![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
RESEARCH PAPER
1 Center for Gene Regulation, Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania 16802, USA; 2 Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York 14853, USA
Pcf11 is one of numerous proteins involved in pre-mRNA 3'-end processing and transcription termination. Using elongation complexes (ECs) formed from purified yeast RNA polymerase II (Pol II), we show that a 140-amino acid polypeptide from yeast Pcf11 is capable of dismantling the EC in vitro. This action depends on the C-terminal domain (CTD) of the largest subunit of Pol II and the CTD-interaction domain (CID) of Pcf11. Our experiments reveal a novel termination mechanism whereby Pcf11 bridges the CTD to the nascent transcript and causes dissociation of both Pol II and the nascent transcript from the DNA in the absence of nucleotide hydrolysis. We posit that conformational changes in the CTD are transduced through Pcf11 to the nascent transcript to cause termination.
[Keywords: Polyadenylation; termination; RNA polymerase II; Pcf11; CTD]
Received January 6, 2005; revised version accepted May 23, 2005.
E-MAIL dsg11{at}psu.edu; FAX (814) 863-7024.
CiteULike 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  S. West and N. J. Proudfoot Human Pcf11 enhances degradation of RNA polymerase II-associated nascent RNA and transcriptional termination Nucleic Acids Res., February 11, 2008; 36(3): 905 - 914. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. S. Gilmour and R. Fan Derailing the Locomotive: Transcription Termination J. Biol. Chem., January 11, 2008; 283(2): 661 - 664. [Full Text] [PDF]
|
|
|
|
 |
|
 S. Kaneko, O. Rozenblatt-Rosen, M. Meyerson, and J. L. Manley The multifunctional protein p54nrb/PSF recruits the exonuclease XRN2 to facilitate pre-mRNA 3' processing and transcription termination Genes & Dev., July 15, 2007; 21(14): 1779 - 1789. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Zhang, A. Klatt, A. J. Henderson, and D. S. Gilmour Transcription termination factor Pcf11 limits the processivity of Pol II on an HIV provirus to repress gene expression Genes & Dev., July 1, 2007; 21(13): 1609 - 1614. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. L. Carroll, R. Ghirlando, J. M. Ames, and J. L. Corden Interaction of yeast RNA-binding proteins Nrd1 and Nab3 with RNA polymerase II terminator elements RNA, March 1, 2007; 13(3): 361 - 373. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Qu, J.-M. Perez-Canadillas, S. Agrawal, J. De Baecke, H. Cheng, G. Varani, and C. Moore The C-terminal Domains of Vertebrate CstF-64 and Its Yeast Orthologue Rna15 Form a New Structure Critical for mRNA 3'-End Processing J. Biol. Chem., January 19, 2007; 282(3): 2101 - 2115. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Nag, K. Narsinh, A. Kazerouninia, and H. G. Martinson The conserved AAUAAA hexamer of the poly(A) signal can act alone to trigger a stable decrease in RNA polymerase II transcription velocity RNA, August 1, 2006; 12(8): 1534 - 1544. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Gromak, S. West, and N. J. Proudfoot Pause Sites Promote Transcriptional Termination of Mammalian RNA Polymerase II Mol. Cell. Biol., May 15, 2006; 26(10): 3986 - 3996. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Rosonina, S. Kaneko, and J. L. Manley Terminating the transcript: breaking up is hard to do. Genes & Dev., May 1, 2006; 20(9): 1050 - 1056. [Full Text] [PDF]
|
|
|
|
|
|
W. Luo, A. W. Johnson, and D. L. Bentley The role of Rat1 in coupling mRNA 3'-end processing to transcription termination: implications for a unified allosteric-torpedo model Genes & Dev., April 15, 2006; 20(8): 954 - 965. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. WEST, K. ZARET, and N. J. PROUDFOOT Transcriptional termination sequences in the mouse serum albumin gene. RNA, April 1, 2006; 12(4): 655 - 665. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. HOLLINGWORTH, C. G. NOBLE, I. A. TAYLOR, and A. RAMOS RNA polymerase II CTD phosphopeptides compete with RNA for the interaction with Pcf11 RNA, April 1, 2006; 12(4): 555 - 560. [Abstract] [Full Text] [PDF]
|
|
