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Vol. 17, No. 4, pp. 438-442, February 15, 2003
1 Center for Cancer Research, Department of Biology, and
2 McGovern Institute for Brain Research, Massachusetts
Institute of Technology, Cambridge, Massachusetts 02139, USA
With the discovery of RNA interference (RNAi)
and related phenomena, new regulatory roles attributed to RNA continue
to emerge. Here we show, in mammalian tissue culture, that a short
interfering RNA (siRNA) can repress expression of a target mRNA with
partially complementary binding sites in its 3' UTR, much like the
demonstrated function of endogenously encoded microRNAs (miRNAs). The
mechanism for this repression is cooperative, distinct from the
catalytic mechanism of mRNA cleavage by siRNAs. The use of siRNAs to
study translational repression holds promise for dissecting the
sequence and structural determinants and general mechanism of gene
repression by miRNAs.
[Keywords: siRNA; miRNA; RNAi; translational repression; 3' UTR]
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