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Vol. 17, No. 4, pp. 529-540, February 15, 2003
1 Department of Molecular, Cellular and Developmental
Biology, Yale University, New Haven, Connecticut 06520-8103, USA;
2 Department of Molecular Biophysics and Biochemistry, Yale
University, New Haven, Connecticut 06520-8114, USA;
3 Department of Medical Anesthesiology, Yale University, New
Haven, Connecticut 06520-8051, USA; 4 Department of Genetics,
Yale University, New Haven, Connecticut 06520-8005, USA
A DNA microarray representing nearly all of the unique sequences of
human Chromosome 22 was constructed and used to measure global-transcriptional activity in placental poly(A)+ RNA. We
found that many of the known, related and predicted genes are
expressed. More importantly, our study reveals twice as many transcribed bases as have been reported previously. Many of the newly
discovered expressed fragments were verified by RNA blot analysis and a
novel technique called differential hybridization mapping (DHM).
Interestingly, a significant fraction of these novel fragments are
expressed antisense to previously annotated introns. The coding
potential of these novel expressed regions is supported by their
sequence conservation in the mouse genome. This study has greatly
increased our understanding of the biological information encoded on a
human chromosome. To facilitate the dissemination of these results to
the scientific community, we have developed a comprehensive Web
resource to present the findings of this study and other features of
human Chromosome 22 at http://array.mbb.yale.edu/chr22.
[Keywords: Microarray; Chromosome 22; human genome; transcriptome; placental RNA; mouse homology]
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