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Condensin is required for chromosome arm cohesion during mitosis

Department of Medical Genetics and Microbiology, University of Toronto, Toronto, Ontario M5S 1A8, Canada

We describe a novel requirement for the condensin complex in sister chromatid cohesion in Saccharomyces cerevisiae. Strikingly, condensin-dependent cohesion can be distinguished from cohesin-based pairing by a number of criteria. First, condensin is required to maintain cohesion at several chromosomal arm sites but, in contrast to cohesin, is not required at either centromere or telomere-proximal loci. Second, condensin-dependent interlinks are established during mitosis independently of DNA replication and are reversible within a single cell cycle. Third, the loss of condensin-dependent linkages occurs without affecting cohesin levels at the separated URA3 locus. We propose that, during mitosis, robust sister chromatid cohesion along chromosome arms requires both condensinand cohesin-dependent mechanisms, which function independently of each other. We discuss the implications of our results for current models of sister chromatid cohesion.

[Keywords: Condensin complex; cohesin complex; sister chromatid cohesion; chromosome segregation; mitotic chromosome structure]

Received July 13, 2006; revised version accepted August 31, 2006.

E-MAIL brigitte.lavoie{at}utoronto.ca; FAX (416) 978-6885.

Article is online at http://www.genesdev.org/cgi/doi/10.1101/gad.1468806.

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