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Vol. 16, No. 17, pp. 2300-2312, September 1, 2002

RESEARCH PAPER
RNA chaperone StpA loosens interactions of the tertiary structure in the td group I intron in vivo

Christina Waldsich, Rupert Grossberger, and Renée Schroeder1

Institute of Microbiology and Genetics, University of Vienna, Vienna Biocenter, A-1030 Vienna, Austria

Efficient splicing of the td group I intron in vivo is dependent on the ribosome. In the absence of translation, the pre-mRNA is trapped in nonnative-splicing-incompetent conformations. Alternatively, folding of the pre-mRNA can be promoted by the RNA chaperone StpA or by the group I intron-specific splicing factor Cyt-18. To understand the mechanism of action of RNA chaperones, we probed the impact of StpA on the structure of the td intron in vivo. Our data suggest that StpA loosens tertiary interactions. The most prominent structural change was the opening of the base triples, which are involved in the correct orientation of the two major intron core domains. In line with the destabilizing activity of StpA, splicing of mutant introns with a reduced structural stability is sensitive to StpA. In contrast, Cyt-18 strengthens tertiary contacts, thereby rescuing splicing of structurally compromised td mutants in vivo. Our data provide direct evidence for protein-induced conformational changes within catalytic RNA in vivo. Whereas StpA resolves tertiary contacts enabling the RNA to refold, Cyt-18 contributes to the overall compactness of the td intron in vivo.

[Key Words: Group I intron; RNA structure; in vivo splicing; DMS probing in vivo; RNA chaperone StpA; aminoacyl-tRNA-synthetase Cyt-18]

1 Corresponding author.

GENES & DEVELOPMENT 16:2300-2312 © 2002 by Cold Spring Harbor Laboratory Press  ISSN 0890-9369/02 $5.00
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