poles via a
single sister kinetochore. We demonstrate that the primary defect in this mutant is a failure to fully condense chromosomes during prophase.
Although centromere formation and sister centromere resolution remain
unaffected in hcp-6, the chromosomes lack the rigidity of
wild-type chromosomes and twist around the long axis of the chromosome.
As such, they are unable to establish a proper orientation at
prometaphase, allowing individual kinetochores to be captured by
microtubules from both poles. We therefore propose that chromosome
rigidity plays an essential role in maintaining chromosome orientation
to prevent merotelic capture.
[Key Words: C. elegans; centromere; kinetochore; chromosome condensation; bipolar attachment; aneuploidy]
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