.gif?ad=16839&adview=true)
![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 16, No. 13, pp. 1696-1706, July 1, 2002
Department of Biochemistry and Molecular Biology, University of
Southern Denmark, Campusvej 55, DK-5230 Odense M, Denmark
The physiological role of Escherichia coli Spot 42 RNA has
remained obscure, even though the 109-nucleotide RNA was discovered almost three decades ago. Structural features of Spot 42 RNA and previous work suggested to us that the RNA might be a regulator of
discoordinate gene expression of the galactose operon, a control that
is only understood at the phenomenological level. The effects of
controlled expression of Spot 42 RNA or deleting the gene (spf) encoding the RNA supported this hypothesis. Down-regulation of galK expression, the third gene in the gal operon, was
only observed in the presence of Spot 42 RNA and required growth
conditions that caused derepression of the spf gene. Subsequent
biochemical studies showed that Spot 42 RNA specifically bound at the
galK Shine-Dalgarno region of the galETKM mRNA, thereby
blocking ribosome binding. We conclude that Spot 42 RNA is an antisense
RNA that acts to differentially regulate genes that are expressed from the same transcription unit. Our results reveal an interesting mechanism by which the expression of a promoter distal gene in an
operon can be modulated and underline the importance of antisense control in bacterial gene regulation.
[Key Words: Antisense RNA; galactose operon; riboregulation; small RNAs; Spot 42 RNA; translational regulation]
CiteULike 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  B. Tjaden TargetRNA: a tool for predicting targets of small RNA action in bacteria Nucleic Acids Res., July 1, 2008; 36(suppl_2): W109 - W113. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. S. Nielsen, A. Boggild, C. B.F. Andersen, G. Nielsen, A. Boysen, D. E. Brodersen, and P. Valentin-Hansen An Hfq-like protein in archaea: Crystal structure and functional characterization of the Sm protein from Methanococcus jannaschii RNA, December 1, 2007; 13(12): 2213 - 2223. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Heidrich, I. Moll, and S. Brantl In vitro analysis of the interaction between the small RNA SR1 and its primary target ahrC mRNA Nucleic Acids Res., July 26, 2007; 35(13): 4331 - 4346. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. K. Vanderpool and S. Gottesman The Novel Transcription Factor SgrR Coordinates the Response to Glucose-Phosphate Stress J. Bacteriol., March 15, 2007; 189(6): 2238 - 2248. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Mandin, F. Repoila, M. Vergassola, T. Geissmann, and P. Cossart Identification of new noncoding RNAs in Listeria monocytogenes and prediction of mRNA targets Nucleic Acids Res., February 16, 2007; 35(3): 962 - 974. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. H. Urban and J. Vogel Translational control and target recognition by Escherichia coli small RNAs in vivo Nucleic Acids Res., February 16, 2007; 35(3): 1018 - 1037. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Tjaden, S. S. Goodwin, J. A. Opdyke, M. Guillier, D. X. Fu, S. Gottesman, and G. Storz Target prediction for small, noncoding RNAs in bacteria. Nucleic Acids Res., January 1, 2006; 34(9): 2791 - 2802. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Barreto, E. Jedlicki, and D. S. Holmes Identification of a Gene Cluster for the Formation of Extracellular Polysaccharide Precursors in the Chemolithoautotroph Acidithiobacillus ferrooxidans Appl. Envir. Microbiol., June 1, 2005; 71(6): 2902 - 2909. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Ostberg, I. Bunikis, S. Bergstrom, and J. Johansson The Etiological Agent of Lyme Disease, Borrelia burgdorferi, Appears To Contain Only a Few Small RNA Molecules J. Bacteriol., December 15, 2004; 186(24): 8472 - 8477. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Opdyke, J.-G. Kang, and G. Storz GadY, a Small-RNA Regulator of Acid Response Genes in Escherichia coli J. Bacteriol., October 15, 2004; 186(20): 6698 - 6705. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. E. Trotochaud and K. M. Wassarman 6S RNA Function Enhances Long-Term Cell Survival J. Bacteriol., August 1, 2004; 186(15): 4978 - 4985. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Valverde, M. Lindell, E. G. H. Wagner, and D. Haas A Repeated GGA Motif Is Critical for the Activity and Stability of the Riboregulator RsmY of Pseudomonas fluorescens J. Biol. Chem., June 11, 2004; 279(24): 25066 - 25074. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Masse, F. E. Escorcia, and S. Gottesman Coupled degradation of a small regulatory RNA and its mRNA targets in Escherichia coli Genes & Dev., October 1, 2003; 17(19): 2374 - 2383. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Adhya Suboperonic Regulatory Signals Sci. Signal., June 3, 2003; 2003(185): pe22 - pe22. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Meinken, H.-M. Blencke, H. Ludwig, and J. Stulke Expression of the glycolytic gapA operon in Bacillus subtilis: differential syntheses of proteins encoded by the operon Microbiology, March 1, 2003; 149(3): 751 - 761. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Gottesman Stealth regulation: biological circuits with small RNA switches Genes & Dev., November 15, 2002; 16(22): 2829 - 2842. [Full Text] [PDF]
|
|
