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RESEARCH COMMUNICATION
-globin locus requires the transcription factor EKLF
Erasmus University Medical Center, Department of Cell Biology, The Netherlands
Three-dimensional organization of a gene locus is important for its regulation, as recently demonstrated for the 
-globin locus. When actively expressed, the cis-regulatory elements of the -globin locus are in proximity in the nuclear space, forming a compartment termed the Active Chromatin Hub (ACH). However, it is unknown which proteins are involved in ACH formation. Here, we show that EKLF, an erythroid transcription factor required for adult -globin gene transcription, is also required for ACH formation. We conclude that transcription factors can play an essential role in the three-dimensional organization of gene loci.
[Keywords: EKLF; chromatin; spatial organization; transcription factors; globin; ACH]
Received November 18, 2003; revised version accepted August 16, 2004.
Article and publication are at http://www.genesdev.org/cgi/doi/10.1101/gad.317004.
1 These authors contributed equally to this work.
2 Corresponding author. E-MAIL j.philipsen{at}erasmusmc.nl; FAX 31-10-4089468.
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