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Vol. 16, No. 13, pp. 1627-1639, July 1, 2002
1 Institute of Biochemistry, Federal Institute of
Technology, CH-8092 Zürich, Switzerland; 2 Department of
Molecular, Cellular and Developmental Biology, Yale University,
New Haven, Connecticut 06520, USA
Cell division is the result of two major cytoskeletal events:
partition of the chromatids by the mitotic spindle and cleavage of the
cell by the cytokinetic apparatus. Spatial coordination of these events
ensures that each daughter cell inherits a nucleus. Here we show that,
in budding yeast, capture and shrinkage of astral microtubules at the
bud neck is required to position the spindle relative to the cleavage
apparatus. Capture required the septins and the microtubule-associated
protein Kar9. Like Kar9-defective cells, cells lacking the septin ring
failed to position their spindle correctly and showed an increased
frequency of nuclear missegregation. Microtubule attachment at the bud
neck was followed by shrinkage and a pulling action on the spindle.
Enhancement of microtubule shrinkage at the bud neck required the
Par-1-related, septin-dependent kinases (SDK) Hsl1 and Gin4. Neither
the formin Bnr1 nor the actomyosin contractile ring was required for
either microtubule capture or microtubule shrinkage. Together, our
results indicate that septins and septin-dependent kinases may
coordinate microtubule and actin functions in cell division.
[Key Words: Cytokinesis; septin; microtubule; spindle positioning; cell cortex]
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