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RESEARCH PAPER

Loss of circadian rhythmicity in aging mPer1^-/- mCry2^-/- mutant mice

¹ Department of Medicine, Division of Biochemistry, University of Fribourg, 1700 Fribourg, Switzerland; ² Department of Cell Biology & Genetics, Erasmus Medical Centre Rotterdam, 3000 DR Rotterdam, The Netherlands

The mPer1, mPer2, mCry1, and mCry2 genes play a central role in the molecular mechanism driving the central pacemaker of the mammalian circadian clock, located in the suprachiasmatic nuclei (SCN) of the hypothalamus. In vitro studies suggest a close interaction of all mPER and mCRY proteins. We investigated mPER and mCRY interactions in vivo by generating different combinations of mPer/mCry double-mutant mice. We previously showed that mCry2 acts as a nonallelic suppressor of mPer2 in the core clock mechanism. Here, we focus on the circadian phenotypes of mPer1/mCry double-mutant animals and find a decay of the clock with age in mPer1^-/- mCry2^-/- mice at the behavioral and the molecular levels. Our findings indicate that complexes consisting of different combinations of mPER and mCRY proteins are not redundant in vivo and have different potentials in transcriptional regulation in the system of autoregulatory feedback loops driving the circadian clock.

[Keywords: Circadian clock; Per; Cry; aging; transcription]

Received November 28, 2002; revised version accepted April 10, 2003.

³ E-MAIL urs.albrecht{at}unifr.ch; FAX 41-26-300-9735.

Article and publication are at http://www.genesdev.org/cgi/doi/10.1101/gad.256103.

Supplemental material is available at http://www.genesdev.org.

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